Expression of T-helper-associated cytokines in the serum of pituitary adenoma patients preoperatively and postperatively

Qiu, Lubin; Yang, Jianming; Wang, Haijun; Zhu, Yongjun; Wang, Yatang; Wu, Qian

doi:10.1016/j.mehy.2013.03.011

Cited by 14 publications

(11 citation statements)

References 28 publications

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“…We also investigate the abundance of immune cells in NFPA using the CIBERSORT algorithm, which showed that B cells naive, T cells CD4 naive, and Macrophages M1 were significantly different between the CS invasion and CS non-invasion group. Previous studies have shown that TMErelated factors are associated with invasive pituitary adenomas, such as the number of M2-macrophages, the number of inflammatory cells, and increased expression of PD-L1 (Qiu et al, 2013;Sato et al, 2019). In addition, Kaplan-Meier survival analysis showed that a lower immune/stromal score was associated with better RFS.…”

Section: Discussionmentioning

confidence: 90%

Screening and Identification of Key Microenvironment-Related Genes in Non-functioning Pituitary Adenoma

Guo

Fang²,

Liu³

et al. 2021

Front. Genet.

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PurposeNon-functioning pituitary adenoma (NFPA) is a very common type of intracranial tumor, which can be locally invasive and can have a high recurrence rate. The tumor microenvironment (TME) shows a high correlation with tumor pathogenesis and prognosis. The current study aimed to identify microenvironment-related genes in NFPAs and assess their prognostic value.Methods73 NFPA tumor samples were collected from Beijing Tiantan Hospital and transcriptional expression profiles were obtained through microarray analysis. The immune and stromal scores of each sample were calculated through the ESTIMATE algorithm, and the patients were divided into high and low immune/stromal score groups. Intersection differentially expressed genes (DEGs) were then obtained to construct a protein–protein interaction (PPI) network. Potential functions and pathways of intersection DEGs were then analyzed through Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes. The prognostic value of these genes was evaluated. The quantitative real-time polymerase chain reaction in another set of NFPA samples was used to confirm the credibility of the bioinformatics analysis.ResultsThe immune/stromal scores were significantly correlated with cavernous sinus (CS) invasion. The Kaplan–Meier curve indicated that the high immune score group was significantly related to poor recurrence-free survival. We identified 497 intersection DEGs based on the high vs. low immune/stromal score groups. Function enrichment analyses of 497 DEGs and hub genes from the PPI network showed that these genes are mainly involved in the immune/inflammatory response, T cell activation, and the phosphatidylinositol 3 kinase-protein kinase B signaling pathway. Among the intersection DEGs, 88 genes were further verified as significantly expressed between the CS invasive group and the non-invasive group, and five genes were highly associated with NFPA prognosis.ConclusionWe screened out a series of critical genes associated with the TME in NFPAs. These genes may play a fundamental role in the development and prognosis of NFPA and may yield new therapeutic targets.

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Section: Discussionmentioning

confidence: 90%

Screening and Identification of Key Microenvironment-Related Genes in Non-functioning Pituitary Adenoma

Guo

Fang²,

Liu³

et al. 2021

Front. Genet.

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“…Measuring plasma cytokines concentrations to estimate Th cell balance may be inaccurate because all IFN-gamma and IL-4 present in plasma are not derived solely from Th1 and Th2 cells. Although we did not perform flow cytometry to measure intracellular cytokines to accurately estimate Th cell balance, several other studies have been reported to describe the Th cell balance based on the analysis of whole blood sample by ELISA [ 22 , 23 ]. In particular, Silva et al [ 24 ] asserted that whole blood analysis and mono-nuclear cells techniques using peripheral blood samples are suitable for the study of cytokines production.…”

Section: Discussionmentioning

confidence: 99%

Immunomodulatory effects of intraoperative dexmedetomidine on T helper 1, T helper 2, T helper 17 and regulatory T cells cytokine levels and their balance: a prospective, randomised, double-blind, dose-response clinical study

et al. 2018

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BackgroundThe ratio of T helper 1 (Th1) to T helper 2 (Th2) as well as T helper 17 (Th17) to regulatory T cells (Treg) represents the state and direction of immune response. Recent studies demonstrated that dexmedetomidine reduced the secretion of inflammatory cytokines. We performed this study to investigate the effect of different doses of intraoperative dexmedetomidine on the expression of Th1, Th2, T17 and Treg cytokines and their ratios.MethodsSeventy-five patients undergoing laparoscopic cholecystectomy were randomly separated into one of three groups: the full dose group (n = 25), in which dexmedetomidine was infused with a 1.0 μg/kg loading followed by an infusion of 0.5 μg/kg/min after anaesthetic induction, or the half dose group (n = 26), in which the dose was half of that of full dose group, or the saline group (n = 24) which was control. T cell cytokines were quantified by sandwich enzyme-linked immunoassay for blood samples taken after anaesthetic induction (T0), at the end of surgery (T1), and 60 min after surgery (T2). IFN-gamma/IL-4 and IL-17/IL-10, which represent the ratio of Th1/Th2 and Th17/Treg cytokines, respectively, were calculated as indices of immune cell levels based upon serum cytokines levels in place of direct measurements. C-reactive protein (CRP) concentrations were measured on the next day following surgery.ResultsThe full dose group was associated with higher ratios of IFN-gamma/IL-4 than those of half dose group and control [10.1 vs. 1.9 at T1 (P = 0.041) compared with half dose group, and 10.1 vs. 0.2 at T1 (P = 0.031), 7.4 vs. 0.1 at T2 (P = 0.025) compared with control]. IL-17/IL-10 ratios were higher in the full dose group than those in control [4.2 vs. 0.6 at T1 (P = 0.013), 3.0 vs. 0.3 at T2 (P = 0.011)]. The CRP levels were lower in the dexmedetomidine-treated groups in a dose-dependent manner.ConclusionsDexmedetomidine exhibits immunomodulatory effects, shifting the Th1/Th2 and T17/Treg cytokine balance toward Th1 and T17, respectively, in a dose-dependent pattern in patients with surgical and anaesthetic stress.Trial registrationClinical Research Information Service, Republic of Korea (CRIS); KCT0000503; Registration date: Aug 13, 2012.

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“…To our knowledge, only one study has explored IL-17A concentration in the serum of patients with PA, but no studies have investigated the association between serum IL-17 and PA growth type. Qiu et al [ 42 , 43 ] have investigated IL-17A serum concentration level of 76 PA patients, and they have observed that serum level of IL-17A was significantly higher in patients with invasive PAs than in the group with noninvasive PAs. Serum level of IL-17A was found to be 95.46 ± 34.09 pg/mL in invasive PA patients, 56.26 ± 14.03 pg/mL in noninvasive PA patients, and 23.58 ± 6.55 pg/mL in the control group.…”

Section: Discussionmentioning

confidence: 99%

“…It has been found that IL-17A plays an important role in the development of various tumours, for example, breast cancer, hepatocellular carcinoma, and cervical cancer [ 36 – 39 ]. However, some studies have revealed that IL-17A inhibits the development of tumours [ 40 , 41 ] and there is only one survey that analyses the IL-17A impact on the PA growth [ 42 , 43 ]. We also hypothesized that the development of PA can be influenced by the concentration of IL-17 A.…”

Section: Introductionmentioning

confidence: 99%

Association of Ki-67 Labelling Index and IL-17A with Pituitary Adenoma

Glebauskiene

Liutkevičienė

Vilkeviciute

et al. 2018

BioMed Research International

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The aim of the present study was to determine if the Ki-67 labelling index reflects invasiveness of pituitary adenoma and to evaluate IL-17A concentration in blood serum of pituitary adenoma patients. The study was conducted in the Hospital of Lithuanian University of Health Sciences. All pituitary adenomas were analysed based on magnetic resonance imaging findings. The suprasellar extension and sphenoid sinus invasion by pituitary adenoma were classified according to Hardy classification modified by Wilson. Knosp classification system was used to quantify the invasion of the cavernous sinus. The Ki-67 labelling index was obtained by immunohistochemical analysis with the monoclonal antibody, and serum levels of IL-17A were determined by enzyme-linked immunosorbent assay (ELISA). Sixty-nine PA tissue samples were investigated. Serum levels of IL–17A were determined in 60 patients with PA and 64 control subjects. Analysis revealed statistically significantly higher Ki-67 labelling index in invasive compared to noninvasive pituitary adenomas. Median serum IL-17A level was higher in the pituitary adenoma patients than in the control group. Conclusion. IL-17A might be a significant marker for patients with pituitary adenoma and Ki-67 labelling index in case of invasive pituitary adenomas.

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Expression of T-helper-associated cytokines in the serum of pituitary adenoma patients preoperatively and postperatively

Cited by 14 publications

References 28 publications

Screening and Identification of Key Microenvironment-Related Genes in Non-functioning Pituitary Adenoma

Screening and Identification of Key Microenvironment-Related Genes in Non-functioning Pituitary Adenoma

Immunomodulatory effects of intraoperative dexmedetomidine on T helper 1, T helper 2, T helper 17 and regulatory T cells cytokine levels and their balance: a prospective, randomised, double-blind, dose-response clinical study

Association of Ki-67 Labelling Index and IL-17A with Pituitary Adenoma

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