Expression of survivin and XIAP for DMBA-induced hamster buccal pouch squamous cell carcinogenesis is associated with p53 accumulation

Chemopreventive effect of Toona sinensis leaf extract on 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch squamous cell carcinogenesis

“…The strongest expressions were observed in malignant epithelium. The trend observed in the present study was consistent with the results of our previous studies of survivin, XIAP, and iNOS (Chen & Lin, 2000;Hsue et al, 2008). Comparing the labeling index (LI) of PCNA in our study with the results of studies by Grawish, Zaher, Gaafar, and Nasif (2010), Kobayashi et al (1995), and Tsuji et al (1992), there were minor differences in the PCNA LI value, but the trend and the conclusion were the same.…”

“…Kohno et al (2002) reported a similar change of the AI of the TUNEL assay. Survivin and XIAP are also associated with the regulation of apoptosis (Hsue et al, 2008). Increasing expressions of survivin and XIAP will inhibit apoptotic activity.…”

“…No. G7341 Promega Corporation, WI, USA), and their specificity has been established in previous studies (Baldasquin-Caceres, Gomez-Garcia, López-Jornet, Castillo-Sanchez, & Vicente-Ortega, 2014; Chen & Lin, 2000;Hsue, Chen, & Lin, 2008;Jiang & Wang, 2004;Kockx, Muhring, Knaapen, & de Meyer, 1998;Nishimura et al, 2004). The tissue sections were deparafinized in xylene 3 times and rehydrated in a graded series of ethanol (absolute, 95%, 70%) and distilled water.…”

Expression of inhibitors of apoptosis family protein in 7,12‐dimethylbenz[a]anthracene‐induced hamster buccal‐pouch squamous‐cell carcinogenesis is associated with mutant p53 accumulation and epigenetic changes

“…Only two IAP family members, namely survivin and XIAP proteins, have been reported to be overexpressed in DMBA‐induced hamster buccal‐pouch carcinogenesis recently in our laboratory (Chen et al. 2005a; Hsue et al. 2008).…”

“…The p53 tumour‐suppressor gene is implicated in cell cycle checkpoint mechanisms, inhibiting cell cycle progression and inducing apoptosis in reaction to DNA damage (Gottlieb & Oren 1998). Given that an association of p53 expression and survivin, as well as XIAP, has been previously noted in hamster buccal‐pouch carcinogenesis (Hsue et al. 2007), the relationship of other members of the IAP family and p53 is still an interesting area for study.…”