Expression of the ribosomal proteins Rplp0, Rplp1, and Rplp2 in gynecologic tumors

Proteome changes induced by overexpression of the p75 neurotrophin receptor (p75NTR) in breast cancer cells

“…Analysis by RT-PCR of a set of breast tumor mRNA extracts has shown that other genes are statistically more stable than RPLP0. Also, RPLP0 mRNA was found to be up-regulated in ovarian and endometrial tumors compared to control tissues (Artero-Castro et al, 2011). To our knowledge, we are the first to show an upregulation of RPLP0 under stress induced by a pro-apoptotic agent, reinforcing the idea that RPLP0 should be used with caution as a reference for gene expression in cancers.…”

Disruption of the ribosomal P complex leads to stress-induced autophagy

“…In support of this concept, we have previously reported that RPLP proteins seem to play an important role in breast cancer, which agrees with previous research from other research groups concerning RPLP proteins in tumorigenesis. 15,16,[64][65][66] The fact that the overexpression of RPLP proteins in cancer can be potentially related to a defect in autophagy suggests that translational approaches of autophagy-based therapies should be considered. For example, it would be very interesting to explore if the antiproliferative effect of gonadotropin-releasing hormone analogs that inhibit RPLP1 and RPLP2 is linked to an autophagic response.…”

“…15 To examine whether the downregulation of RPLP proteins has the converse effect (i.e., prevents cancer cell growth), we used cancer cell lines of breast (MCF-7 and MDA-MB-231), colon (HCT116 TP53 ¡/¡ and HT-29), and ovarian carcinoma (OV-90). All siRNAs tested targeting RPLP0, RPLP1, or RPLP2 genes were able to inhibit the corresponding protein by >80% (Fig.…”

“…We have also found positive correlations between the expression of RPLP proteins and the presence of metastasis in different subtypes of gynecological cancer. 15 Despite mounting evidence of RPLP protein overexpression in cancer cells and a link between their downregulation and specific drug responses, 16 it remains unknown how RPLP proteins contribute to these specific cellular changes in human tumors. In the present study, we inhibited the P complex in cancer cells and studied the underlying molecular events that are directly associated with RPLP protein downregulation, including their potential regulatory role in cell cycle arrest and their ability to induce autophagy.…”

Proteomic analysis of porcine alveolar macrophages infected with porcine circovirus type 2

“…ARPP0 is related to protein synthesis [41], cell proliferation and cell survival [42], and gynecologic tumor progression [43]. PRP19 is required for the activation of the pre-mRNA spliceosome and for the stable association of the small nuclear RNAs U5 and U6 [44,45].…”