Expression of Somatotropin Receptor Messenger Ribonucleic Acid in Bovine Tissues

Lucy, M.C.; Boyd, C. K.; Koenigsfeld, A.T.; Okamura, Chikako

doi:10.3168/jds.s0022-0302(98)75760-1

Cited by 57 publications

(37 citation statements)

References 22 publications

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“…Both genes were highest expressed in liver. The highest GHR and IGF-I mRNA concentrations were found in liver in accordance with previous studies related to several mammals (Lucy et al 1998, Han et al 1987, Liu et al 2000. Our measurements of GHR and IGF-I mRNA levels in cattle and yak show that different tissues need different amounts of these two growth factors for growth.…”

Section: Discussionsupporting

confidence: 78%

Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

et al. 2013

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The goals of this study were to compare the mRNA expression profiles of growth hormone recep tor (GHR) and insulin-like growth factor I (IGF-I) in various tissues of cattle and the semi-wild yak (Datong yak) and to find out whether the mRNA levels of the two genes are correlated. The mRNA levels of GHR and IGF-I in heart, lung, liver, spleen, pancreas, kidney, muscle, mammary gland and ovary of cattle and yak were investigated by using quantitative real-time po ly mer ase chain reaction (PCR). The experiments showed that the transcript levels of the two genes were sig nif i cant ly higher in liver (P<0.05) than in the other tissues for both species and that IGF-I levels varied more among tissues (P<0.01) than did GHR levels. The GHR tran script level in pancreas was higher in yak (P<0.05) than in cattle. There was no statistically sig nif i cant difference in IGF-I tran script levels among all the tissues of both bovine groups. Growth hormone receptor and IGF-I transcript levels were positively correlated in mammary gland (P<0.01), lung (P<0.05) and muscle (P<0.05) in yak, negatively correlated in cattle heart (P<0.05) and not correlated in the other tissues. The results indicate that the two genes are reg u lated differently in various tissues under normal physiological conditions in these two bovine species.

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Section: Discussionsupporting

confidence: 78%

Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

et al. 2013

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“…The presence of mRNA for GH receptors in the hypothalamus and the pituitary gland [30,31] has raised the possibility that GH plays a role in the link between nutrition and gonadotrophin secretion [32]. In ruminants, energy balance affects plasma concentrations of GH under a wide variety of experimental conditions [33] but, in male sheep, it does not seem to be involved in the stimulation of GnRH secretion because an increase in nutrition induces a decrease in plasma GH concentrations [13].…”

Section: Growth Hormonementioning

confidence: 99%

Dynamic and integrative aspects of the regulation of reproduction by metabolic status in male sheep

2006

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-Change in metabolic status, defined as a change in the availability of nutrients and energy to the tissues, is a powerful regulator of the reproductive function in small ruminants, especially in genotypes that are not strongly responsive to photoperiod such as the Merino sheep. In this paper, the dynamics of the response of the reproductive axis to changes in metabolic status are reviewed in the light of recent studies. The nature and the roles of the various components of the pathways linking metabolic status to reproduction are considered: nutrients and metabolites, the endocrine system, and the nervous system. We discuss the role of leptin and insulin in detail because of the central role of these two hormones in both the early gonadotrophin response to increase in nutrition and the long-term response of the testis to dietary stimulation. The possible roles of recently identified peptides, such as ghrelin and kisspeptin, are also considered as we develop a general hypothesis that encompasses the different levels of integration necessary to explain the complex interactions between reproductive function and metabolic status, and the possible existence of a "metabolic memory" in this interaction.nutrition / GnRH / LH / leptin / insulin / testis

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“…Its level increased slightly with advancing gestation. In the bovine foetus, Lucy et al [18] reported the presence of GHR mRNA in several tissues, including the muscle and liver. In addition, both GHR mRNA and GHR protein have been detected in bovine embryos [19].…”

Section: Introductionmentioning

confidence: 99%

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

Listrat

Hocquette²,

Picard³

et al. 2005

Reprod. Nutr. Dev.

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-The expression of the growth hormone receptor (GHR) gene was investigated in semitendinosus muscle during bovine foetal development in both normal and double-muscled Charolais foetuses which differ with respect to muscle development. Northern-blot analysis of foetal muscle RNA preparations with a GHR cDNA probe identified the 4.5 kb GHR mRNA as early as 130 days post-conception. In double-muscled animals, the expression of GHR mRNA increased from 130 to 210 days of gestation while it stayed stable in normal ones. It was significantly higher (P < 0.05) in double-muscled foetuses compared to normal ones from the second third of gestation. Northern-blot analysis of foetal muscle RNA preparations from both genotypes with a β-actin cDNA probe, revealed lower β-actin gene expression in double-muscled foetuses than in normal ones, suggesting a delay in the differentiation of muscle cells. In situ hybridisation revealed the localisation of specific GHR mRNA in muscle cells at all gestation stages analysed (130, 170, 210 days postconception) but not in connective tissue surrounding the muscle cells. At the adult stage, the hybridisation signal was also very high and observed in muscle cells only. These results show the ontogeny of GHR mRNA in bovine muscle and demonstrate a difference between normal and doublemuscled animals. muscle / bovine / receptor / growth hormone / in situ hybridisation

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Expression of Somatotropin Receptor Messenger Ribonucleic Acid in Bovine Tissues

Cited by 57 publications

References 22 publications

Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

Expression profiles of growth hormone receptor and insulin-like growth factor I in cattle and yak tissues revealed by quantitative real-time PCR

Dynamic and integrative aspects of the regulation of reproduction by metabolic status in male sheep

Growth hormone receptor gene expression in the skeletal muscle of normal and double-muscled bovines during foetal development

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