Expression of the oxytocin gene in the large cells of the bovine corpus luteum

Fehr, Susanne; Ivell, Richard; Koll, R.; Schams, D.; Fields, Michael J.; Richter, Dietmar

doi:10.1016/0014-5793(87)81295-4

Cited by 66 publications

(29 citation statements)

References 15 publications

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“…Hybridization was performed in the presence of 4 ng of 3sS-labelled and/or 40 ng of'biotinylated cRNA probes as described [14], except that the final dehydration steps through ascending ethanol concentrations were omitted.…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons

et al. 1988

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The genes for the hypothalamic hormones vasopressin and oxytocin are located in close proximity to each other within the rat genome. They are separated by only approx. 11 kbp of DNA sequence and oriented in such a way that their transcription occurs on opposite DNA strands. Although the two genes are structurally very similar including common potential regulatory elements in their putative promoter regions, they are expressed in discrete populations of magnocellular neurons of the hypothalamus. In rats placed under osmotic stress, the vasopressin gene is upregulated; concomitantly transcription of the oxytocin gene is also stimulated. To address the question of whether this coordinated rise in oxytocin-encoding mRNA is the result of switching on oxytocin gene transcription in vasopressinergic neurons, in situ hybridization with double labelled cRNA probes was carried out. Biotinylated and [a-~sS]CTP labelled antisense cRNA probes specific for either vasopressin or oxytocin mRNA were constructed and hybridized to hypothalamic sections from saltloaded rats. The results demonstrate that upregulation of oxytocin gene transcription is restricted solely to oxytocinergic cells; no oxytocin gene transcripts can be detected in vasopressinergic neurons.

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Section: In Situ Hybridizationmentioning

confidence: 99%

Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons

et al. 1988

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“…A P 4 desempenha funções primordiais no sistema reprodutor das fêmeas, como a regulação e manutenção do ciclo estral e o miorrelaxamento uterino, além de estimular a proliferação de células no endométrio, fundamentais para propiciar as condições necessárias para o desenvolvimento do embrião e do feto (Banks 1991, Reynolds et al 1994, Rafez & Rafez 2003, Bertan et al 2006. As CLGs também são responsáveis pela síntese de ocitocina em vacas (Fehr et al 1987). A partir desses aspectos é possível que as alterações gonadais decorrentes dos APs presentes no Senecio possam causar interferências nesses mecanismos e promover déficits reprodutivos em vacas.…”

Section: Resultsunclassified

Megalocitose de células luteínicas grandes de vacas prenhes com seneciose crônica

et al. 2015

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Diversas espécies de Senecio estão amplamente difundidas nas pastagens de propriedades rurais do Sul do Brasil. Criadores dessa região relatam quedas nos índices reprodutivos dos rebanhos bovinos, muitas vezes de causas não determinadas. Várias plantas tóxicas são capazes de causar alterações reprodutivas diretas e indiretas em bovinos em diversos países, incluindo o Brasil, no entanto seus mecanismos patogenéticos ainda são pouco compreendidos. O objetivo desse trabalho é descrever lesões ovarianas em vacas com seneciose crônica proveniente de propriedades rurais da mesorregião Sudoeste Rio-grandense. Foram estudados 21 casos positivos de seneciose crônica diagnosticados entre 2011 e 2014. O estudo revelou que a seneciose crônica é a principal causa de morte de bovinos adultos na região. Quatro vacas prenhes apresentaram lesões hepáticas clássicas da intoxicação por Senecio spp. Essas vacas tiveram seus ovários avaliados histologicamente e células luteínicas grandes (CLG) desses ovários apresentavam megalocitose e pseudoinclusões nucleares. Algumas CLG apresentaram núcleos com até 23,69μm de diâmetro e o aumento no tamanho desses núcleos foi significativamente maior que os de vacas controle. Conclui-se que a intoxicação por Senecio spp. causa alterações ovarianas em vacas e é possível que a intoxicação cause perdas reprodutivas nos rebanhos bovinas da região.

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“…In sheep, it is highest on day 8 and lowest on day 4; however, the concentration varied as a function of the estrous cycle [31]. OT is produced and stored in the granulosa cell-derived large luteal cells as shown in many papers [4][5][6]. In addition, the large steroidogenic luteal cells in the ewe, which synthesize and secrete OT, contain a markedly higher concentration of cytosolic E 2 receptors than the small luteal cells [31].…”

Section: Discussionmentioning

confidence: 99%

Effects of Estradiol-17.BETA. on Oxytocin and Progesterone Release of the Bovine Corpus Luteum during the Estrous Cycle.

Liebermann

Schams

1995

Journal of Reproduction and Development

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Expression of the oxytocin gene in the large cells of the bovine corpus luteum

Cited by 66 publications

References 15 publications

Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons

Expression of the vasopressin and oxytocin genes in rats occurs in mutually exclusive sets of hypothalamic neurons

Megalocitose de células luteínicas grandes de vacas prenhes com seneciose crônica

Effects of Estradiol-17.BETA. on Oxytocin and Progesterone Release of the Bovine Corpus Luteum during the Estrous Cycle.

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