Expression of telomerase mRNA component (hTR) in transitional cell carcinoma of the upper urinary tract

Nakanishi, Kuniaki; Kawai, Toshiaki; Hiroi, Miki; Kumaki, Fumiyuki; Torikata, Chikao; Aurues, Takashi; Ikeda, Tomosumi

doi:10.1002/(sici)1097-0142(19991115)86:10<2109::aid-cncr32>3.0.co;2-6

Cited by 27 publications

(12 citation statements)

References 28 publications

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“…Those authors suggested that cells expressing high levels of telomerase have a selective growth advantage because of the improved stability of chromosomes with restored telomeres. In TCC of the urinary tract, a relationship between telomerase activity and progression (as defined by stage and grade) has not been found in previous studies (11,15,22). With regard to the relationship between hTERT expression and clinicopathologic findings in TCC of the bladder, only one report (dealing with 35 cases) has found that increases in pathologic grade and clinical stage correlated with an increased hTERT expression (assessed using RT-PCR) (17).…”

Section: Discussionmentioning

confidence: 97%

“…Several investigators have demonstrated a close relationship between hTERT expression and telomerase activity, indicating that transcription of hTERT may be involved in the mechanism underlying telomerase regulation (6). In contrast, hTERC was found to be expressed in the normal urothelium of the urinary bladder by Ito et al (19), using RT-PCR, and in that of the upper urinary tract by us (22), using in situ hybridization. Furthermore, in the present study, we demonstrated a close relationship between hTERT scores and hTERC in TCC-UUT.…”

Section: Discussionmentioning

confidence: 98%

“…In situ hybridization was performed essentially as previously described (22). Briefly, sections were treated with 0.2 N HCl for 20 min, then incubated in 2ϫ SSC for 10 min at 37°C, and incubated in 5 g/mL proteinase k for 10 min at 37°C.…”

Section: Methodsmentioning

confidence: 99%

“…The hTERC expression was evaluated using in situ hybridization; the technique used and the results obtained in these same patients have been reported elsewhere (22).…”

Section: Methodsmentioning

confidence: 99%

“…For the analysis of PCNA, and on the basis of the immunoreaction in at least 1000 tumor cells, the percentage of nuclei with a positive immunoreaction (PCNA index) was determined as previously described (22). The PCNA index was classified as high if it was Ն69%, a figure representing the median value for the carcinomas.…”

Section: Methodsmentioning

confidence: 99%

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Expression of Telomerase Catalytic Subunit (hTERT) mRNA Does Not Predict Survival in Patients with Transitional Cell Carcinoma of the Upper Urinary Tract

et al. 2001

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Telomerase is a ribonucleoprotein enzyme that synthesizes telomeric repeats onto chromosomal ends using a segment of its RNA component as a template. Its activity has become an established indicator of the diagnosis, biological behavior, and prognosis of several tumors. However, few studies have investigated the diagnostic and prognostic importance of the expression of telomerase catalytic subunit (hTERT) mRNA in transitional cell carcinoma of the upper urinary tract (TCC-UUT). We investigated the expression of hTERT mRNA using in situ hybridization in 125 cases of TCC-UUT, and also its relation with the expression of telomerase RNA component (hTERC), proliferating cell nuclear antigen (PCNA) immunoreactivity, clinicopathologic parameters, and clinical outcome. A positive expression of hTERT mRNA was recognized in 93.6% of the samples and was apparent within the cytoplasm of tumor cells. In the normal urothelium examined in a few cases, its expression was barely detected. hTERT mRNA scores showed a significant association with hTERC score. However, no relationship was found between the expression of hTERT mRNA and clinicopathologic findings, PCNA index, or prognosis. These results suggest that the expression of hTERT mRNA does not predict prognosis in TCC-UUT. KEY WORDS: Telomerase catalytic subunit (hTERT), Transitional cell carcinoma, Upper urinary tract.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

Section: Methodsmentioning

confidence: 99%

“…The hTERC expression was evaluated using in situ hybridization; the technique used and the results obtained in these same patients have been reported elsewhere (22).…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Expression of Telomerase Catalytic Subunit (hTERT) mRNA Does Not Predict Survival in Patients with Transitional Cell Carcinoma of the Upper Urinary Tract

et al. 2001

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PCR andin situ hybridization studies of telomerase subunits in human non-neoplastic livers

et al. 2001

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Telomerase, a ribonucleoprotein enzyme associated with cellular immortality, consists of human telomerase RNA component (hTERC), human telomerase protein 1 (hTEP1), and human telomerase reverse transcriptase (hTERT). In this study, the expression of these subunits was examined in non-neoplastic livers [13 cases of chronic viral hepatitis (CVH), 16 of primary biliary cirrhosis (PBC), two of primary sclerosing cholangitis, and six normal livers], using the reverse transcription-polymerase chain reaction (RT-PCR), nested PCR, and in situ hybridization (ISH). Six hepatocellular carcinoma (HCC) cases and one colonic cancer were used as positive controls. Telomeric repeat amplification protocol (TRAP) assay disclosed distinct telomerase activity in all positive controls and weak telomerase activity in non-neoplastic livers in 4 of 13 CVH cases and 5 of 16 PBC cases. By RT- and nested PCR, both hTERC and hTEP1 mRNA were detectable in all non-neoplastic liver tissues; ISH revealed hTERC and hTEP1 mRNA in the periportal and periseptal hepatocytes and inflammatory mononuclear cells in those cases examined. ISH revealed hTERT mRNA only in a few infiltrating mononuclear cells in 3 of 13 CVH and 2 of 16 PBC livers and these five cases were also positive by TRAP assay. In four of these five cases, hTERT mRNA was also detectable by nested PCR, suggesting that hTERT mRNA in the non-neoplastic liver is expressed by infiltrating mononuclear cells. Biliary epithelial cells were totally negative for these human telomerase subunits. Three subunits were constantly detected in all positive controls by ISH as well as by RT- and nested PCR. The finding that hTERC and hTEP1 mRNA, but not hTERT mRNA, were detectable in the non-neoplastic hepatocytes suggests that telomerase is present but not activated and that additional factor(s) are necessary for the expression of hTERT mRNA in the hepatocytes, along with immortalization and neoplastic transformation.

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Expressions of human telomerase mRNA component (hTERC) and telomerase reverse transcriptase (hTERT) mRNA in effusion cytology

Hiroi

Nakanishi

Kawai

2003

Diagnostic Cytopathology

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Telomerase is a ribonucleoprotein enzyme that synthesizes telomeric DNA onto chromosomal ends. Its activity has been reported to be a novel diagnostic marker of malignant potential. However, no study has investigated the diagnostic importance of the expressions of human telomerase mRNA component (hTERC) and telomerase reverse transcriptase (hTERT) mRNA in effusion cytology. We investigated the expressions of hTERC and hTERT using in situ hybridization in 20 malignant cytologic specimens from 20 patients with adenocarcinomas disseminating into the pleura or peritoneum, and 16 benign cytologic specimens in pleural or peritoneal fluid (including reactive mesothelial cells) from 16 patients with non-neoplastic diseases. Expressions of hTERC and hTERT mRNA were detected in 95% and 90% of the 20 malignant cytology specimens, respectively, and were apparent within the cytoplasm of malignant cells. However, no expression was detected in reactive mesothelial cells in the 16 benign cytologic specimens. The expressions of hTERC and hTERT mRNA would appear to be a useful addition to cytology examination for diagnosis based on effusion cytology.

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Expression of telomerase mRNA component (hTR) in transitional cell carcinoma of the upper urinary tract

Cited by 27 publications

References 28 publications

Expression of Telomerase Catalytic Subunit (hTERT) mRNA Does Not Predict Survival in Patients with Transitional Cell Carcinoma of the Upper Urinary Tract

Expression of Telomerase Catalytic Subunit (hTERT) mRNA Does Not Predict Survival in Patients with Transitional Cell Carcinoma of the Upper Urinary Tract

PCR andin situ hybridization studies of telomerase subunits in human non-neoplastic livers

Expressions of human telomerase mRNA component (hTERC) and telomerase reverse transcriptase (hTERT) mRNA in effusion cytology

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