Expression of tetraspanins in peripheral blood leukocytes: a comparison between normal and infectious conditions

Abstract

The role of tetraspanins is undefined, despite their detection in diverse cell types and functions. This study addresses the characterization of tetraspanin expression levels in normal peripheral blood leukocytes (PBL) and in patients with bacterial infection. Membranal and cytoplasmic expression of CD9, CD53, CD63, CD81, CD82 and CD151 in polymorphonuclears (PMN), monocytes, B and T lymphocytes was assessed using flow cytometry. Results suggested that for normal PBL, PMN are distinguished by dominant cytoplasmic CD63; monocytes and B cells prevailingly express CD53; CD82 is primarily expressed on T-cell membranes. However, a major trend of downregulation was demonstrated for the examined tetraspanins, except CD63, in all patients' PBL subtypes. Therefore, tetraspanin modulation in infections may be attributed to elevated leukocyte motility in immune reactions and this is compatible with the previous publications of tetraspanins as metastasis suppressors. This work represents the first comprehensive baseline of tetraspanin expression in normal PBL and in infectious disorders.

Specific Targeting of Human Inflamed Endothelium and In Situ Vascular Tissue Transfection by the Use of Ultrasound Contrast Agents

“…This role suggests that localized release of blocking antibodies against CD9 might have potential as a general and more effective antiinflammatory therapy than the single inhibition of one of these adhesion molecules. However, CD9 is not only expressed by endothelial cells but also by a number of blood cell subsets (including platelets, neutrophils, and monocytes) (7,20). Therefore, the administration of CD9 blockers would require a localized release in inflammatory foci, which could be accomplished by their combination with antibodies against any inflammatory marker in the same MB.…”

Effect of prenatal exposure to the endocrine disruptor bisphenol A on mammary gland morphology and gene expression signature

“…Interestingly, in both experimental conditions (low and high doses), the age with the maximal number of modulated genes presented an important cluster related to immune response, including Ctse (Sealy et al 1996), Cd53 (Tohami et al 2004), Cd3d (Fischer et al 2005), or secretory leukocyte peptidase inhibitor (Slpi; King et al 2003). In addition, the cytoskeletal protein Coronin-1A (Coro1a), also found up-modulated by effect of low and high doses at 50 and 100 days respectively, has been reported to play a role in T cell differentiation/activation events (Nal et al 2004).…”

cDNA microarray analysis reveals novel candidate genes expressed in human peripheral blood following exhaustive exercise

“…Integrin-␣x (CD11c) and CD81 mRNA were both downregulated significantly in this study. This cannot be attributed to cellular shifts, since CD11c is strongly or moderately expressed in monocytes, macrophages, NK cells and granulocytes, while it is low in T and B cells (26), and CD81 is expressed on polymorphonuclears (PMN), monocytes, and B and T lymphocytes (59). The lymphocytes are the minor population and therefore can hardly explain the strong 50% overall downregulation of gene expression.…”