Expression of the Cydia pomonella granulovirus matrix metalloprotease enhances Autographa californica multiple nucleopolyhedrovirus virulence and can partially substitute for viral cathepsin

Ishimwe, Egide; Hodgson, Jeffrey J.; Passarelli, A. Lorena

doi:10.1016/j.virol.2015.02.022

Cited by 24 publications

(12 citation statements)

References 55 publications

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“…The proteins derived from VG008 OBs increased 27% the larvae mortality caused by SfCOL NPV, demonstrating that they are responsible for this enhancing activity. Several studies using recombinants proteins have also demonstrated the enhancer activity of these proteins in different granulovirus coinfection [13,25,35,37].…”

Section: Discussionmentioning

confidence: 99%

Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)

Otálora

Valderrama

Ramos

et al. 2019

Viruses

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The ability of the isolate VG008 of S. frugiperda granulovirus (SpfrGV) to enhance the infectivity of the isolate SfCOL of S. frugiperda multiple nucleopolyhedrovirus (SpfrMNPV) was evaluated on S. frugiperda larvae. Bioassays were performed with mixtures by using different proportions 90%:10% (M1), 95%:5% (M2) and 97.5%:2.5% (M3) of SfCOL:VG008, respectively. All mixtures showed higher insecticidal activity that SfCOL. The mixture M3 showed the highest enhancement of SfCOL reducing 11.40 times the Mean Lethal Concentration and 96 h in the Mean Time to Death. The enhancer activity of proteins derived from VG008 (GVPs) were also evaluated in mixture with SfCOL. The GVPs increased 27% larval mortality caused by SfCOL and damaged the peritrophic membrane of S. litura larvae, suggesting that the key point in this enhancing activity is the initial step of the larva colonization, the midgut infection. M3 was formulated and evaluated under greenhouse conditions in maize plants using different doses. The highest efficacy was obtained with the highest dose of M3 (8 × 1011 OBs/ha), which was similar to that found when formulated SfCOL was applied using an approximately twofold higher dose. The viral mixture M3 was selected as the active ingredient for developing a new biopesticide for a more efficient management of the pest in the field.

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Section: Discussionmentioning

confidence: 99%

Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)

Otálora

Valderrama

Ramos

et al. 2019

Viruses

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“…Whereas the loss of the cathepsin and chitinase genes is a common event among the betabaculoviruses [ 10 ], the matrix metalloproteinase gene is present in all betabaculoviruses sequenced to date [ 20 ]. The expression of a functional CpGV-encoded metalloproteinase into the Autographa californica multiple nucleopolyhedrovirus (AcMNPV) genome enhanced the virus virulence, promoted larval melanization, and could partially substitute for the viral cathepsin [ 21 ].…”

Section: Resultsmentioning

confidence: 99%

A betabaculovirus encoding a gp64 homolog

et al. 2016

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BackgroundA betabaculovirus (DisaGV) was isolated from Diatraea saccharalis (Lepidoptera: Crambidae), one of the most important insect pests of the sugarcane and other monocot cultures in Brazil.ResultsThe complete genome sequence of DisaGV was determined using the 454-pyrosequencing method. The genome was 98,392 bp long, which makes it the smallest lepidopteran-infecting baculovirus sequenced to date. It had a G + C content of 29.7 % encoding 125 putative open reading frames (ORF). All the 37 baculovirus core genes and a set of 19 betabaculovirus-specific genes were found. A group of 13 putative genes was not found in any other baculovirus genome sequenced so far. A phylogenetic analysis indicated that DisaGV is a member of Betabaculovirus genus and that it is a sister group to a cluster formed by ChocGV, ErelGV, PiraGV isolates, ClanGV, CaLGV, CpGV, CrleGV, AdorGV, PhopGV and EpapGV. Surprisingly, we found in the DisaGV genome a G protein-coupled receptor related to lepidopteran and other insect virus genes and a gp64 homolog, which is likely a product of horizontal gene transfer from Group 1 alphabaculoviruses.ConclusionDisaGV represents a distinct lineage of the genus Betabaculovirus. It is closely related to the CpGV-related group and presents the smallest genome in size so far. Remarkably, we found a homolog of gp64, which was reported solely in group 1 alphabaculovirus genomes so far.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-016-2408-9) contains supplementary material, which is available to authorized users.

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“…ODV initiates infection in the midgut cells of the host insects and is responsible for primary infection, while BV infects other tissues of the insect and is responsible for systemic or secondary infections [ 7 ]. Autographa californica multiple nucleopolyhedrovirus (AcMNPV), a model virus of the baculoviridae, has been extensively studied [ 8 , 9 , 10 , 11 , 12 , 13 ]. Further development of these applications requires further insight into the cell entry pathway.…”

Section: Introductionmentioning

confidence: 99%

Autographa Californica Multiple Nucleopolyhedrovirus Enters Host Cells via Clathrin-Mediated Endocytosis and Direct Fusion with the Plasma Membrane

Qin

Lei

et al. 2018

Viruses

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The cell entry mechanism of Autographa californica multiple nucleopolyhedrovirus (AcMNPV) is not fully understood. Previous studies showed that AcMNPV entered host cells primarily through clathrin-mediated endocytosis, and could efficiently infect cells via fusion with the plasma membrane after a low-pH trigger. However, whether AcMNPV enters cells via these two pathways simultaneously, and the exact manner in which AcMNPV particles are internalized into cells remains unclear. In this study, using single-virus tracking, we observed that AcMNPV particles were first captured by pre-existing clathrin-coated pits (CCP), and were then delivered to early endosomes. Population-based analysis of single-virus tracking and quantitative electron microscopy demonstrated that the majority of particles were captured by CCPs and internalized via invagination. In contrast, a minority of virus particles were not delivered to CCPs, and were internalized through direct fusion with the plasma membrane without invagination. Quantitative electron microscopy also showed that, while inhibition of CCP assembly significantly impaired viral internalization, inhibition of endosomal acidification blocked virus particles out of vesicles. Collectively, these findings demonstrated that approximately 90% of AcMNPV particles entered cells through clathrin-mediated endocytosis and 10% entered via direct fusion with the plasma membrane. This study will lead toward a better understanding of AcMNPV infection.

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Expression of the Cydia pomonella granulovirus matrix metalloprotease enhances Autographa californica multiple nucleopolyhedrovirus virulence and can partially substitute for viral cathepsin

Cited by 24 publications

References 55 publications

Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)

Bio-Insecticidal Potential of Nucleopolyhedrovirus and Granulovirus Mixtures to Control the Fall Armyworm Spodoptera frugiperda (J.E. Smith, 1797) (Lepidoptera: Noctuidae)

A betabaculovirus encoding a gp64 homolog

Autographa Californica Multiple Nucleopolyhedrovirus Enters Host Cells via Clathrin-Mediated Endocytosis and Direct Fusion with the Plasma Membrane

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