Expression of sirtuins in ovarian follicles of postnatal mice

Abstract

Mammalian ovarian follicular development is an intricate, elaborate, and wellorganized phenomenon regulated by various signaling pathways; however, the underlying mechanism remains unclear. Mammalian sirtuins (sirtuin 1 to sirtuin 7) are a group of NAD +-dependent deacetylases implicated in various physiological processes including cell proliferation, apoptosis, cell cycle progression, and insulin signaling. Mammalian ovarian sirtuins have been studied using adult and aged bovine, porcine, and murine models. However, limited information is available regarding their precise expression patterns and the localization of follicle development in mice. This study aimed to assess the dynamic expression and localization of all seven sirtuins in early postnatal mouse ovaries through real-time polymerase chain reaction analysis and immunohistochemistry, respectively. During postnatal ovarian follicle development, sirtuin 1, sirtuin 4, and sirtuin 6 were downregulated compared with those in 1-day postnatal mouse ovaries (p < .05), indicating that these three sirtuin genes may be markers of follicular development. Combining their localization in granulosa cells through immunohistochemical studies, sirtuin 1, sirtuin 4, and sirtuin 6 are suggested to play negative regulatory roles in mammal ovarian follicular granulosa cell development. Furthermore, we found that sirtuin 2 (p < .05) and sirtuin 7 (p < .05) mRNA were constantly upregulated relative to sirtuin 1, although limited information is available regarding sirtuin 7. Among all sirtuins in mouse ovaries, sirtuin 1 was relatively and steadily downregulated. Upon sirtuin 1 overexpression in 1-day postnatal mouse ovaries via sirtuin 1-harboring adenoviruses in vitro, the emergence of primary follicles was delayed, as was the emergence of secondary follicles in 4-day postnatal ovaries. Further studies on KGN cell lines reported that interfering with sirtuin 1 expression in granulosa cell significantly affected granulosa cell proliferation and the expression of mitochondrial genes. This study presents the first systemic analysis of dynamic patterns of sirtuin family expression in early postnatal mice ovaries, laying the foundation for further studies on less discussed sirtuin subtypes, such as sirtuin 5 and sirtuin 7.

Resveratrol and SIRT1: Antiaging Cornerstones for Oocytes?

“…Depending on the amount of ATP produced, mitochondria can influence most processes within the GC cell and oocytes [ 41 , 42 ]. Silencing of the SIRT1 gene resulted in decreased expression of some of the genes associated with mitochondrial functions (Mitofusin-2 (mfn2); autophagy related 5 (Atg5) and beclin 1), suggesting a regulatory link between SIRT1 and the mitochondrion [ 43 ] and contributing to a decrease in cumulus-cell expansion [ 34 ].…”

N-Acetyl Cysteine Restores Sirtuin-6 and Decreases HMGB1 Release Following Lipopolysaccharide-Sensitized Hypoxic-Ischemic Brain Injury in Neonatal Mice

“…For instance, siRNA-mediated knockdown of SIRT6 was shown to increase the extracellular release of HMGB1 from SH-SY5Y neuronal cells following exposure to oxygen-glucose deprivation (Lee et al, 2013). Similar effects of SIRT6 knockdown were shown on other cell types (Kong et al, 2020). The reduced expression of SIRT6 in ischemic regions of adult rodent brain coincided with the cytosolic translocation of HMGB1 (Lee et al, 2013).…”

Effect of Human Amniotic Epithelial Stem Cell Transplantation on Preterm Premature Rupture of Fetal Membrane Using the Amniotic Pore Culture Technique in vitro

“…To isolate hAESCs, amniotic membranes underwent digestion twice with 0.05% trypsin-ethylenediaminetetraacetic acid (Invitrogen, CA, USA) for 30 min at 37°C. Isolated hAESCs were cultured in 100 mm diameter cell culture dishes with α-minimal essential medium (α-MEM; Welgene, Daegu, Korea) supplemented with 10% fetal bovine serum (FBS; Welgene), 1% penicillin-streptomycin (P/S; Welgene), and 10 ng/mL epidermal growth factor (EGF; R&D Systems, Minneapolis, MN, USA) [ 16 , 20 ]. All the procedures described below were performed under sterile conditions (Fig.…”

“…Isolated hAESCs were fixed with 4% paraformaldehyde for 10 min and washed three times with PBS. The experimental protocols were learned from the previous papers [20][21][22]. The cells were then incubated with antibodies against cluster of differentiation 19 (CD19), CD34, CD73, CD90 (Thy1), CD105 (endoglin), CD324 (E-cadherin), stage-spe-Gynecol Obstet Invest 2022;87:333-343 DOI: 10.1159/000527514 cific embryonic antigen 4 (SSEA4), octamer-binding transcription factor 3/4 (Oct3/4), SRY-box transcription factor 2 (Sox2), Nanog (BD Biosciences, San Jose, CA, USA), and human leukocyte antigen (HLA) class I or II (Ancell, MN, USA) for 40 min on ice after a blocking procedure.…”