1993
DOI: 10.1006/bmmb.1993.1055
|View full text |Cite
|
Sign up to set email alerts
|

Expression of Recombinant Human Methylmalonyl-CoA Mutase: In Primary mut Fibroblasts and Saccharomyces cerevisiae

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1
1

Citation Types

0
19
0

Year Published

1997
1997
2006
2006

Publication Types

Select...
6
1

Relationship

0
7

Authors

Journals

citations
Cited by 34 publications
(19 citation statements)
references
References 0 publications
0
19
0
Order By: Relevance
“…Feeding RNAi was performed as described [23]. The strains were grown overnight from individual colonies in LB with 100 µg/ ml of ampicillin at 37° C. One milliliter of overnight culture was then placed in 30 ml of LB with 100 µg/ml of ampicillin and grown to OD of 0.4 absorbance units at 595 nm.…”
Section: Rna Interferencementioning
confidence: 99%
See 2 more Smart Citations
“…Feeding RNAi was performed as described [23]. The strains were grown overnight from individual colonies in LB with 100 µg/ ml of ampicillin at 37° C. One milliliter of overnight culture was then placed in 30 ml of LB with 100 µg/ml of ampicillin and grown to OD of 0.4 absorbance units at 595 nm.…”
Section: Rna Interferencementioning
confidence: 99%
“…A total of 1 to 3 g of freshly washed adult worms were washed twice with 10 ml MSM-E [150 ml MSM (40.08 g mannitol, 23.96 g sucrose, 1.047 g MOPS, 3 ml 0.1M EDTA, pH 7.4 per liter] and resuspended by centrifugation (300 × g, 10 min, 4°C). A glass Potter/Elvehjem tissue grinder with a Teflon (DuPont, Wilmington, DE) pestle (Wheaton, Millville, NJ) was then used for initial rupture: 3 slow strokes at 400 rpm.…”
Section: Mitochondrial Preparationsmentioning
confidence: 99%
See 1 more Smart Citation
“…Crane and Ledley examined selected mutant cDNAs in a yeast over-expression system [36] while others have used bacterial hosts to produce the enzyme [40,41]. The advantage of the yeast system is that the enzyme can be expressed with very high specific activities under standard growth conditions and can be processed and targeted to the mitochondria as it would in a vertebrate [42]. Kinetic analysis can be performed with either approach, although purified enzyme is always the preferred starting material for Km and Vmax determination.…”
Section: Methylmalonyl-coa Mutase (Mcm)mentioning
confidence: 99%
“…Yeast can produce a functional MCM apoenzyme when the gene is expressed from an inducible promoter [42]. Other than MCM, genes in the pathway have not been assayed for function using yeast overexpression systems to date.…”
Section: Yeastmentioning
confidence: 99%