1990
DOI: 10.1007/bf00404804
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Expression of reg protein in rat regenerating islets and its co-localization with insulin in the Beta cell secretory granules

Abstract: Regenerating islets can be induced by the administration of poly(ADP-ribose) synthetase inhibitors to 90% depancreatized rats. In screening a regenerating islet-derived cDNA library, we previously isolated a novel gene. reg (regenerating gene), which encodes a 165-amino acid protein with a 21-amino acid signal sequence. In the present study, we have examined the expression and localization of reg protein in the regenerating islets by immunocytochemical techniques using a monoclonal antibody against a recombina… Show more

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Cited by 126 publications
(96 citation statements)
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“…Under physiological conditions, Reg protein is not expressed in pancreatic ␤ cells (3,4,17), although the Reg protein receptor is expressed (8). In the regenerative process of pancreatic islets, Reg gene expression is induced (3,4,12,17).…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…Under physiological conditions, Reg protein is not expressed in pancreatic ␤ cells (3,4,17), although the Reg protein receptor is expressed (8). In the regenerative process of pancreatic islets, Reg gene expression is induced (3,4,12,17).…”
Section: Discussionmentioning
confidence: 99%
“…USA, 10.1073͞pnas.240458597. Article and publication date are at www.pnas.org͞cgi͞doi͞10.1073͞pnas.240458597 antibody (17) or anti-PARP antibodies (C-2-10, CLONTECH; 06-557, Upstate Biotechnology, Lake Placid, NY).…”
Section: Methodsmentioning
confidence: 99%
“…The monoclonal mouse IgG2a antibody raised against rat Reg I, which was confirmed to cross-react to the mouse Reg I protein (Terazono et al, 1990), was employed for immunohistochemistry. Monoclonal IgG biotinylated antimouse Musashi-1 antibody (Kaneko et al, 2000) was kindly provided by Dr H Okano (Department of Physiology, Keio University School of Medicine, Tokyo, Japan).…”
Section: Antibodiesmentioning
confidence: 99%
“…Clones B7-1, B8-1 and B10-2 showed a high degree of homology to the 3'-end of Reg gene which has been found in regenerating islets [14,15] and regenerating wrapped pancreas [6]. Reg protein has been shown, under certain circumstances, to be able to initiate cell growth in the rat [16].…”
Section: Discussionmentioning
confidence: 99%