Expression of RBMX in the Light-Induced Damage of Rat Retina In Vivo

Dai, Ming; Liu, Yonghua; Nie, Xiaobing; Zhang, Jinlong; Wang, Yong; Ben, Jindong; Zhang, Su; Yang, Xiaowei; Sang, Aimin

doi:10.1007/s10571-014-0140-z

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…Previous studies have implicated light-induced damage in the progression of retinal degeneration focusing on the consequences of light-induced damage on cell function and homeostasis in the retina, noting changes in neural morphology, photoreceptor responsiveness, along with the induction of apoptosis 14 15 16 . It is well known that a consequence of photo-oxidative stress is an increase in the production of reactive lipid aldehydes, primarily 4HNE, leading to lipid peroxidation of retinal tissues and cytotoxic protein modifications in retinal cells 17 .…”

Section: Resultsmentioning

confidence: 99%

A novel AhR ligand, 2AI, protects the retina from environmental stress

Gutierrez

Davis

Rosko

et al. 2016

Sci Rep

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Various retinal degenerative diseases including dry and neovascular age-related macular degeneration (AMD), retinitis pigmentosa, and diabetic retinopathy are associated with the degeneration of the retinal pigmented epithelial (RPE) layer of the retina. This consequently results in the death of rod and cone photoreceptors that they support, structurally and functionally leading to legal or complete blindness. Therefore, developing therapeutic strategies to preserve cellular homeostasis in the RPE would be a favorable asset in the clinic. The aryl hydrocarbon receptor (AhR) is a conserved, environmental ligand-dependent, per ARNT-sim (PAS) domain containing bHLH transcription factor that mediates adaptive response to stress via its downstream transcriptional targets. Using in silico, in vitro and in vivo assays, we identified 2,2′-aminophenyl indole (2AI) as a potent synthetic ligand of AhR that protects RPE cells in vitro from lipid peroxidation cytotoxicity mediated by 4-hydroxynonenal (4HNE) as well as the retina in vivo from light-damage. Additionally, metabolic characterization of this molecule by LC-MS suggests that 2AI alters the lipid metabolism of RPE cells, enhancing the intracellular levels of palmitoleic acid. Finally, we show that, as a downstream effector of 2AI-mediated AhR activation, palmitoleic acid protects RPE cells from 4HNE-mediated stress, and light mediated retinal degeneration in mice.

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Section: Resultsmentioning

confidence: 99%

A novel AhR ligand, 2AI, protects the retina from environmental stress

Gutierrez

Davis

Rosko

et al. 2016

Sci Rep

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“…A previous study found that loss of mitochondrial DARS2 leads to the activation of various stress responses in a tissue-specific manner independently of respiratory chain deficiency [ 27 ]. The RBMX is a nuclear protein that is involved in alternative splicing of RNA [ 28 ]. It can confer resistance to DNA damage [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Proteome and microbiota analysis reveals alterations of liver-gut axis under different stocking density of Peking ducks

Qin

et al. 2018

PLoS ONE

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This study aimed to determine the impact of stocking density on the liver proteome and cecal microbiota of Peking ducks. A total of 1,200 21-day-old ducks were randomly assigned to 5 stocking density groups of 5, 6, 7, 8 and 9 ducks/m2, with 6 replicates for each group. At 40 days of age, duck serum and pectorals were collected for biochemical tests; liver and cecal contents of ducks were gathered for proteome and microbiota analysis, respectively. Serum MDA increased while pectorals T-AOC reduced linearly with enhancing stocking density. Duck lipid metabolism was altered under different stocking density as well. Serum LDL-C increased linearly with increasing stocking density. Proteome analysis revealed fatty acid biosynthesis proteins such as acyl-CoA synthetase family member 2 and fatty acid oxidation related proteins including acyl-CoA dehydrogenase long chain and acyl-coenzyme A oxidase were enriched in high stocking density group. Additionally, high stocking density increased oxidative response associated proteins such as DDRGK domain containing 1. Furthermore, increasing stocking density diminished proteins of anti-oxidant capacity including regucalcin and catalase. 16S rDNA analysis revealed that higher stocking density was accompanied with decreased microbial diversity, as well as depletion of anti-inflammatory bacterial taxa, including Bacteroidales, Butyricimonas and Alistipe. Besides, reduced bile acid metabolism-associated bacteria such as Ruminococcaceae, Clostridiales and Desulfovibrionaceae were found in the high-density group. Both proteome and 16S rDNA results showed inflammation and chronic liver disease trend in the high-density group, which suggests the involvement of the liver-gut axis in oxidative stress.

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“…139 Cleaved caspase-3 is elevated in RGC 6 h after toxic light exposure and reaches a peak after 3 days, 140–142 co-localising with increased staining for Ras homologue enriched in the brain (RHEB), cyclic AMP response element modulator-1 (CREM-1), transcription initiator factor IIB (TFIIB), pyruvate kinase isozyme type M2 (PKM2), SYF2 pre-mRNA splicing factor (SYF2) and RNA-binding motif protein, X-linked (RBMX), which are all involved in cell death pathways. 140–145 Nuclear factor of activated T cells, cytoplasmic 4 (NFATc4) (a component of T-cell activation and a regulator of the immune response) are also co-localised with cleaved caspase-3, caspase-8 and Fas-L in RGC, suggesting that NFATc4 may upregulate Fas-L and participate in RGC apoptosis. 146 Intravitreal mitogen-activated protein kinases/extracellular signal-regulated kinases (MAPK/ERK) inhibitor reduces PKM2 and active caspase-3 protein expression, suggesting that light-induced RGC apoptosis is in part dependent on MAPK/ERK pathway.…”

Section: Caspases and Rgc Deathmentioning

confidence: 99%

Caspases in retinal ganglion cell death and axon regeneration

et al. 2017

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Retinal ganglion cells (RGC) are terminally differentiated CNS neurons that possess limited endogenous regenerative capacity after injury and thus RGC death causes permanent visual loss. RGC die by caspase-dependent mechanisms, including apoptosis, during development, after ocular injury and in progressive degenerative diseases of the eye and optic nerve, such as glaucoma, anterior ischemic optic neuropathy, diabetic retinopathy and multiple sclerosis. Inhibition of caspases through genetic or pharmacological approaches can arrest the apoptotic cascade and protect a proportion of RGC. Novel findings have also highlighted a pyroptotic role of inflammatory caspases in RGC death. In this review, we discuss the molecular signalling mechanisms of apoptotic and inflammatory caspase responses in RGC specifically, their involvement in RGC degeneration and explore their potential as therapeutic targets.

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Expression of RBMX in the Light-Induced Damage of Rat Retina In Vivo

Cited by 10 publications

References 30 publications

A novel AhR ligand, 2AI, protects the retina from environmental stress

A novel AhR ligand, 2AI, protects the retina from environmental stress

Proteome and microbiota analysis reveals alterations of liver-gut axis under different stocking density of Peking ducks

Caspases in retinal ganglion cell death and axon regeneration

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