Expression of the influenza M2 protein in three different eukaryotic cell lines

Esghaei, Maryam; Monavari, Seyed Hamid Reza; Tavassoti-Kheiri, M; Shamsi-Shahrabadi, M; Heydarchi, Behnaz; Farahmand, Behrokh; Sabbaghan, Maryam; Fotouhi, Fatemeh

doi:10.1016/j.jviromet.2011.10.016

Cited by 10 publications

(14 citation statements)

References 31 publications

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“…Generally, influenza A viruses infect both humans and such animals as ducks, chickens, pigs, whales, horses, seals and cats; while, influenza B viruses circulate primarily in humans . Based on hemagglutinin (HA) amino acid differences, influenza A is divided into 16 different HA subtypes (H1‐H16), and also nine different NA subtypes (N1‐N9) . The main subtypes of influenza based on pathogenesis and epidemiology aspects include H1N1, H5N1, H3N2, H7N7 and H9N2 .…”

Section: Introduction and Objectivesmentioning

confidence: 99%

“…5 Based on hemagglutinin (HA) amino acid differences, influenza A is divided into 16 different HA subtypes (H1-H16), and also nine different NA subtypes (N1-N9). 6,7 The main subtypes of influenza based on pathogenesis and epidemiology aspects include H1N1, H5N1, H3N2, H7N7 and H9N2. 8 The most symptoms of influenza infection can be mild to severe include fever, sore throat, nasal discharge, myalgia, headache and cough.…”

Section: Introduction and Obj Ec Ti Vesmentioning

confidence: 99%

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Prevalence of influenza A infection in the Middle‐East: A systematic review and meta‐analysis

Moghoofei

Monavari

Mostafaei

et al. 2018

Clinical Respiratory J

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Section: Introduction and Objectivesmentioning

confidence: 99%

Section: Introduction and Obj Ec Ti Vesmentioning

confidence: 99%

Prevalence of influenza A infection in the Middle‐East: A systematic review and meta‐analysis

Moghoofei

Monavari

Mostafaei

et al. 2018

Clinical Respiratory J

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“…NS1 binds to p85β, the regulatory subunit of PI3K, while M2 keeps autophagy at moderate levels by limiting the degree of lysosome fusion with autophagosomes . M2 protein, a virus‐specific membrane protein, is expressed at the surface of infected cells. It has a short (24 residues long), highly conserved extracellular N‐terminal domain, an internal hydrophobic anchorage domain (approximately 19 residues long), and a C‐terminal amphipathic cytoplasmic domain (54 residues long) facing inside the viral particle .…”

Section: Viruses and Autophagymentioning

confidence: 99%

Autophagy: The multi‐purpose bridge in viral infections and host cells

Abdoli

Alirezaei

Mehrbod

et al. 2018

Reviews in Medical Virology

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Autophagy signaling pathway is involved in cellular homeostasis, developmental processes, cellular stress responses, and immune pathways. The aim of this review is to summarize the relationship between autophagy and viruses. It is not possible to be fully comprehensive, or to provide a complete "overview of all viruses". In this review, we will focus on the interaction of autophagy and viruses and survey how human viruses exploit multiple steps in the autophagy pathway to help viral propagation and escape immune response. We discuss the role that macroautophagy plays in cells infected with hepatitis C virus, hepatitis B virus, rotavirus gastroenteritis, immune cells infected with human immunodeficiency virus, and viral respiratory tract infections both influenza virus and coronavirus.

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“…Full length of the M2 gene has previously been amplified by PCR using influenza Virus mRNA, cloned into pGEMTeasy vector and sequenced (15). A specific primer set previously used for amplification of M2 DNA was modified to incorporate desired restriction enzyme sites (BamHI and Hind III) in the 5' ends of the gene.…”

Section: M2 Gene Amplification and Subcloning Into Pet28amentioning

confidence: 99%

“…The results showed that the recombinant M2 protein was about 21% of the total BL21 proteins. We previously constructed recombinant pQE30-M2 plasmid and expressed the M2 protein in E.coli host strain M15 (15). M2 protein production in BL21 and M15 cells was compared using Quantity One software (Fig.…”

Section: Insilico Analysismentioning

confidence: 99%

Over Expression of Influenza Virus M2 Protein in Prokaryotic System

et al. 2012

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Background and Aims: Influenza A virus of Orthomyxoviridae family is able to create pandemic influenza. Vaccination is the most effective way to prevent influenza virus infection. Matrix protein 2 (M2) is a homotetramer ion channel with 97 amino acids length and highly conserved among influenza viruses and is considered for development of a universal influenza vaccine. Materials and Methods:We present here cloning and expression of influenza A virus M2 protein as a fusion with 6-His tag in Escherichia coli BL21 strain. The gene was amplified by PCR and ligated into the prokaryotic expression vector pET28a. The expression of M2 protein was induced by IPTG and confirmed by SDS-PAGE and western blotting. The desired protein was purified with affinity chromatography on a Ni-TED resin column and has to be evaluated in animal models for further studies. Results:The results of sequencing showed that M2 gene was cloned in pET28a properly in frame to histidine tag and the product was confirmed by xpreimmune reaction of monoclonal anti-M2 antibody to recombinant M2 in western blotting. Conclusion: This study might provide a basis for production of a universal and broadspectrum human influenza vaccine.

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Expression of the influenza M2 protein in three different eukaryotic cell lines

Cited by 10 publications

References 31 publications

Prevalence of influenza A infection in the Middle‐East: A systematic review and meta‐analysis

Prevalence of influenza A infection in the Middle‐East: A systematic review and meta‐analysis

Autophagy: The multi‐purpose bridge in viral infections and host cells

Over Expression of Influenza Virus M2 Protein in Prokaryotic System

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