Expression of the Genes for the α- and β-Subunits of Pyrophosphate-Dependent Phosphofructokinase in Germinating and Developing Seeds from <i>Ricinus communis</i>

Blakeley, Stephen D.; Crews, Linda; Todd, James F.; Dennis, David T.

doi:10.1104/pp.99.3.1245

Cited by 16 publications

(12 citation statements)

References 26 publications

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“…The pattern of expression of the PGM gene, and the accumulation of PGM polypeptides and enzyme activity in developing and germinating castor endosperm are in a good agreement with similar studies for other cytosolic glycolytic enzymes in this tissue [1,33]. It is believed that the level of glycolytic enzyme activity parallels the period of fatty acid biosynthesis in developing castor endosperm, and the rate of utilization of stored fatty acids in the endosperm during seed germination.…”

Section: Discussionsupporting

confidence: 71%

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Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Huang

Blakeley

McALEESE³

et al. 1993

Plant Mol Biol

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Cofactor-independent phosphoglyceromutase (PGM) was purified to homogeneity from developing castor seed endosperm. Immunological characterization using monospecific antisera raised against this protein indicates that the enzyme is located in the cytosol and that there is no immunologically related polypeptide in the leucoplast from this tissue. Isolation and sequence determination of full-length cDNA clones for castor and tobacco PGM demonstrate that the protein is highly conserved in these plants and is closely related to the maize enzyme. A comparison of the amino acid sequence of peptides derived from Neurospora crassa PGM with the cofactor-independent enzyme from higher plants demonstrated that they are related and may have diverged from a common ancestral gene. The previously proposed relationship between higher-plant PGM and alkaline phosphatases is not supported by sequence analysis of the castor and tobacco enzymes. Expression of the single castor cytosolic PGM gene correlates well with other cytosolic glycolytic genes in developing and germinating castor seeds, and with the appearance of enzyme activity and PGM polypeptides in these tissues.

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Section: Discussionsupporting

confidence: 71%

“…Southern hybridization [40] was carried out according to the manufacturer's instructions. Northern blot analysis was done exactly as described previously [1 ]. Total RNA (15 #~g) isolated from different stages of developing and germinating castor endosperm was loaded in each lane of the gel.…”

Section: Southern and Northern Blot Analysismentioning

confidence: 99%

Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Huang

Blakeley

McALEESE³

et al. 1993

Plant Mol Biol

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“…The subgroup of the small subunits (monomers of »320 amino acids) includes the subunits of Amycolatopsis methanolica (Alves et al 1996), Trichomonas vaginalis (Mertens et al 1998) and Thermoproteus tenax (Siebers et al 1998), which are more closely related to the eubacterial ATPPFKs. The subgroup of the large subunits (monomers of »550 amino acids) includes the plant PPi-PFK subunits (Carlisle et al 1990;Blakeley et al 1992), some amitochondriate protists, such as Amycolatopsis methanolica (Alves et al 1996) and Entamoeba histolytica (Mertens et al 1998;Deng et al 1998), and two bacterial groups, the spirochetes Borrelia burgdorferi (Deng et al 1999) and Treponema pallidum (Roberson et al 2000), and Chlamydia (Stephens et al 1998). The large PPi-PFKs in plants are heterotetramers ( ) 2 , and the -subunit of the abovecited bacteria and protists appears to be derived from plants by horizontal transfer (Stephens et al 1998).…”

Section: Transposable Elements-mediated Transduction Of Host Gene Framentioning

confidence: 98%

Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants

et al. 2008

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Transposable elements are major components of plant genomes and they influence their evolution, acting as recombination hot spots, acquiring specific cell functions or becoming part of protein-coding regions. The latter is the subject of the present analysis. This study is a report on the annotation of transposable elements (TEs) in expressed sequences of Coffea arabica, Coffea canephora and Coffea racemosa, showing the occurrence of 383 ESTs and 142 unigenes with TE fragments in these three Coffea species. Based on selected unigenes, it was possible to suggest 26 putative proteins with TE-cassette insertions, demonstrating a likely contribution to protein variability. The genes for two of those proteins, the fertility restorer (FR) and the pyrophosphate-dependent phosphofructokinase (PPi-PFKs) genes, were selected for evaluating the impact of TE-cassettes on host gene evolution of other plant genomes (Arabidopsis thaliana, Oryza sativa and Populus trichocarpa). This survey allowed identifying a FR gene in O. sativa harboring multiple insertions of LTR retrotransposons that originated new exons, which however does not necessarily mean a case of molecular domestication. A possible transduction event of a fragment of the PPi-PFK beta-subunit gene mediated by Helitron ATREPX1 in Arabidopsis thaliana was also highlighted.

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“…The mRNA expression is not directly related to subunit composition in specific tissues, indicating that there is transcriptional and tissue-specific regulation of expression of the PFP subunits (Blakeley et al 1992;Suzuki et al 2003). …”

Section: Ejpfpa-fructosementioning

confidence: 96%

Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

Qin

Kaas

Wu³

et al. 2014

Tree Genetics & Genomes

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Pyrophosphate-dependent phosphofructokinase (PFP) catalyses the reversible phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate in the glycolysis pathway. Two full-length complementary DNAs encoding putative PFP α-and β-subunits, named EjPFPa and EjPFPb1, were isolated by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of the cDNA ends (RACE) from Eriobotrya japonica Lindl. (loquat). The other β-subunit gene was identified from transcriptome data obtained by Illumina sequencing, designated as EjPFPb2. They share up to 88 % identity with other plant PFP α/β-subunits. EjPFPa and EjPFPb clustered separately in clades of plant PFP α-or β-subunits, respectively. EjPFPa and EjPFPb were both shown to be mainly localised in the cell membrane using confocal microscopy of GFP fusion proteins 35S:EjPFPa/b-GFP. Monitoring the dynamic changes of transcripts and proteins demonstrated that EjPFPa and EjPFPb did not show coordinated expression during fruit development in loquat. The transcript levels of EjPFPa and EjPFPb in the leaves of loquat seedlings were significantly enhanced after 3 h of treatment with 0.5, 1.0 and 1.5 M fructose or glucose, which indicates that EjPFPa and EjPFPb are modulated by fructose and glucose in vivo. Transgenic tobacco plants overexpressed EjPFPa did not show visible phenotype changes, while plants overexpressing EjPFPb1 grew faster at the cost of reduced leaf size and sucrose content. Meanwhile, fructose content increased in growing plants compared with wild-type plants.The results confirm that genes of the two subunits from loquat PFP have different transcriptional regulation systems and play different roles in carbohydrate metabolism. Elevation of the PFP β-subunit gene expression can partly impact the glycolytic carbon allocation in growing plants.

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Expression of the Genes for the α- and β-Subunits of Pyrophosphate-Dependent Phosphofructokinase in Germinating and Developing Seeds from Ricinus communis

Cited by 16 publications

References 26 publications

Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Higher-plant cofactor-independent phosphoglyceromutase: purification, molecular characterization and expression

Transposable elements in Coffea (Gentianales: Rubiacea) transcripts and their role in the origin of protein diversity in flowering plants

Characterisation of the subunit genes of pyrophosphate-dependent phosphofructokinase from loquat (Eriobotrya japonica Lindl.)

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