Expression of the Chimeric Pea PSPAL2 Promoter in Transgenic Tobacco in Response to Fungal Ingress and Injury.

Sriprasertsak, Permpong; Salamah, Andi; Imura, Yoshiyuki; Ichinose, Yuki; Shiraishi, Takeshi; Yamada, Tetsuji

doi:10.3186/jjphytopath.65.123

Cited by 5 publications

(3 citation statements)

References 46 publications

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“…Zhu et al (1995) used a PAL‐GUS reporter construct in rice and tobacco to show increased activity around the wound site in leaves of transgenic plants. A similar situation was observed in wounded leaves of transgenic tobacco transformed with an Arabidopsis (Prasad et al 1995) or pea (Sriprasertsak et al 1999) PAL promoter fused to GUS. Shufflebottom et al (1993) transformed potato, tobacco and Arabidopsis plants with two bean PAL promoters fused with GUS.…”

Section: Resultssupporting

confidence: 64%

“…Changes in PAL activity, together with other enzymes involved in phenylpropanoid biosynthesis, and the accumulation of various phenolic compounds is a plant response to stress (Dixon and Paiva 1995). Wounding is one of many stresses that induces a PAL‐promoter (Bevan et al 1989, Shufflebottom et al 1993, Prasad et al 1995, Zhu et al 1995, Sriprasertsak et al 1999), triggers the accumulation of PAL mRNA (Lawton and Lamb 1987, Ohl et al 1990, Ishizuka et al 1991, Joos and Hahlbrock 1992, Diallinas and Kanellis 1994, Fukusawa‐Akada et al 1996, McConn et al 1997), and increases PAL activity (Minamikawa and Uritani 1965a, Goldstein et al 1972, Wong et al 1974, Tanaka and Uritani 1976, Ke and Saltveit 1989a, Kamo et al 2000, Hisaminato et al 2001). Inhibitors of gene transcription and protein synthesis reduce the wound‐ and ethylene‐induced rise in PAL activity, suggesting that de novo PAL synthesis is required for these responses (Minamikawa and Uritani 1965b, Hyodo and Yang 1971, Chalutz 1973, Matsushita and Uritani 1975, Ke and Saltveit 1989a).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a wound inducible phenylalanine ammonia‐lyase gene (LsPAL1) from Romaine lettuce leaves

Campos

Nonogaki

Suslow

et al. 2004

Physiologia Plantarum

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Phenylalanine ammonia‐lyase (PAL) catalyses the first step controlling the rate of phenylpropanoid metabolism. Wounding is a ubiquitous stress in nature and in the harvesting and preparation of fruits and vegetables that induces an increase in PAL activity, an accumulation of phenolic compounds and subsequent tissue browning. A wound‐inducible PAL gene (LsPAL1) was isolated from Romaine lettuce by RT‐PCR. The putative protein encoded by LsPAL1 is similar to predictive polypeptides sequences for other PALs. The kinetics of PAL mRNA accumulation is similar to those of induced PAL enzyme activity, with enzyme activity following mRNA accumulation by 12 h. Wound‐induced PAL transcripts accumulated in cells close to the wound sites. Tissue printing showed that PAL mRNA was associated with tissue next to the epidermis and vascular bundles. A heterologous PAL protein was expressed in E. coli and was found to show significant PAL activity.

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Section: Resultssupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a wound inducible phenylalanine ammonia‐lyase gene (LsPAL1) from Romaine lettuce leaves

Campos

Nonogaki

Suslow

et al. 2004

Physiologia Plantarum

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“…There fore, it seems prob a ble that the analysed genes are ac ti vated by dif fer ent stim uli and are spe cial ised in ful fill ing dif fer ent tasks. Still, this hy poth e sis of func tional spe ciali sa tion and dif ferent con trol of both pea PA L genes was not confirmed un equiv o cally by any of the re ported re sults of North ern hy bridi sa tion, tran sient ex pres sion in pea proto plasts, or anal y ses of re porter gene expres sion in trans gen ic to bacco , Kawamata et al 1997, Seki et al 1999, Sriprasertsak et al 1999). …”

Section: Introductionmentioning

confidence: 70%

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

2005

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Key words: lu ci fer ase (LUC); phenylalanine ammo nia-lyase (PAL); ul tra vi o let ra di a tion (UV); β-glucuronidase (GUS) Ab stractThe gene fu sion sys tem was used to study UV light-con trol of PS PAL1 and PS PAL2 genes en cod ing phenylalanine am monia-lyase of pea. The in duc tion of pea PAL pro mot ers was analysed in trans gen ic to bacco plants. Bi nary plasmids (de riv atives of pBI101.2 vec tor) con tain ing 5' reg u la tory frag ments of PS PAL1 and PS PAL2 linked to re porter genes (GUS, LUC) were con structed. The anal y ses were per formed with the use of sin gle con structs (con tain ing one vari ant of PS PAL pro moter and one re porter gene) and dual con structs (con tain ing both PS PAL1 and PS PAL2 pro mot ers con nected with dif fer ent reporter genes). The use of dual con structs en abled the eval u ation of both PS PAL pro mot ers ac tiv ity in the same plant. The anal y ses of in vi tro grown plants have shown that both PAL pro mot ers are strongly in duced in leaves sub jected to UV ra dia tion. In some cases, the UV-stim u lated ex pres sion ex ceeded the ex posed ar eas. This phe nom e non was ob served more of ten in the leaves of plants con tain ing the PS PAL1::GUS than PS PAL2::GUS con struct. Re moval of boxes 2, 4, 5 from PS PAL1 pro moter and de le tion of its 5'end re gion (-339 to -1394) decreases the level of gene ex pres sion but does not elim i nate its re spon sive ness to UV.

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Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack

et al. 2000

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Regulatory elements in the promoter of phenylalanine ammonia-lyase gene 1 of pea (PSPALI) in response to nonpathogenic attack were identified by in uivo footprinting analysis. The footprints determined AC-rich sequences, Box-I and Box-11, that were conserved at similar positions in the phenylpropanoid gene promoters from several plants. To reveal the functions of the AC-rich sequence in nonpathogen-responsiveness, we constructed Box-I-deletion PSPALl promoter (&-I) with GUS reporter gene and transformed it into tobacco plant. The dB-I had reduced basal expression and a complete loss of nonpathogen-responsiveness. These results indicate the essentiality of Box-I for PSPALl activation induced by nonpathogenic attack.

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Expression of the Chimeric Pea PSPAL2 Promoter in Transgenic Tobacco in Response to Fungal Ingress and Injury.

Abstract: The 5•OE-upstream

Cited by 5 publications

References 46 publications

Isolation and characterization of a wound inducible phenylalanine ammonia‐lyase gene (LsPAL1) from Romaine lettuce leaves

Isolation and characterization of a wound inducible phenylalanine ammonia‐lyase gene (LsPAL1) from Romaine lettuce leaves

Control of phenylalanine ammonia-lyase gene promoters from pea by UV radiation

Importance of AC-rich Element on Pea Phenylalanine Ammonia-Lyase Gene 1 Promoter for Expression Induced by Nonpathogenic Attack

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