Expression of stress-responsive ubiquitin genes in potato tubers

Abstract

The stress-induced expression of four different ubiquitin-encoding cDNAs was characterized in potato tuber tissue. The four clones exhibited differences in both structure and expression. The first cDNA encoded a single ubiquitin unit fused to an 80 amino acid ribosomal extension protein identical to the extension protein from tomato. Accumulation of the fusion transcript was induced by injury or ethylene, but not by heat shock. The three remaining ubiquitin cDNAs encoded polyubiquitins with 6 to 7 ubiquitin repeats. The first polyubiquitin gene was induced by injury, heat, or ethylene treatments. The second was induced also by injury or heat, with limited ethylene-dependent accumulation of transcript. Transcript levels of the third polyubiquitin gene were highest in control tubers and decreased markedly with injury, heat shock, or ethylene treatment. The data demonstrate the independent regulation of the different members of the ubiquitin gene family in response to stress and exogenous ethylene.

Identification of ABA-responsive genes in rice shoots via cDNA macroarray

“…The possible multi-regulated clones encoded putative cytochrome P450, MADS box transcription factor, Proline-rich protein precursor, seed maturation protein and so on. Together with the results that ethylene-response protein was regulated by ABA (Fig 2, clone Xd439, AJ437339), although the detailed analysis on the regulation by both hormones is still required, these may indicate the complicated interIdentification of ABA-responsive genes in rice [47][48][49]. Although there was no report about the connection of auxin and ubiquitin, but the promoter of GLP3b gene has a single putative auxin-response element, which was physically linked to the polyubiquitin 4 gene [50].…”

Differential Accumulation of Sunflower Tetraubiquitin mRNAs during Zygotic Embryogenesis and Developmental Regulation of Their Heat-Shock Response

“…Polyubiquitin genes have been isolated from different plants (Causing and Barkardottir, 1986;Burke et al, 1988;Christensen and Quail, 1989;Jamet et al, 1990;Genschik et al, 1992;Garbarino et al, 1992;Reynolds and Hooley, 1992), including sunflower (Binet et al, 1989(Binet et al, , 1991. The nucleotide sequences of these genes are highly conserved, mainly in their coding regions and in a characteristic 5' flanking intron (Binet et al, 1991, and refs.…”

“…therein). Sequence divergence in their 3' flanking regions (Joshi et al, 1991) and the expression of different sized messengers (Causing and Barkardottir, 1986;Burke et al, 1988;Genschik et al, 1992) have allowed detection of the differential accumulation of polyubiquitin mRNAs, mainly in response to heat shock (Christensen and Quail, 1989;Binet et al, 1991;Christensen et al, 1992;Genschik et al, 1992) and other stress treatments (Garbarino et al, 1992;Genschik et al, 1992) but also during leaf (Causing and Barkardottir, 1986;Burke et al, 1988) or flower (Causing and Barkardottir, 1986;Binet et al, 1989) development and in cell dedifferentiation (Jamet et al, 1990). The finding of multiple, differentially regulated polyubiquitin genes in Arabidopsis and in all plants studied so far has suggested (Burke et al, 1988) that evolutionary divergence of their regulation provides basic, conserved functions (reviewed by Monia et al, 1990;Finley and Chau, 1991;Hershko and Ciechanover, 1992;Jentsch, 1992;Vierstra, 1993) in specific temporal or spatial environments.…”

“…This, together with observation of differential regulation of severa1 plant ubiquitin genes, has suggested that their multiplicity and diversity reflect an evolutionary divergence of their gene regulation to cope with different specific tasks, such as functions related to cell proliferation and dedifferentiation (Gausing and Barkardottir, 1986;Jamet et al, 1990;Genschik et al, 1992); stress, light, or hormone responses (Burke et al, 1988;Binet et al, 1991;Hoffman et al, 1991;Garbarino et al, 1992;Genschik et al, 1992;Reynolds and Hooley, 1992); and organ (flower, leaf, etc.) development (Gausing and Barkardottir, 1986;Burke et al, 1988;Hoffman et al, 1991;Garbarino et al, 1992). The differential regulation of tetraubiquitin mRNAs during seed maturation and detection of putative, seed-specific, protein-ubiquitin conjugates reported in this study suggest that distinct ubiquitin genes could perform specific functions during plant zygotic embryogenesis.…”

Crop Improvement through Modification of the Plant's Own Genome

“…AY566555) flanked by HindIII and SpeI sites was inserted into the corresponding sites of the resulting plasmid, and an expression cassette comprising the Agrobacterium ipt gene preceded by the Ubi-3 promoter (Garbarino and Belknap, 1994) and followed by the Ubi-3 terminator was introduced as a 2.6-kb SacII fragment. The P-DNA vector pSIM108 contains the nptII gene, driven by the Ubi-7 promoter (Garbarino et al, 1992) and followed by the terminator of the yeast (Saccharomyces cerevisiae) alcohol dehydrogenase gene (GenBank accession no. V01292), positioned within the P-DNA region.…”