Expression of the genes for guanyl-specific ribonucleases from Bacillus intermedius and Bacillus pumilus is regulated by the two component signal transduction system PhoP-PhoR in B. subtilis

Abstract

Promoters of the genes for guanyl-specific ribonucleases, secreted by B. intermedius (binase) and B. pumilus (Rnase Bp) in phosphate deficient conditions, contain regions similar to appropriate consensus sequences in promoters of the PHO regulated genes of B. subtilis. A number of genes expressed in response to phosphate starvation in B. subtilis are regulated by the two component signal transduction system PhoP-PhoR. Expression of recombinant genes for binase and RNase Bp in B. subtilis strains with mutations in the regulatory protein genes of the PHO regulon was studied. Their expression is strongly regulated by the regulatory proteins of the B. subtilis PHO regulon. z

A Phosphate Starvation-Inducible Ribonuclease of Bacillus licheniformis

“…Prevoius studies described that binase-like and barnase- like RNases were expressed differently to phosphate concentrations in the medium [22,27,28]. Whereas barnaselike RNases respond less sensitively to the concentration of phosphate in the medium, binase-like RNases are significantly induced at low phosphate concentrations.…”

A novel secreted ribonuclease from Bacillus intermedius: gene structure and regulatory control

“…further upstream, positions (Znamenskaya et al 1999). It has been demonstrated by two-dimensional electrophoresis that not all the proteins that are induced by phosphate starvation are controlled by the PhoP-PhoR two-component system.…”

“…The proteins are closely related, consisting of 109 and 110 amino acids, respectively, and show 81% sequence identity. In spite of this similarity, the biosynthesis of binase I is induced in response to phosphate starvation (due to the presence of an upstream dual pho-box sequence, which is assumed to interact with the phosphorylated regulator protein PhoP in B. subtilis), while synthesis of barnase is independent of the concentration of extracellular inorganic phosphate (Znamenskaya et al 1999).…”

Three‐step procedure for preparation of pure Bacillus altitudinis ribonuclease

“…1). It was shown earlier that inorganic phosphates inhibit the production of guanyl-preferring RNases [26] and [27]. On the basis of this data, we performed experiments using a phosphate deficient medium for B. altitudinis growth.…”