Expression of Three Mouse Homologs of the Drosophila Segment Polarity Gene cubitus interruptus, Gli, Gli-2, and Gli-3, in Ectoderm- and Mesoderm-Derived Tissues Suggests Multiple Roles during Postimplantation Development

Hui, Chi Chung; Slusarski, Diane C.; Platt, Kenneth A.; Holmgren, Robert A.; Joyner, Alexandra L.

doi:10.1006/dbio.1994.1097

Cited by 446 publications

(301 citation statements)

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“…The sizes of the Gli and Gli3 transcripts detected in spermatogonia were identical to those in mouse embryos (&4 kb and 9 kb, respectively). The fact that two distinct Gli transcripts (4 and 4.4 kb) were not resolved, as previously reported for mouse embryos (Hui et al, 1994), likely re¯ects band compression due to adjacent 28S ribosomal RNA in our experiments. The expression pattern for Gli and Gli3 during spermatogenesis is consistent with their very low transcript levels in adult mouse testis (Figure 1), since spermatogonial cells are present in much smaller numbers than later spermatogenic stages in this tissue.…”

Section: Expression Of Gli Genes During Spermatogenesissupporting

confidence: 77%

“…The present ®ndings thus extend the implicated role for Gli genes in developing cells to spermatogenesis as well. The selective association of Gli family gene expression with mitotically active male germ cells is similar to the situation during somatic cell development, in which all three Gli genes are expressed in actively dividing mesodermal and ectodermal cells, but are not detectable in post-mitotic cells (Hui et al, 1994). One important di erence, however, is the absence of signi®cant Gli2 expression during development of the male germ line, which appears to be a predominant Gli family member in day 14 mouse embryos (see Figure 1).…”

Section: Discussionmentioning

confidence: 57%

“…This suggests either a unique function for Gli2 in somatic cell development and/or redundancy in Gli family activities during mitosis of spermatogenic cells. It is noteworthy that in the developing neural tube Gli2 and Gli3 exhibit overlapping patterns of expression in ventral regions while Gli expression is mainly dorsal (Hui et al, 1994). Figure 5 Developmental expression of testicular Gli DNA binding proteins.…”

Section: Discussionmentioning

confidence: 99%

“…The Gli genes constitute a family of DNA binding proteins that have been implicated in several neoplasias, including glioblastoma (Kinzler et al, 1987;Roberts et al, 1989). Three distinct Gli genes have been identi®ed in human and mouse, Gli, Gli2, Gli3 Ruppert et al, 1988Ruppert et al, , 1990Vortkamp et al, 1992;Hui et al, 1994). The Gli protein has been shown to transform cells in combination with the adenovirus E1A protein (Ruppert et al, 1991), consistent with its presumptive role as an oncogene.…”

Section: Introductionmentioning

confidence: 95%

“…The identi®cation of the Gli homologues ci (Orenic et al, 1990) and tra-1 (Zarkower and Hodgkin, 1993), which regulate development in Drosophila and Caenorhabditis elegans, respectively, has provided additional support for this notion. Further, more recent studies have shown that all three mouse Gli genes are selectively expressed in developing mesodermal and ectodermal tissues (Hui et al, 1994;Walterhouse et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

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Gli family members are differentially expressed during the mitotic phase of spermatogenesis

et al. 1997

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The Gli family of DNA binding proteins has been implicated in multiple neoplasias and developmental abnormalities, suggesting a primary involvement in cell development and di erentiation. However, to date their speci®c roles and mechanisms of action remain obscure, and a drawback has been the lack of a model system in which to study their normal function. Here we demonstrate that Gli family members are di erentially expressed during spermatogenesis in mice. Speci®cally, Gli and Gli3 mRNAs were detected in mouse germ cells, while Gli2 was not. Further, both Gli and Gli3 exhibited stage-dependent patterns of expression selectively in type A and B spermatogonia. Gli expression was somewhat higher in type B spermatogonia while the abundance of Gli3 transcripts was similar in type A and B cells. Gel-shift analyses also demonstrated the enrichment of DNA binding activity speci®c for the Gli target sequence in spermatogonial cells. These results indicate a selective role for Gli and Gli3 during mitotic stages of male germ cell development. Spermatogenesis may thus provide a unique opportunity to identify downstream targets and explore the normal function of Gli family proteins.

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Section: Expression Of Gli Genes During Spermatogenesissupporting

confidence: 77%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Gli family members are differentially expressed during the mitotic phase of spermatogenesis

et al. 1997

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Synteny-defined candidate genes for congenital and idiopathic scoliosis

1999

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Idiopathic scoliosis (IS) is a common but poorly understood syndrome. Congenital scoliosis (CS) is less common but comparably unexplored. Previous studies suggest that each has a significant genetic component. However, the occurrence of scoliosis in the presence of other hereditary connective tissue syndromes raises the possibility that IS and CS are in fact a heterogeneous group of disorders with varied pathogenetic mechanisms. Mouse mutations have proven informative in identifying genes that are important in the development of the musculoskeletal system and provided important mechanistic insights regarding their roles in human disease. We sought to identify candidate genes for human IS and CS by reviewing mouse mutations with phenotypes affecting the axial skeleton. We performed a systematic review using the Mouse Genome Database (MGD), the Genome Database (GDB), and the Online Mendelian Inheritance in Man (OMIM) world-wide-web sites with additional searches performed based on the results of this initial search. We identified approximately 400 mouse mutations, reviewed approximately 250 of these for vertebral phenotypes, assessed 45 of these for synteny conservation between mouse and man, and identified 28 mouse mutations for which 29 credible candidates for human scoliosis could be identified based on mouse phenotypic and mapping data. For each of these, we have synthesized information about the mouse mutant phenotype, mapping data, information regarding molecular pathogenesis when a specific causative gene has been identified, and information regarding plausible candidates based on map position when the causative gene has not been identified. Among these were three loci for which the mutant gene had been identified and the human homologue was known. Some of the mouse mutants have phenotypes similar to human syndromes.

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Expression ofSonic hedgehog,Patched, andGli1 in developing taste papillae of the mouse

1999

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Expression of Three Mouse Homologs of the Drosophila Segment Polarity Gene cubitus interruptus, Gli, Gli-2, and Gli-3, in Ectoderm- and Mesoderm-Derived Tissues Suggests Multiple Roles during Postimplantation Development

Cited by 446 publications

References 0 publications

Gli family members are differentially expressed during the mitotic phase of spermatogenesis

Gli family members are differentially expressed during the mitotic phase of spermatogenesis

Synteny-defined candidate genes for congenital and idiopathic scoliosis

Expression ofSonic hedgehog,Patched, andGli1 in developing taste papillae of the mouse

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