Expression of the pyrroline-5-carboxylate reductase (P5CR) gene from the wild grapevine Vitis yeshanensis promotes drought resistance in transgenic Arabidopsis

Chen, Chengcheng; Cui, Xiaoyue; Zhang, Pingying; Wang, Zheng; Zhang, Jianxia

doi:10.1016/j.plaphy.2021.10.004

Cited by 27 publications

(8 citation statements)

References 87 publications

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“…Pyrroline-5-carboxylic acid reductase (P5CR) is a terminal enzyme that functions critically in proline biosynthesis 45 . To improve their salt tolerance, the leaves of Sorghum bicolor 46 and sweet potato 47 can accumulate large amounts of proline via overexpression of the P5CR protein under salt stress.…”

Section: Discussionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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Soil salinity can severely restrict plant growth. Yet Reaumuria soongorica can tolerate salinity well. However, large-scale proteomic studies of this plant’s response to salinity have yet to reported. Here, R. soongorica seedlings (4 months old) were used in an experiment where NaCl solutions simulated levels of soil salinity stress. The fresh weight, root/shoot ratio, leaf relative conductivity, proline content, and total leaf area of R. soongorica under CK (0 mM NaCl), low (200 mM NaCl), and high (500 mM NaCl) salt stress were determined. The results showed that the proline content of leaves was positively correlated with salt concentration. With greater salinity, the plant fresh weight, root/shoot ratio, and total leaf area increased initially but then decreased, and vice-versa for the relative electrical conductivity of leaves. Using iTRAQ proteomic sequencing, 47 177 136 differentially expressed proteins (DEPs) were identified in low-salt versus CK, high-salt versus control, and high-salt versus low-salt comparisons, respectively. A total of 72 DEPs were further screened from the comparison groupings, of which 34 DEPs increased and 38 DEPs decreased in abundance. These DEPs are mainly involved in translation, ribosomal structure, and biogenesis. Finally, 21 key DEPs (SCORE value ≥ 60 points) were identified as potential targets for salt tolerance of R. soongolica. By comparing the protein structure of treated versus CK leaves under salt stress, we revealed the key candidate genes underpinning R. soongolica’s salt tolerance ability. This works provides fresh insight into its physiological adaptation strategy and molecular regulatory network, and a molecular basis for enhancing its breeding, under salt stress conditions.

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Section: Discussionmentioning

confidence: 99%

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Yan

Chong

Ming

et al. 2022

Sci Rep

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“…TaPUB15 overexpression resulted in enhanced root growth and development and an increased resistance to salt stress in rice [37]. E3 ubiquitin ligase functions in response to abiotic stress and is dependent on the ABA pathway [38]. Similarly, TaPUB1 and TaPUB15 E3 ligases were shown to be highly expressed in transgenic plants in response to ABA signaling and enhance tolerance to salt stress [13,37].…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Kim

et al. 2021

IJMS

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Plant U-box E3 ubiquitin ligase (PUB) is involved in various environmental stress conditions. However, the molecular mechanism of U-box proteins in response to abiotic stress in wheat remains unknown. In this study, two U-box E3 ligase genes (TaPUB2 and TaPUB3), which are highly expressed in response to adverse abiotic stresses, were isolated from common wheat, and their cellular functions were characterized under drought stress. Transient expression assay revealed that TaPUB2 was localized in the cytoplasm and Golgi apparatus, whereas TaPUB3 was expressed only in the Golgi apparatus in wheat protoplasts. Additionally, TaPUB2 and TaPUB3 underwent self-ubiquitination. Moreover, TaPUB2/TaPUB3 heterodimer was identified in yeast and the cytoplasm of wheat protoplasts using a pull-down assay and bimolecular fluorescence complementation analysis. Heterogeneous overexpression of TaPUB2 and TaPUB3 conferred tolerance to drought stress. Taken together, these results implied that the heterodimeric form of U-box E3 ubiquitin ligases (TaPUB2/TaPUB3) responded to abiotic stress and roles as a positive regulator of drought stress tolerance.

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“…RD29A , COR47 and COR15A genes are responsive to drought in Arabidopsis [ 44 ]. The increased drought tolerance in VvNAC08 -OE [ 45 ] and VyP5CR -OE transgenic lines [ 46 ] and ataf1-1 , ataf1-2 mutants of A. thaliana [ 47 ] is partly associated with higher transcription levels of drought stress-responsive genes RD29A , COR47 and COR15A . Higher transcript levels of COR15A and COR47 were maintained in three SgDREB2C -overexpressing lines and higher levels of RD29A transcript were maintained in two lines compared with WT plants under drought stress.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of SgDREB2C from Stylosanthes guianensis Leads to Increased Drought Tolerance in Transgenic Arabidopsis

Han

Xiang

Song

et al. 2022

IJMS

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Stylosanthes guianensis is an excellent forage legume in subtropical and tropical regions with drought tolerance, but little is known about its drought tolerance mechanism. Dehydration responsive element binding proteins (DREBs) are responsive to abiotic stresses. A SgDREB2C was cloned from S. guianensis, while SgDREB2C protein was localized at nucleus. SgDREB2C transcript was induced by dehydration treatment. Transgenic Arabidopsis overexpressing SgDREB2C showed enhanced osmotic and drought tolerance with higher levels of relative germination rate, seedlings survival rate and Fv/Fm and lower levels of ion leakage compared with WT after osmotic and drought stress treatments. In addition, higher levels of superoxide dismutase (SOD) and ascorbate peroxidase (APX) activities and stress responsive gene (COR15A, COR47) transcripts were observed in transgenic Arabidopsis than in WT under drought stress. These results suggest that SgDREB2C regulated drought tolerance, which was associated with increased SOD and APX activities and stress-responsive gene expression under drought stress.

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Expression of the pyrroline-5-carboxylate reductase (P5CR) gene from the wild grapevine Vitis yeshanensis promotes drought resistance in transgenic Arabidopsis

Cited by 27 publications

References 87 publications

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Physiological response and proteomics analysis of Reaumuria soongorica under salt stress

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Overexpression of SgDREB2C from Stylosanthes guianensis Leads to Increased Drought Tolerance in Transgenic Arabidopsis

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