Expression of the lysophospholipid receptor family and investigation of lysophospholipid-mediated responses in human macrophages

Duong, Chinh Quoc; Bared, Salim Maa; Abu‐Khader, Ahmad; Buechler, Christa; Schmitz, Anna; Schmitz, Gerd

doi:10.1016/j.bbalip.2004.03.002

Cited by 82 publications

(48 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present paper we could show that all S1P receptor subclasses were expressed on BMDM. Characterization of S1P expression patterns of macrophages has shown differing results, most likely due to the heterogeneity of macrophage populations used in different studies [17–21, 23, 34]. While expression of S1P 1 and S1P 2 could be consistently shown in murine and human macrophage populations, expression of the remaining three S1P receptor subclasses was reported more inconsistently.…”

Section: Discussionmentioning

confidence: 99%

“…S1P receptor expression on macrophages has been mainly determined by RT-PCR due to technical difficulties with protein detection. Expression profiles of the known five S1P receptors vary considerably depending on the organ and species origin of the cells analyzed [16–21]. However, S1P signalling affects a myriad of biological functions in various macrophage populations under physiological and disease conditions [10, 22–24].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration

Müller

Bernstorff²,

Heidecke

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

Introduction. Macrophages are key players in complex biological processes. In response to environmental signals, macrophages undergo polarization towards a proinflammatory (M1) or anti-inflammatory (M2) phenotype. Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid that acts via 5 G-protein coupled receptors (S1P1–5) in order to influence a broad spectrum of biological processes. This study assesses S1P receptor expression on macrophages before and after M1 and M2 polarization and performs a comparative analysis of S1P signalling in the two activational states of macrophages. Methods. Bone marrow derived macrophages (BMDM) from C57 BL/6 mice were cultured under either M1- or M2-polarizing conditions. S1P-receptor expression was determined by quantitative RT-PCR. Influence of S1P on macrophage activation, migration, phagocytosis, and cytokine secretion was assessed in vitro. Results. All 5 S1P receptor subclasses were expressed in macrophages. Culture under both M1- and M2-polarizing conditions led to significant downregulation of S1P1. In contrast, M1-polarized macrophages significantly downregulated S1P4. The expression of the remaining three S1P receptors did not change. S1P increased expression of iNOS under M2-polarizing conditions. Furthermore, S1P induced chemotaxis in M1 macrophages and changed cytokine production in M2 macrophages. Phagocytosis was not affected by S1P-signalling. Discussion. The expression of different specific S1P receptor profiles may provide a possibility to selectively influence M1- or M2-polarized macrophages.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration

Müller

Bernstorff²,

Heidecke

et al. 2017

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…Another notable molecule is sphingosine-1-phosphate (S1P), which is a bioactive lipid. Human macrophages express the relevant receptors, S1PR1-4 (17). Among S1PRs, S1PR2 inhibits macrophage migration, and depletion of S1PR2 enhances macrophage recruitment in vivo (18).…”

Section: Recruitment Of Macrophage To Vascular Inflammatory Lesionsmentioning

confidence: 99%

Macrophages in vascular inflammation – From atherosclerosis to vasculitis

et al. 2015

View full text Add to dashboard Cite

The spectrum of vascular inflammatory disease ranges from atherosclerosis and hypertension, widespread conditions affecting large proportions of the population, to the vasculitides, rare syndromes leading to fast and irreversible organ failure. Atherosclerosis progresses over decades, inevitably proceeding through multiple phases of disease and causes its major complications when the vessel wall lesion ruptures, giving rise to lumen-occlusive atherothrombosis. Vasculitides of medium and large arteries progress rapidly, causing tissue ischemia through lumen-occlusive intimal hyperplasia. In both disease entities, macrophages play a decisive role in pathogenesis, but function in the context of other immune cells that direct their differentiation and their functional commitments. In atherosclerosis, macrophages are involved in the removal of lipids and tissue debris and make a critical contribution to tissue damage and wall remodeling. In several of the vasculitides, macrophages contribute to granuloma formation, a microstructural platform optimizing macrophage-T cell interactions, antigen containment and inflammatory amplification. By virtue of their versatility and plasticity, macrophages are able to promote a series of pathogenic functions, ranging from the release of cytokines and enzymes, the production of reactive oxygen species, presentation of antigen and secretion of tissue remodeling factors. However, as short-lived cells that lack memory, macrophages are also amendable to reprogramming, making them promising targets for anti-inflammatory interventions.

show abstract

“…Expression by alveolar macrophages from rat and mouse differed, with S1P 2 and S1P 4 being the only S1PR mRNA expressed by rat, but mouse alveolar macrophages expressed S1P 1-4 , with S1P 2 predominating 202,203 . Human peripheral blood monocytes (PBM) and PBM-derived macrophages produced varying levels of mRNA for S1P 1-5 and LPA 1-5 expression which could be altered dependent upon phenotype, i.e., differentiation to foam cells could increase or decrease expression of certain receptors, and human alveolar macrophages also exhibited a different receptor pattern 81,204,205 . Differences in receptor expression can likely be explained by cell location and isolation methods, as monocytes and macrophages are notoriously susceptible to alterations in activation status and protein expression caused by manipulation 206,207 .…”

Section: Immune Systemmentioning

confidence: 99%

Regulation of Mammalian Physiology, Development, and Disease by the Sphingosine 1-Phosphate and Lysophosphatidic Acid Receptors

2011

View full text Add to dashboard Cite

Expression of the lysophospholipid receptor family and investigation of lysophospholipid-mediated responses in human macrophages

Cited by 82 publications

References 36 publications

Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration

Differential S1P Receptor Profiles on M1- and M2-Polarized Macrophages Affect Macrophage Cytokine Production and Migration

Macrophages in vascular inflammation – From atherosclerosis to vasculitis

Regulation of Mammalian Physiology, Development, and Disease by the Sphingosine 1-Phosphate and Lysophosphatidic Acid Receptors

Contact Info

Product

Resources

About