Expression of the long noncoding RNA RP11‐169D4.1‐001 in Hypopharyngeal Squamous cell carcinoma tissue and its clinical significance

Osteosarcoma (OS) is one of the most commonly seen malignant bone tumors, which has higher incidence in both children and teenagers with lung metastasis. 1,2 Three main strategies, namely surgical resection, chemotherapy, and radiotherapy are applied for patients currently. 3 Neoadjuvant chemotherapy combined with surgical treatment has been reported to improve the 5-year survival rate to nearly 70% in localized OS over the past three decades, however, chemoresistance to these drugs is still the main obstacle for curing this malignancy, 1,4 thereby leading to poor prognosis, high recurrence, and metastasis. Therefore, identification of effective biomarkers for pathogenesis, prognosis, and metastasis is essential for OS patients. 5 A variety of potential biomarkers have been identified in recent years, which could widely regulate the oncogenesis, prognosis, and metastasis of OS. Nevertheless, the detailed molecular regulatory mechanisms of OS needs further exploration. 6,7 In the last few decades, ground-breaking progress in transcriptional regulators of mammalian genome, known as "non-coding RNAs" (ncRNAs), has become a major concern of molecular biological research worldwide. Although initially considered to be transcriptional noise or so-called "Junk" for lack of coding potential, numerous ncRNAs have been found to play pivotal roles in cellular processes of different diseases, including cancer. [8][9][10] Regulatory ncRNAs are mainly categorized into two classes based on their

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confidence: 99%

Long noncoding RNAs in osteosarcoma via various signaling pathways

Han

Shen

2020

“…In the past, the ncRNA expression spectrum of LSCC mainly focused on miRNA and lncRNA, our previous research revealed that miR‐34a, 10 AC026166.2‐001 11 and RP11‐169D4.1‐001 12 were closely related to the occurrence of LSCC. A recent study found that lncRNA MALAT1 was overexpressed in LSCC tissues and highly correlated with the 5‐year survival of patients; it triggers the resistance of LSCC to chemotherapy drugs by promoting metastasis and inhibiting tumor cell apoptosis 13 .…”

Section: Discussionmentioning

confidence: 99%

tRNA^Ini_CAT inhibits proliferation and promotes apoptosis of laryngeal squamous cell carcinoma cells

Shen

Lin

et al. 2021

Self Cite

Background Laryngeal squamous cell carcinoma (LSCC) brings a heavy blow to the patient's voice. Transfer RNA (tRNA) is a common RNA, the roles of tRNAs in LSCC are largely unknown. Methods The tRNA expression profile in LSCC tissues and adjacent normal tissues was measured by a tRNA qRT‐PCR array. The expression level of tRNAIniCAT in LSCC tissues and plasmas was detected by qRT‐PCR. The receiver operating characteristic (ROC) curve was established. tRNAIniCAT was upregulated by a lentivirus vector in the LSCC cell line. Moreover, tRNAIniCAT was upregulated in LSCC xenograft nude mouse model and the xenografts were used for pathological analysis and transmission electron microscope (TEM) observation. Results The top 10 upregulated tRNAs were tRNALysCTT‐1, tRNALeuTAA, tRNAPheGAA, tRNALeuCAG, tRNATyrATA, tRNAMetCAT, tRNATyrGTA‐1, tRNAThrCGT, tRNATyrGTA‐2, tRNAAlaAGC; and the top 10 downregulated tRNAs were tRNAIniCAT, mt‐tRNAGluTTC, tRNAValCAC‐3, mt‐tRNATrpTCA, mt‐tRNATyrGTA, mt‐tRNALysTTT, mt‐tRNAThrTGT, mt‐tRNAAspGTC, mt‐tRNAAsnGTT, mt‐tRNAProTGG. tRNAIniCAT was downregulated in LSCC tissues and plasma. The area under the ROC curve (AUC) in LSCC tissues and the plasma of patients with LSCC was 0.717 and 0.808, respectively. tRNAIniCAT inhibited LSCC cell proliferation and promoted apoptosis. The in vivo results showed that tRNAIniCAT inhibited the growth of the xenografts and promoted apoptosis. Conclusions This is the first study to provide tRNA expression profiles for LSCC tissues. tRNAIniCAT may be used as a new biomarker for the early diagnosis of LSCC. tRNAIniCAT inhibits cell proliferation and promotes apoptosis in vitro and in vivo.

“…For advanced primary tumor(s) or previously treated dysfunctional larynx, total or partial laryngectomy plays a critical role and remains the primary method of treatment 5 . These surgical methods influence the capacity of phonation and airway protection during swallowing 6 . The need for laryngectomy persists among individuals with a dysfunctional larynx and poor quality of life.…”

Section: Introductionmentioning

confidence: 99%

Tissue engineering of the larynx: A contemporary review

Chen¹,

Shen²,

Shen³

et al. 2020

Self Cite

Objective Tissue engineering has been a topic of extensive research in recent years and has been applied to the regeneration and restoration of many organs including the larynx. Currently, research investigating tissue engineering of the larynx is either ongoing or in the preclinical trial stage. Methods A literature search was performed on the Advanced search field of PubMed using the keywords: “(laryncheal tissue engineering) AND (cartilage regeneration OR scaffolds OR stem cells OR biomolecules).” After applying the selection criteria, 65 articles were included in the study. Results The present review focuses on the rapidly expanding field of tissue‐engineered larynx, which aims to provide stem cell–based scaffolds combined with biological active factors such as growth factors for larynx reconstruction and regeneration. The trend in recent studies is to use new techniques for scaffold construction, such as 3D printing, are developed. All of these strategies have been instrumental in guiding optimization of the tissue‐engineered larynx, leading to a level of clinical induction beyond the in vivo animal experimental phase. Conclusions This review summarizes the current progress and outlines the necessary basic components of regenerative laryngeal medicine in preclinical fields. Finally, it considers the design of scaffolds, support of growth factors, and cell therapies toward potential clinical application.