Expression of the Medicago truncatula MtDof32 transcription factor regulates plant growth and enhances abiotic stress tolerances in transgenic Arabidopsis

Guo, Tao; Wang, Shumin; Xu, Lixin; Li, Yinruizhi; Chao, Yuehui; Han, Liebao

doi:10.1016/j.envexpbot.2020.104339

Cited by 10 publications

(6 citation statements)

References 58 publications

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“…Dof-type TFs, which are characterized by a DNA-binding zinc finger, have emerged as critical regulators of plant environmental stress responses. Examples include MtDof32 in A. thaliana , which confers enhanced tolerance to osmotic and salt stress [ 64 ], and GhDof1 in cotton, contributing to salinity and cold tolerance [ 65 ]. The overexpression of the cotton GhDof1.7 gene in A. thaliana transgenic plants exhibited increased salinity tolerance, accompanied by reduced ROS accumulation and elevated activities of superoxide dismutase (SOD) and catalase (CAT) enzymes [ 66 ].…”

Section: Discussionmentioning

confidence: 99%

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

da Silva,

Silva,

Ferreira-Neto

et al. 2024

Plants

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Helicases, motor proteins present in both prokaryotes and eukaryotes, play a direct role in various steps of RNA metabolism. Specifically, SF2 RNA helicases, a subset of the DEAD-box family, are essential players in plant developmental processes and responses to biotic and abiotic stresses. Despite this, information on this family in the physic nut (Jatropha curcas L.) remains limited, spanning from structural patterns to stress responses. We identified 79 genes encoding DEAD-box RNA helicases (JcDHX) in the J. curcas genome. These genes were further categorized into three subfamilies: DEAD (42 genes), DEAH (30 genes), and DExH/D (seven genes). Characterization of the encoded proteins revealed a remarkable diversity, with observed patterns in domains, motifs, and exon–intron structures suggesting that the DEAH and DExH/D subfamilies in J. curcas likely contribute to the overall versatility of the family. Three-dimensional modeling of the candidates showed characteristic hallmarks, highlighting the expected functional performance of these enzymes. The promoter regions of the JcDHX genes revealed potential cis-elements such as Dof-type, BBR-BPC, and AP2-ERF, indicating their potential involvement in the response to abiotic stresses. Analysis of RNA-Seq data from the roots of physic nut accessions exposed to 150 mM of NaCl for 3 h showed most of the JcDHX candidates repressed. The protein–protein interaction network indicated that JcDHX proteins occupy central positions, connecting events associated with RNA metabolism. Quantitative PCR analysis validated the expression of nine DEAD-box RNA helicase transcripts, showing significant associations with key components of the stress response, including RNA turnover, ribosome biogenesis, DNA repair, clathrin-mediated vesicular transport, phosphatidyl 3,5-inositol synthesis, and mitochondrial translation. Furthermore, the induced expression of one transcript (JcDHX44) was confirmed, suggesting that it is a potential candidate for future functional analyses to better understand its role in salinity stress tolerance. This study represents the first global report on the DEAD-box family of RNA helicases in physic nuts and displays structural characteristics compatible with their functions, likely serving as a critical component of the plant’s response pathways.

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Section: Discussionmentioning

confidence: 99%

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

da Silva,

Silva,

Ferreira-Neto

et al. 2024

Plants

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“…Thus, MtRAV3 and MtSPL8 might play different roles in morphological development. However, Guo et al (2021) find that overexpression of MtDof32 (DNA-binding one zinc finger 32) in Arabidopsis results in reduced branches and enlarged leaves, but still have enhanced osmotic and salt tolerance. Although both MtDof32 and MtRAV3 enhance drought and salt tolerance in transgenic plants, they regulate different morphological development.…”

Section: Drought Stressmentioning

confidence: 99%

Tolerant mechanism of model legume plant Medicago truncatula to drought, salt, and cold stresses

et al. 2022

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Legume plants produce one-third of the total yield of primary crops and are important food sources for both humans and animals worldwide. Frequent exposure to abiotic stresses, such as drought, salt, and cold, greatly limits the production of legume crops. Several morphological, physiological, and molecular studies have been conducted to characterize the response and adaptation mechanism to abiotic stresses. The tolerant mechanisms of the model legume plant Medicago truncatula to abiotic stresses have been extensively studied. Although many potential genes and integrated networks underlying the M. truncatula in responding to abiotic stresses have been identified and described, a comprehensive summary of the tolerant mechanism is lacking. In this review, we provide a comprehensive summary of the adaptive mechanism by which M. truncatula responds to drought, salt, and cold stress. We also discuss future research that need to be explored to improve the abiotic tolerance of legume plants.

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“…Interestingly, walnut JrDof3 activates JrGRAS2, thereby reinforcing the regulation of HSP and heat stress response ( Yang et al, 2018 ). In addition, Medicago MtDof32 plays a positive role in regulating the expression of stress-related genes (i.e., upregulation of DROUGHT-INDUCED 21, AtDI21 ; SENESCENCE-ASSOCIATED GENE 21 ; AtCOR15A ) leading to enhanced osmotic and salt tolerances in Arabidopsis ( Guo et al, 2021 ). These examples point to the role of Dof in the regulation of stress-related genes and potentially enhanced abiotic stress tolerance especially heat ( Figure 1C ).…”

Section: Dof Associated With Abiotic Stressmentioning

confidence: 99%

Emerging Roles of Plant DNA-Binding With One Finger Transcription Factors in Various Hormone and Stress Signaling Pathways

et al. 2022

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Coordinated transcriptional regulation of stress-responsive genes orchestrated by a complex network of transcription factors (TFs) and the reprogramming of metabolism ensure a plant’s continued growth and survival under adverse environmental conditions (e.g., abiotic stress). DNA-binding with one finger (Dof) proteins, a group of plant-specific TF, were identified as one of several key components of the transcriptional regulatory network involved in abiotic stress responses. In many plant species, Dofs are often activated in response to a wide range of adverse environmental conditions. Dofs play central roles in stress tolerance by regulating the expression of stress-responsive genes via the DOFCORE element or by interacting with other regulatory proteins. Moreover, Dofs act as a key regulatory hub of several phytohormone pathways, integrating abscisic acid, jasmonate, SA and redox signaling in response to many abiotic stresses. Taken together, we highlight a unique role of Dofs in hormone and stress signaling that integrates plant response to adverse environmental conditions with different aspects of plant growth and development.

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Expression of the Medicago truncatula MtDof32 transcription factor regulates plant growth and enhances abiotic stress tolerances in transgenic Arabidopsis

Cited by 10 publications

References 58 publications

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

DEAD-Box RNA Helicase Family in Physic Nut (Jatropha curcas L.): Structural Characterization and Response to Salinity

Tolerant mechanism of model legume plant Medicago truncatula to drought, salt, and cold stresses

Emerging Roles of Plant DNA-Binding With One Finger Transcription Factors in Various Hormone and Stress Signaling Pathways

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