Expression of PTPIP51 during mouse eye development

Abstract

The expression pattern of the novel tyrosine phosphatase interacting protein 51 (PTPIP51) was studied during the organogenesis of mouse eye on a transcriptional (RT-PCR and in situ hybridization) and translational level (immunohistochemistry and immunoblotting). Timed developmental stages from day E12 to day E18 were analyzed regarding the distribution of PTPIP51 and compared to the expression patterns observed during postnatal developmental stages and adult eye. PTPIP51 was found to be expressed in all investigated developmental stages in derivatives of mesoderm and ectoderm, such as developing cornea, lens, neuroretina and extraocular muscles. Conjuctiva and corneal epithelia were PTPIP51 reactive during all investigated developmental stages including the mature eye. Embryonic differentiation led to reactive keratocytes of the corneal stroma and remained so in post partal stages, as well as in the adult eye. On day E12, all cells comprising the developing lens body showed PTPIP51 expression. Further development unto the adult eye resulted in a restriction of PTPIP51 expression to the anterior lens epithelium and finally to the equatorial region of the lens epithelium. The developing neuroretina showed a strong PTPIP51 expression in the inner neuroblastic layer and the future receptor cell layer. In the adult eye, the retinal ganglion cells and the inner nuclear layer remained PTPIP51 reactive. The data presented here suggests PTPIP51 to be integrated in signaling cascades regulating differentiation and apoptosis during eye development.

Protein Tyrosine Phosphatase Interacting Protein 51 (PTPIP51) mRNA Expression and Localization and Its In Vitro Interacting Partner Protein Tyrosine Phosphatase 1B (PTP1B) in Human Placenta of the First, Second, and Third Trimester

“…A regulated layer-dependent expression pattern was particularly observed for epidermal and seminiferous epithelia, both requiring a carefully controlled balance between proliferation, dif-ferentiation, and apoptosis (Nickoloff and Denning 2001;Stenzinger et al 2005Stenzinger et al ,2006. Moreover, a study conducted by Märker et al (2008) pointed to a crucial role for PTPIP51 during mouse eye development. In accordance with these data, Roger et al (2007) described a stage-dependent, biphasic expression pattern of Ptpip51 in postnatal retinal development, which is governed by ciliary neurotrophic factor (CNTF), a member of the interleukin-6 family that mediates its action by the Janus-kinase/signal transducers and activators of transcription (JAK-STAT) signaling pathways.…”

Protein tyrosine phosphatase interacting protein 51—a jack-of-all-trades protein

“…Interestingly, PTPIP51 protein contains a transmembrane domain (TMD) that is essential for its translocation to mitochondria and the induction of apoptosis. This association of PTPIP51 with apoptotic processes has also been observed in vivo; however, a restriction solely to apoptotic functions has been excluded Maerker et al 2008).…”

“…The screening of embryonic tissue samples at various stages of murine eye development has stressed the importance of PTPIP51 expression during the maturation of this organ (Maerker et al 2008). Apoptotic cells labelled by the TdT-mediated dUTP-biotin nick-end labelling assay display no exclusive association with the PTPIP51 protein.…”

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“…To elucidate a possible role of PTPIP51 in eye development, Maerker et al (2008) studied the expression of PTPIP51 during the organogenesis of the mouse eye using RT-PCR and in situ hybridization, immunohistochemistry and Western blotting. They succeeded in demonstrating a distinct spatial and temporal expression of the protein on a transcriptional and translational level in the developing cornea, lens, neuroretina and extraocular muscles of the mouse eye during embryogenesis, in postnatal stages and in the mature tissues in the adult animal.…”