Expression of selected nuclear genes during leaf development in barley

Abstract

Groups of cDNA clones encoding abundant leaf proteins or derived from genes (gene families) with other features of interest have been selected from a barley leaf cDNA library. The characteriaztion of nine of the groups is summarized and includes information on the tissue specificity and light dependence of expression of their corresponding genes. Different types of control of gene expression are represented in the collection: leaf-specific expression, both stimulated and inhibited by light, constitutive expression, and expression that is maximal in one case in coleoptiles and in two cases in meristematic tissue. For the light-stimulated genes (gene families) encoding chloroplast proteins (Cab, RbcS, and plastocyanin), relative and absolute levels of messengers were determined a5 a Cuuricliuii UC cell age in sections of 7-day-old barley leaves grown under diurnal conditions. Key parameters of cell growth (protein, RNA, and DNA accumulation) were determined in the same leaf sections. The main conclusions of the expression studies are as follows: 1) Light is in no case a requirement for gene expression although it has significant stimulatory effect on some genes: 2) weak expression of some genes coding for chloroplast proteins was detected in the leaf-like, white coleoptiles, whereas expression in roots could not be detected: 3) The cub, rbcS, and plastocyanin genes are expressed very early during leaf cell differentiation, when the plastids morphologically are still in their amyloplast-amoeboid stages: 4) The expression of the cub, rbcS, and plastocyanin genes is not coordinated during leaf cell development.

Differential Effects of Methyl Jasmonate on the Expression of the Early Light-Inducible Proteins and Other Light-Regulated Genes in Barley

“…In the non-JA-Me-treated controls, a small amount of leaf thionin was present (Fig. 6A) in contrast to other JIPs, which were not detectable (Gausing, 1987;Reimann-Philipp et al, 1989).…”

“…Signals were corrected for background noise. The following 32 P-labeled cDNA probes were used: ELIP of low (E L ϭ pHV90; Grimm et al, 1989) and high (E H ϭ pHV8F6; Kruse and Kloppstech, 1992) molecular mass, SSU (pKG4626; Barkadottir et al, 1987), LHC II (pKG1490; Barkadottir et al, 1987), andthionin (pKG1348;Gausing, 1987).…”

Effects of light stress on the expression of early light‐inducible proteins in barley

“…The plant material was incubated in the extraction buffer originally described [25], however, containing 2% SDS and 200 pglml proteinase K. After 30 min of incubation, the suspension was clarified by centrifugation, filtered when necessary and NaCl was added to 0.4 M. Oligo(dT) binding and washing were as described. Dot blotting of RNA on replica filters and hybridization using 32P-labeled random-primed homologous cDNA inserts of ELIP (p9022 [4]), LHC I1 (pKG1490 [27]) and SSU (pKG4626 [27]) were performed as described [28]. Quantification of signals from hybridized filters was performed by measuring Cerenkov radiation for excised dots.…”

The regulation of NADPH-protochlorophyllide oxidoreductases A and B in green barley plants kept under a diurnal light/dark cycle

“…This increase in PorA mRNA concentration is not confined to the lower part of the barley leaf with its basal meristem that gives rise to etiolated leaf cells when kept in the dark but also occurs in the upper part of the leaf. In this section of the leaf, only mature cells with fully developed chloroplasts are present (Boffey et al 1980;Dehesh et al 1983;Barkadottir et al 1987). During a dark period of 12-24 h, no changes in the thylakoid membrane system and reformation of prolamellar bodies were detectable.…”