Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle

Kurki, Pekka; Vanderlaan, Martin; Dolbeare, Frank; Gray, Joe W.; Tan, Eng M.

doi:10.1016/0014-4827(86)90520-3

Cited by 501 publications

(281 citation statements)

References 11 publications

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“…From these observations we conclude that PCNA distribution in the columns and shell during this early period reflects not only the actively dividing cell pool, but also indicates a rapidly migrating and differentiating cytotrophoblast population. For example, a cell that has just completed mitosis and cytokinesis in the proximal column is probably migrating distally (toward the shell) as it enters the GI-phase of the next cell cycle, when PCNA levels would be expected to increase (Kurki et al, 1986(Kurki et al, , 1988. Even if the trophoblast cell exits the cell cycle and differentiates, entering Go, the half-life of PCNA is sufficiently long (approximately 20 hr; Bravo and Macdonald-Bravo, 1987) that these cells would continue to be labeled for some time.…”

Section: Discussionmentioning

confidence: 99%

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Blankenship

King

1994

Developmental Dynamics

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Placental growth is largely determined by the proliferation of cytotrophoblast cells. However, the distribution of cytotrophoblast cells engaged in the cell cycle during placental development is poorly understood. Recently, antibodies have been developed that identify two proteins directly involved with DNA synthesis: Ki-67 protein and proliferating cell nuclear antigen (PCNA). Immunolocalization of Ki-67 and PCNA provides a measure of the proliferating cells in tissues. We examined, in macaque placentas, the spatiotemporal pattern of expression of these proteins during gestation. Tissues from 24 macaque placentas collected from 22-153 days of pregnancy were prepared for paraffin sections. Standard immunoperoxidase techniques were used to identify Ki-67 and PCNA. The proteins generally co-localized, although PCNA was usually represented in more cells than Ki-67. Early in gestation the cell columns contained many labeled cells. The cytotrophoblastic shell was occupied by numerous cells with PCNA positive nuclei, but few were reactive for Ki-67. By 45 days of pregnancy the immunolabeled cells in the cell columns were concentrated in the proximal regions, adjacent to the anchoring villus tips. The number of positive cells decreased by 100 days when the cell columns were diminished, leaving the anchoring villus tips buried in the shell. Labeled cells were rarely present in the shell at late pregnancy. The single layer of cytotrophoblast cells in the chorionic plate contained numerous reactive cells throughout early and mid-gestation. After approximately 100 days the cytotrophoblast layer of the chorionic plate was stratified over large areas. Soon thereafter few cells of the chorionic plate were labeled. The chorionic villi contained reactive cytotrophoblastic cells throughout gestation. Extravillous cytotrophoblast cells invading spiral arteries were sometimes labeled for PCNA but not Ki-67. We conclude that compartments of the placenta are distinguished by specific patterns of cytotrophoblast cell proliferation. Moreover, these patterns correspond to macroscopic growth parameters of the placenta. Evidence suggests that the macaque placenta slows its rate of diametrical growth at approximately 100 days of gestation. It is at about this time that the cell columns are absorbed into the trophoblastic shell and this pool ofproliferating cells is diminished. The growth in diameter of the chorionic plate matches that of the shell. In this compartment also the architecture changes at about 100 days as the cytotrophoblast layer stratifies. This stratification may result from continued proliferation of cytotrophoblast cells when the diametrical rate of growth is decreasing. Soon thereafter, proliferation decreases in this compartment also. By contrast, labeled cells were found in chorionic villi throughout gestation. Continued villous growth and branching probably accounts for most of the increases in placental thickness and weight that occurs during late gestation. 0 1994 Wiley-Liss, Inc.

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Section: Discussionmentioning

confidence: 99%

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Blankenship

King

1994

Developmental Dynamics

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“…A 530-nm band pass-filter was used for FITC fluorescence and a dichroic mirror, DM570, and a 600-nm band pass-filter were used for red (propidium iodide) fluorescence. Details of flow cytometry in the detection of nuclear autoantigens are described elsewhere (19). -staining (e, f, g, and h, respectively).…”

Section: Methodsmentioning

confidence: 99%

Monoclonal autoantibody from a (new zealand black x new zealand white)F₁ mouse and some human scleroderma sera target an MT_r 34,000 nucleolar protein of the U3 RNP particle

Reimer

Pollard

Penning

et al. 1987

Arthritis & Rheumatism

256

139

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Monoclonal antibodies against nuclear antigens, such as DNA, histones, Sm, U1 RNP, and SS-B (La), which are targets of spontaneously occurring autoantibodies in systemic rheumatic diseases, have recently been produced by several investigators (1-5). The significance of using monoclonal antibodies against nuclear autoantigens lies in their monospecificity , as compared with human autoimmune sera, which frequently target more than 1 nuclear antigen (6). Therefore, monoclonal antinuclear antibodies should be important probes not only for standardization of clinical tests, but also for further dissecting the structure and function of reactive antigens. In a recent study, we showed that monoclonal anti-native DNA (anti-nDNA) antibodies also reacted with mitochondrial DNA in tissue culture cells (7). This observation facilitated studies to determine why certain human systemic lupus erythematosus sera that display high titers of anti-nDNA antibodies stain mitochondria in tissue culture cells (7).In the present study, we have shown that a monoclonal autoantibody derived from an autoimmune (New Zealand black X New Zealand white)F1 ([NZB/NZW]F1) mouse reacts with an M , 34,000 nucleolar protein. This protein was demonstrated to share an epitope which is also recognized by scleroderma antibodies reactive with an M , 34,000 nucleolar protein associated with the U3 RNP (8,9). MATERIALS AND METHODSMonoclonal antibodies. Monoclonal antibodies were produced by hybridoma technology as described previously (10). Splenocytes from a 6-month-old female, unmanipulated (NZBMZW)FI mouse were mixed, at a ratio of lO:l, with nonsecreting myeloma cells (P3x63Ag8.653) and fused with 35% polyethylene glycol. Hybrids were selected in hypoxanthine/aminopteridthymidine-containing medium, as described (10). After propagating hybridomas and subcloning

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“…Different anti-PCNA antibodies were used: a well defined autoantiserum obtained from a patient (AK) with systemic lupus erythematosus (SLE) (21,24,40,42) and three monoclonal antibodies (moabs), 19F4 (IgG1) (311, TOB7 (IgG,) (411, and PClO (IgG,,) (43). The AK serum, 19F4, and TOB7 antibodies were kindly supplied by Dr. E.M. Tan, La Jolla, CA.…”

Section: Antibodiesmentioning

confidence: 99%

Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells

Landberg

Roos

1992

Cytometry

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The expression of different proliferation associated nuclear antigens was analyzed using a washless double-staining method and flow cytometry. It is a simple and rapid two-step procedure which can be performed on low cell numbers. A series of hematopoietic cell lines and fresh lymphoma cells were tested and the methodology was found to be applicable to a number of nuclear antigens (PCNA, Ki-67, p105, MPM-2, fibrillarin). For PCNA, the detectability was dependent on the type of antibody used. The immunofluorescence pattern observed by microscopy was altered for antigens stained by the washless technique in comparison with the pattern obtained with fixed cells. With the washless method, detailed cell cycle analysis could be obtained by dual parameter analysis of PCNA and Ki-67.

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Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle

Cited by 501 publications

References 11 publications

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Monoclonal autoantibody from a (new zealand black x new zealand white)F₁ mouse and some human scleroderma sera target an MT_r 34,000 nucleolar protein of the U3 RNP particle

Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells

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Expression of proliferating cell nuclear antigen (PCNA)/cyclin during the cell cycle

Cited by 501 publications

References 11 publications

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Developmental expression of Ki‐67 antigen and proliferating cell nuclear antigen in macaque placentas

Monoclonal autoantibody from a (new zealand black x new zealand white)F1 mouse and some human scleroderma sera target an MTr 34,000 nucleolar protein of the U3 RNP particle

Flow cytometric analysis of proliferation associated nuclear antigens using washless staining of unfixed cells

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Monoclonal autoantibody from a (new zealand black x new zealand white)F₁ mouse and some human scleroderma sera target an MT_r 34,000 nucleolar protein of the U3 RNP particle