Expression of progesterone receptor related to the polymorphism in the PGR gene in the rabbit reproductive tract1

Peiró, Rosa; Herrler, Andreas; Santacreu, M. A.; Merchán, M.; Argente, M. J.; García, María Luz; Folch, Josep María; Blasco, A.

doi:10.2527/jas.2009-1955

Cited by 17 publications

(23 citation statements)

References 33 publications

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“…Although both PRA and PRB bind P4, several studies have shown that, depending on the cell and target genes, PRA and PRB have remarkably different transcriptional activities [31]. However, little information is available so far with respect to the respective roles of PRA and PRB in mediating P4 action in the mammalian oviduct [32,33]. Nevertheless, in the present study, nuclear P4 receptors were expressed between Day 1.5 and Day 5 post-ovulation in both the ampulla and the isthmus.…”

Section: Discussionmentioning

confidence: 99%

Expression of nuclear progesterone receptor and progesterone receptor membrane components 1 and 2 in the oviduct of cyclic and pregnant cows during the post-ovulation period

Saint‐Dizier

Sandra

Ployart

et al. 2012

Reprod Biol Endocrinol

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BackgroundProgesterone (P4) may modulate oviductal functions to promote early embryo development in cattle. In addition to its nuclear receptor (PR), P4 may mediate its actions through P4 receptor membrane component 1 (PGRMC1) and its relative, PGRMC2. Two successive experiments were undertaken to characterise the expression of PR, PGRMC1 and PGRMC2 in the bovine oviduct during the post-ovulation period, and to relate their expression to the presence of an embryo, the proximity of the CL and to the region of the oviduct.MethodsIn the first experiment (Exp. I), whole oviduct sections were collected from Holstein cows at Day 1.5, Day 4 and Day 5 post-ovulation (n = 2 cows per stage). The expression of PR, PGRMC1 and PGRMC2 was studied in the ampulla and isthmus by RT-PCR, western-blot and immunohistochemistry. In Exp. II, oviduct epithelial cells were collected from cyclic and pregnant Charolais cows (n = 4 cows per status) at Day 3.5 post-ovulation and mRNA expression of PR, PGRMC1 and PGRMC2 was examined in the ampulla and isthmus by real-time quantitative PCR.ResultsIn Exp. I, PR, PGRMC1 and PGRMC2 were expressed in all oviduct samples. PGRMC1 was mainly localised in the luminal epithelium whereas PR and PGRMC2 were localised in the epithelium as well as in the muscle and stroma layers of the oviduct. The expression was primarily nuclear for PR, primarily cytoplasmic for PGRMC1 and both nuclear and cytoplasmic for PGRMC2. In Exp. II, mRNA levels for PR, PGRMC1 and PGRMC2 were not affected by either the pregnancy status or the side relative to the CL. However, the expression of PR and PGRMC2 varied significantly with the region of the oviduct: PR was more highly expressed in the isthmus whereas PGRMC2 was more highly expressed in the ampulla.ConclusionsThis is the first evidence of PGRMC2 expression in the bovine oviduct. Our findings suggest that P4 regulates the functions of the bovine oviduct in a region-specific manner and through both classical and non classical pathways during the post-ovulation period.

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Section: Discussionmentioning

confidence: 99%

Expression of nuclear progesterone receptor and progesterone receptor membrane components 1 and 2 in the oviduct of cyclic and pregnant cows during the post-ovulation period

Saint‐Dizier

Sandra

Ployart

et al. 2012

Reprod Biol Endocrinol

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“…Les études de gènes candidats pour les caractères de reproduction sont toutes fondées sur une seule population expérimentale sélectionnée de façon divergente pour la capacité uté-rine (nombre maximal de foetus qu'une lapine peut porter lorsque le taux d'ovulation n'est pas facteur limitant) pendant 10 générations (Mocé et al, 2004). Une association entre le polymorphisme de trois gènes (OVGP1, PGR et TIMP1) et quelques caractères de reproduction, incluant l'implantation embryonnaire et la taille de portée, a été mise en évidence dans cette population expérimentale (Estellé et al, 2006 ;Peiró et al, 2008 ;Peiró et al, 2010 ;Argente et al, 2010 ;García et al, 2010).…”

Section: Caractères De Reproductionunclassified

La génomique du lapin : avancées, applications et perspectives

Garreau

Gunia

2018

INRA Prod. Anim.

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L’évolution récente des technologies de séquençage et l’apport de la génomique a révolutionné nos connaissances sur les génomes et leurs polymorphismes, et permis d’élaborer des outils de génotypage qui accélèrent l’identification de polymorphismes causaux et contribuent à améliorer significativement le progrès génétique réalisé chez certaines espèces d’élevage, en particulier les bovins laitiers. Le séquençage complet du génome du lapin réalisé par le « Broad Institute » (Boston, USA), avec l’appui d’un consortium international auquel a contribué l’INRA, a été publié en 2014. Les résultats obtenus ont apporté un éclairage nouveau sur l’évolution et la domestication du lapin. En 2016, dans le cadre d’un projet Européen (COST Action TD1101 « A Collaborative European Network on Rabbit Genome Biology – RGB-Net »), une puce de génotypage avec 200000 SNP (« Single-Nucleotide Polymorphism ») a été développée, permettant de renouveler les approches de génétique chez le lapin. L’objet de cette synthèse est de faire le point sur les connaissances relatives au génome du lapin et d’établir un inventaire des gènes ou régions génomiques liés à certaines fonctions ou caractères d’intérêt dans cette espèce. Nous décrivons ici le principe des outils (cartes génétiques, puces SNP, séquençage) et des méthodes (détection de QTL, approche gènes candidats, identification de mutations causales) qui ont déjà été appliqués chez le lapin. Nous illustrons les perspectives d’utilisation des outils maintenant disponibles pour la sélection avec deux projets de recherche portant sur la résistance aux maladies et l’efficacité alimentaire. Une réflexion prospective sur la sélection génomique est également proposée.

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“…As the main differences between lines appeared in the first two generations, the implication of at least one major gene affecting the UC and embryo survival was suggested (Argente et al 2003). Several studies of candidate genes for litter size and its components, such as embryo development or survival, have been performed in the both divergently selected lines (Estelle et al 2006;Merchán et al 2009;Argente et al 2010;García et al 2010;Peiró et al 2010). The main results of these studies 4 indicated that polymorphisms in tissue inhibitor of metalloproteinase-1 (TIMP-1), oviductal glycoprotein-1 (OVGP1) and progesterone receptor (PGR) are associated with the differences in embryo survival between H and L lines.…”

Section: Introductionmentioning

confidence: 99%

“…2B), although the L line presented more differentially expressed genes (UBC, SEP15, TCP1, CLU, HSD17B4 and PGR) than the H line (RPS20, RPS4X, ENV and OVGP1). Interestingly, the OVGP1 and the PGR genes has been previously selected as candidate genes to explain the differences in early embryo survival and development observed between the divergent rabbit lines selected for UC using an F2 population (García et al 2010;Peiró et al 2010).…”

Section: Ssh Librariesmentioning

confidence: 99%

Identification of differentially expressed genes in the oviduct of two rabbit lines divergently selected for uterine capacity using suppression subtractive hybridization

et al. 2012

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This is the accepted version of the following article: Ballester, M.; Castelló, A.; Peiró Barber, RM.; Argente, M. J.;Santacreu Jerez, MA.; Folch, J. M. (2013). Identification of differentially expressed genes in the oviduct of two rabbit lines divergently selected for uterine capacity using suppression subtractive hybridization. Animal Genetics. SummarySuppressive subtractive hybridization (SSH) libraries from oviduct at 62h post-mating of two lines of rabbits divergently selected for uterine capacity were generated to identify differentially expressed genes. A total of 438 singletons and 126 contigs were obtained by cluster assembly and sequence alignment of 704 ESTs, from which 54% showed homology to known proteins of the non-redundant NCBI databases. Differential screening by dot-blot validated 71 ESTs, of which 47 showed similarity to known genes. Transcripts of genes were functionally annotated in the molecular function and the biological process gene ontology (GO) categories using the Blast2GO software and were assigned to reproductive developmental process, immune response, amino acid metabolism and degradation, response to stress and apoptosis terms. Finally, three interesting genes, PGR, HSD17B4 and ERO1Lwere identified as overexpressed in the Low line using RT-qPCR. Our study provides a list of candidate genes that can be useful to understand the molecular mechanisms underlying the phenotypic differences observed on early embryo survival and development traits.

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Expression of progesterone receptor related to the polymorphism in the PGR gene in the rabbit reproductive tract1

Cited by 17 publications

References 33 publications

Expression of nuclear progesterone receptor and progesterone receptor membrane components 1 and 2 in the oviduct of cyclic and pregnant cows during the post-ovulation period

Expression of nuclear progesterone receptor and progesterone receptor membrane components 1 and 2 in the oviduct of cyclic and pregnant cows during the post-ovulation period

La génomique du lapin : avancées, applications et perspectives

Identification of differentially expressed genes in the oviduct of two rabbit lines divergently selected for uterine capacity using suppression subtractive hybridization

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