Expression of seven members of the ADAM family in developing chicken spinal cord

Abstract

The expression patterns of seven members of the ADAM (a disintegrin and metalloprotease) family, including ADAM9, ADAM10, ADAM12, ADAM13, ADAM17, ADAM22, and ADAM23, were analyzed in the developing chicken lumbar spinal cord by in situ hybridization and immunohistochemistry. Results show that each individual ADAM is expressed and regulated spatiotemporally in the lumbar cord and its surrounding tissues. ADAM9, ADAM10, ADAM22, and ADAM23 are expressed predominantly by motoneurons in the motor column and by sensory neurons in the dorsal root ganglia, each with a different expression pattern. ADAM12 and ADAM13 are mainly expressed in the meninges around the lumbar cord and in the condensed sheets of chondroblasts around the vertebrae. ADAM17 expression is strong in the ventricular layer and limited to early stages. The differential expression of the ADAMs in the lumbar cord suggests that the ADAMs play a regulatory role in development of the spinal cord. Developmental Dynamics 239:1246-1254,

Quantitative and dynamic expression profile of premature and active forms of the regional ADAM proteins during chicken brain development

“…ADAM12-S is weakly expressed at the mRNA level measured by RT-PCR [17]. In the present study, our data show that the premature form of 121 kDa and the mature form of 75 kDa of the ADAM12 protein are detectable in all parts of the developing brain, whereas the premature form of 90 kDa of ADAM12-S is only detected in the diencephalon from E12 onward (Fig.…”

“…Two splice isoforms of chicken ADAM12, the normal ADAM12 and ADAM12-S, have been found [16,17]. ADAM12-S is weakly expressed at the mRNA level measured by RT-PCR [17].…”

“…1C, lane 4). Recently, a short splice isoform of chicken ADAM12 (ADAM12-S) was found and is weakly expressed in the developing brain measured by reverse transcription-polymerase chain reaction (RT-PCR) [17]. The predicted full-length amino acid sequence of this ADAM12-S is about 76 kDa.…”

“…During central nervous system (CNS) development, members of the ADAMs are widely expressed in distinct anatomical structures and play a pivotal role in neuronal proliferation and differentiation, cell migration, axon outgrowth and guidance, and synaptogenesis [4,9,10]. For example, several members of the ADAMs, including ADAM9, ADAM10, ADAM12, ADAM17, ADAM22 and ADAM23, are spatially and temporally regulated in distinct anatomical structures of the developing chicken brain and/or spinal cord [16,17]. ADAM10 sheds Notch ligands and regulates Notch signaling, which plays a critical role in embryonic development [18].…”

Expression of delta‐protocadherins in the spinal cord of the chicken embryo

“…The dorsal root ganglia and sympathetic ganglia, which are found in the vicinity of the spinal cord, are derived from the trunk neural crest (Le Douarin and Kalcheim, 1999;Kasemeier-Kulesa et al, 2005). Many genes, such as bone morphogenetic proteins, semaphorins, Eph/ephrins, and the ADAM family of molecules are involved in the formation of the spinal cord and its associated ganglia (Krull, 2001;Graham, 2003;Lin et al, 2010a).…”