Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana

Chao, Yuehui; Yang, Qingchuan; Sun, Yan; Gruber, Margaret Y.; Qin, Z. H.

doi:10.1016/j.plantsci.2013.10.012

Cited by 32 publications

(21 citation statements)

References 45 publications

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“…Two downregulated genes (GRMZM2G105224 and GRMZM5G801627) contained C 2 H 2 and CCCH zinc fingers. Similar zinc finger proteins have been reported play roles in repressing flowering time (Weingartner et al, 2011 ; Chao et al, 2014 ). In addition, C 2 H 2 , bHLH, and NAC domain-containing proteins have already been reported to be important for responses to various abiotic stresses (Shinozaki and Yamaguchi-Shinozaki, 2007 ; Marino et al, 2008 ; Golldack et al, 2014 ).…”

Section: Discussionsupporting

confidence: 70%

“…Genes such as FLOWERING LOCUS C (FLC), CONSTANS (CO), GIGANTEA (GI), and SUPPRESSOR of OVEREXPRESSION of CONSTANS1 (SOC1) were known to regulate flowering time (Kim et al, 2006 ; Buckler et al, 2009 ; Jung and Müller, 2009 ; Kong et al, 2010 ; Endo-Higashi and Izawa, 2011 ; Hung et al, 2012 ; Ito et al, 2012 ; Li et al, 2016 ). Expression of these genes controlled by photoperiod, light sensing, circadian, and various stress (Johnson et al, 1994b ; Datta, 2006 ; Fornara et al, 2009 ; Jung and Müller, 2009 ; Meng et al, 2011 ; Hung et al, 2012 ; Coelho et al, 2013 ; Chao et al, 2014 ). Similarly, drought known make changes of ASI, therefore drought maybe affects the flowering time genes.…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome Analysis of Flowering Time Genes under Drought Stress in Maize Leaves

et al. 2017

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Flowering time is an important factor determining yield and seed quality in maize. A change in flowering time is a strategy used to survive abiotic stresses. Among abiotic stresses, drought can increase anthesis-silking intervals (ASI), resulting in negative effects on maize yield. We have analyzed the correlation between flowering time and drought stress using RNA-seq and bioinformatics tools. Our results identified a total of 619 genes and 126 transcripts whose expression was altered by drought stress in the maize B73 leaves under short-day condition. Among drought responsive genes, we also identified 20 genes involved in flowering times. Gene Ontology (GO) enrichment analysis was used to predict the functions of the drought-responsive genes and transcripts. GO categories related to flowering time included reproduction, flower development, pollen–pistil interaction, and post-embryonic development. Transcript levels of several genes that have previously been shown to affect flowering time, such as PRR37, transcription factor HY5, and CONSTANS, were significantly altered by drought conditions. Furthermore, we also identified several drought-responsive transcripts containing C2H2 zinc finger, CCCH, and NAC domains, which are frequently involved in transcriptional regulation and may thus have potential to alter gene expression programs to change maize flowering time. Overall, our results provide a genome-wide analysis of differentially expressed genes (DEGs), novel transcripts, and isoform variants expressed during the reproductive stage of maize plants subjected to drought stress and short-day condition. Further characterization of the drought-responsive transcripts identified in this study has the potential to advance our understanding of the mechanisms that regulate flowering time under drought stress.

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Section: Discussionsupporting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of Flowering Time Genes under Drought Stress in Maize Leaves

et al. 2017

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“…However, further studies are required to characterize the gymnosperm-specific genes involved in the female transition phase. Recent studies have shown that zinc finger CCCH domain-containing proteins regulate flowering time and play significant roles in developing floral tissue (Colasanti et al, 2006 ; Chao et al, 2014 ). In addition, the CONSTANS family of transcription factors, some of which play important roles in photoperiod-induced floral induction, have a unique combination of zinc fingers.…”

Section: Discussionmentioning

confidence: 99%

Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways

Zhao

Liang

et al. 2015

Front. Plant Sci.

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Metasequoia glyptostroboides is a famous redwood tree of ecological and economic importance, and requires more than 20 years of juvenile-to-adult transition before producing female and male cones. Previously, we induced reproductive buds using a hormone solution in juvenile Metasequoia trees as young as 5-to-7 years old. In the current study, hormone-treated shoots found in female and male buds were used to identify candidate genes involved in reproductive bud transition in Metasequoia. Samples from hormone-treated cone reproductive shoots and naturally occurring non-cone setting shoots were analyzed using 24 digital gene expression (DGE) tag profiles using Illumina, generating a total of 69,520 putative transcripts. Next, 32 differentially and specifically expressed transcripts were determined using quantitative real-time polymerase chain reaction, including the upregulation of MADS-box transcription factors involved in male bud transition and flowering time control proteins involved in female bud transition. These differentially expressed transcripts were associated with 243 KEGG pathways. Among the significantly changed pathways, sugar pathways were mediated by hormone signals during the vegetative-to-reproductive phase transition, including glycolysis/gluconeogenesis and sucrose and starch metabolism pathways. Key enzymes were identified in these pathways, including alcohol dehydrogenase (NAD) and glutathione dehydrogenase for the glycolysis/gluconeogenesis pathway, and glucanphosphorylase for sucrose and starch metabolism pathways. Our results increase our understanding of the reproductive bud transition in gymnosperms. In addition, these studies on hormone-mediated sugar pathways increase our understanding of the relationship between sugar and hormone signaling during female and male bud initiation in Metasequoia.

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“…Plant C3H-type zinc finger proteins are involved in multiple developmental processes, including embryo development [ 48 ], secondary xylem formation [ 49 ], seed germination [ 25 ], leaf senescence [ 22 , 23 ], and flower development [ 14 , 15 , 28 , 31 ]. In this study, CpCZF1 and CpCZF2 , both caused alterations on the stamens when overexpressed in the Arabidopsis plant, respectively, which indicated that they may function in regulating flower development, especially stamen identity specification in transgenic Arabidopsis .…”

Section: Discussionmentioning

confidence: 99%

“…GhZFP1, a novel C3H-type zinc finger protein from cotton, enhances salt stress tolerance and fungal disease resistance in tobacco [ 30 ]. MsZFN from alfalfa delays the flowering time by repressing the flowering genes in transgenic Arabidopsis [ 31 ].…”

Section: Introductionmentioning

confidence: 99%

Two C3H Type Zinc Finger Protein Genes, CpCZF1 and CpCZF2, from Chimonanthus praecox Affect Stamen Development in Arabidopsis

Liu

Huang

et al. 2017

Genes

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Wintersweet (Chimonanthus praecox) is a popular garden plant because of its flowering time, sweet fragrance, and ornamental value. However, research into the molecular mechanism that regulates flower development in wintersweet is still limited. In this study, we sought to investigate the molecular characteristics, expression patterns, and potential functions of two C3H-type zinc finger (CZF) protein genes, CpCZF1 and CpCZF2, which were isolated from the wintersweet flowers based on the flower developmental transcriptome database. CpCZF1 and CpCZF2 were more highly expressed in flower organs than in vegetative tissues, and during the flower development, their expression profiles were associated with flower primordial differentiation, especially that of petal and stamen primordial differentiation. Overexpression of either CpCZF1 or CpCZF2 caused alterations on stamens in transgenic Arabidopsis. The expression levels of the stamen identity-related genes, such as AGAMOUS (AG), PISTILLATA (PI), SEPALLATA1 (SEP1), SEPALLATA2 (SEP2), SEPALLATA3 (SEP3), APETALA1 (AP1), APETALA2 (AP2), and boundary gene RABBIT EAR (RBE) were significantly up-regulated in CpCZF1 overexpression lines. Additionally, the transcripts of AG, PI, APETALA3 SEP1-3, AP1, and RBE were markedly increased in CpCZF2 overexpressed plant inflorescences. Moreover, CpCZF1 and CpCZF2 could interact with each other by using yeast two-hybrid and bimolecular fluorescence complementation assays. Our results suggest that CpCZF1 and CpCZF2 may be involved in the regulation of stamen development and cause the formation of abnormal flowers in transgenic Arabidopsis plants.

show abstract

Expression of the alfalfa CCCH-type zinc finger protein gene MsZFN delays flowering time in transgenic Arabidopsis thaliana

Cited by 32 publications

References 45 publications

Transcriptome Analysis of Flowering Time Genes under Drought Stress in Maize Leaves

Transcriptome Analysis of Flowering Time Genes under Drought Stress in Maize Leaves

Digital gene expression analysis of male and female bud transition in Metasequoia reveals high activity of MADS-box transcription factors and hormone-mediated sugar pathways

Two C3H Type Zinc Finger Protein Genes, CpCZF1 and CpCZF2, from Chimonanthus praecox Affect Stamen Development in Arabidopsis

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