Expression of the Long Intergenic Non-Coding RNA (lincRNA) of the NED25 Gene Modulates the microRNA-125b, STAT3, Nitric Oxide, and Procalcitonin Signaling Pathways in Patients with Sepsis

Le, Yuanjie; Chen, Tongen; Xun, Kai; Ding, Tao

doi:10.12659/msm.907496

Cited by 12 publications

(15 citation statements)

References 33 publications

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“…In healthy individuals all these component peptides, including PCT, are present at very low serum concentrations, and are produced by the neuroendocrine cells in the lungs and thyroid gland (26). Recent papers have studied the possible relationship between calcitonin or PCT and miRNAs (27,28,29,30). miRNAs are implicated in biological processes such as cellular differentiation, proliferation, and apoptosis; they are stable and can be detected in clinical samples and used as biomarkers for the early diagnosis of several diseases (31).…”

Section: Types Of Calcitonin Pro-calcitonin and Molecular Aspects MImentioning

confidence: 99%

ENDOCRINE TUMOURS: Calcitonin in thyroid and extra-thyroid neuroendocrine neoplasms: the two-faced Janus

Giannetta

Guarnotta

Altieri

et al. 2020

European Journal of Endocrinology

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An increased calcitonin serum level is suggestive of a medullary thyroid cancer (MTC), but is not pathognomonic. The possibility of false positives or other calcitonin-secreting neuroendocrine neoplasms (NENs) should be considered. Serum calcitonin levels are generally assessed by immunoradiometric and chemiluminescent assays with high sensitivity and specificity; however, slightly-moderately elevated levels could be attributable to various confounding factors. Calcitonin values >100 pg/mL are strongly suspicious of malignancy, whereas in patients with moderately elevated values (10-100 pg/mL) a stimulation test may be applied to improve diagnostic accuracy. Although the standard protocol and the best gender-specific cut-offs for calcium stimulated calcitonin are still controversial, the fold of the calcitonin increase after stimulation seems to be more reliable. Patients with MTC show stimulated calcitonin values at least 3-4 times higher than the basal values, whereas calcitonin-secreting NENs can be distinguished from a C-cell disease by the absence of or <2-fold response to stimulation. The measurement of calcitonin in fine-needle aspirate washout (FNA-CT) and calcitonin immunocytochemical staining from thyroid nodules are ancillary methods that may significantly improve MTC diagnosis. The present review examines the gray areas in the interpretation of calcitonin measurement in order to provide a tool to clarify the origin of calcitonin secretion and differentiate the behavior of the two-faced Janus of neuroendocrinology: intra-thyroid (MTC) and extra-thyroid NENs.

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Section: Types Of Calcitonin Pro-calcitonin and Molecular Aspects MImentioning

confidence: 99%

ENDOCRINE TUMOURS: Calcitonin in thyroid and extra-thyroid neuroendocrine neoplasms: the two-faced Janus

Giannetta

Guarnotta

Altieri

et al. 2020

European Journal of Endocrinology

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“…Meanwhile, by performing an ROC analysis, we found that only miR-125b and PCT were involved in post-operative infection. Previously, researches have exhibited that the treatment with LPS for the PBMs enhanced the PCT level and decreased the miR-125b level [19]. In addition, the treatment with LPS enhanced the expression of total STAT3 and phosphorylated STAT3, which in turn increased the expression level of PCT [19].…”

Section: Discussionmentioning

confidence: 97%

“…Previously, researches have exhibited that the treatment with LPS for the PBMs enhanced the PCT level and decreased the miR-125b level [19]. In addition, the treatment with LPS enhanced the expression of total STAT3 and phosphorylated STAT3, which in turn increased the expression level of PCT [19]. Nevertheless, previously, it has been indicated that miR-125b and the lincRNA of the gene of NED25 increased the levels of total STAT3 and phosphorylated STAT3 while lowering the expression level of PCT [20].…”

Section: Discussionmentioning

confidence: 99%

Dysregulation of microRNA-125b is involved in the pathogenesis of post-operative infection via modulating the STAT3/procalcitonin (PCT) signaling pathway

2021

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IntroductionProcalcitonin (PCT) has been reported to function as a predictive biomarker of post-operative infection. In this study, we aimed to explore the mechanisms underlying the regulation of PCT expression.Material and methods72 children diagnosed with post-operative infection and 58 children without post-operative infection were recruited in this study. Computational analysis, luciferase assay, real-time PCR, Western-blot analysis, and assays of post-operative C-reactive protein (CRP), erythrocyte sedimentation rate (ESR) and PCT were performed to investigate the mechanisms underlying the regulation of PCT expression.ResultsMiR-125b was found to repress STAT3 expression with putative binding sites in 3’UTR of STAT3. The levels of PCT and miR-125b in non-infection group remained stable from day 0 to day 5, while the level of PCT was increased in the infection group along with a decreased level of miR-125b from day 1 to day 5. The post-operative levels of CRP and ESR in both non-infection and infection groups were evidently increased in a time-dependent manner, but the levels of miR-106b and miR-20a in both non-infection and infection groups remained stable. The area under the curve (AUC) values of PCT, CRP, ESR, miR-125b, miR-106b and miR-20a demonstrated that only miR-125b and PCT were involved in infection. Transfection with miR-125b reduced STAT3 expression, while the activation of STAT3 by lipopolysaccharide (LPS) treatment up-regulated PCT production. Finally, miR-125b down-regulated the expression of PCT by targeting STAT3.ConclusionsTaken together, we suggested that miR-125b was involved in the prognosis and diagnosis of poster-operative infection by modulating the signaling pathway of miR-125b/STAT3/PCT.

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“…What is clear is that they influence the transcription, and ultimately translation, of many genes in the aetiologies of different cancers 86,87 and other diseases. 88 As can be seen from Table 1 and Figure 2, depending on the base present, SNP rs694539 changes the sequences of 4 transcripts, and rs2256292 and rs1941404 also alter multiple transcripts. Of the 8 transcripts emanating from the 2 forms of NNMT , only 1 transcript ( NNMT-201 ) goes on to be translated to any significant extent.…”

Section: Nnmt – Association With Homocysteine Metabolism and Illnessmentioning

confidence: 98%

Nicotinamide N-Methyltransferase: Genomic Connection to Disease

Ramsden

Waring

Parsons

et al. 2020

Int J�Tryptophan�Res

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Single-nucleotide polymorphisms (SNPs) in and around the nicotinamide N-methyltransferase (NNMT) gene are associated with a range of cancers and other diseases and conditions. The data on these associations have been assembled, and their strength discussed. There is no evidence that the presence of either the major or minor base in any SNP affects the expression of nicotinamide N-methyltransferase. Nevertheless, suggestions have been put forward that some of these SNPs do affect NNMT expression and thus homocysteine metabolism. An alternative idea involving non-coding messenger RNAs (mRNAs) is suggested as a possible mechanism whereby health is influenced. It is postulated that these long, non-coding NNMT mRNAs may exert deleterious effects by interfering with the expression of other genes. Neither hypothesis, however, has experimental proof, and further work is necessary to elucidate NNMT genetic interactions.

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Expression of the Long Intergenic Non-Coding RNA (lincRNA) of the NED25 Gene Modulates the microRNA-125b, STAT3, Nitric Oxide, and Procalcitonin Signaling Pathways in Patients with Sepsis

Cited by 12 publications

References 33 publications

ENDOCRINE TUMOURS: Calcitonin in thyroid and extra-thyroid neuroendocrine neoplasms: the two-faced Janus

ENDOCRINE TUMOURS: Calcitonin in thyroid and extra-thyroid neuroendocrine neoplasms: the two-faced Janus

Dysregulation of microRNA-125b is involved in the pathogenesis of post-operative infection via modulating the STAT3/procalcitonin (PCT) signaling pathway

Nicotinamide N-Methyltransferase: Genomic Connection to Disease

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