Expression of Tumor Necrosis Factor-α, Interleukin-1α, Interleukin-6 and Interleukin-10 in Chronic Otitis Media with Bone Osteolysis

Kuczkowski, Jerzy; Sakowicz-Burkiewicz, Monika; Iżycka‐Świeszewska, Ewa; Mikaszewski, Bogusław; Pawełczyk, Tadeusz

doi:10.1159/000323831

Cited by 43 publications

(48 citation statements)

References 35 publications

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“…In contrast, the pro-inflammatory cytokines TNF-α, IL-1β and IL-6 were detected, with their secretion being elevated upon stimulation of the cells with both UHMWPE particles and LPS in a time-dependent manner. It is well known that these cytokines are involved in the development of the condition of periprosthetic osteolysis [53]. Thereby, TNF-α seems to play a leading role as it not only promotes bone resorption, but also impairs bone formation by downregulating the gene expression of the extracellular matrix proteins collagen, osteocalcin, alkaline phosphatase and the transcription factor Runx in osteoblasts [54].…”

Section: Discussionmentioning

confidence: 99%

Calcitonin Gene-Related Peptide Modulates the Production of Pro-Inflammatory Cytokines Associated with Periprosthetic Osteolysis by THP-1 Macrophage-Like Cells

Jablonski¹,

Kauther

Bachmann

et al. 2014

Neuroimmunomodulation

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Objective: An anti-resorptive impact of the neuropeptide calcitonin gene-related peptide (CGRP) on periprosthetic osteolysis, the leading cause of early prosthesis loosening, has been shown previously. In this study, the impact of CGRP on pro-inflammatory cytokine production associated with periprosthetic osteolysis was analysed using THP-1 macrophage-like cells. Methods: Cells were stimulated with ultra-high-molecular-weight polyethylene (UHMWPE) particles (cell-to-particle ratios of 1:100 and 1:500) and lipopolysaccharides (LPS; 1 µg/ml) to establish osteolytic conditions, and simultaneously treated with CGRP (10^-8M). Receptor activator of nuclear factor-κB (RANK), RANK ligand (RANKL) and tumour necrosis factor (TNF)-a mRNA expression were measured by quantitative RT-PCR. RANK protein was detected by Western blot. Secreted protein levels of TNF-a as well as interleukin (IL)-1ß and IL-6 were quantified in cell culture supernatants by ELISA and Bio-Plex cytokine assay, respectively. Results: Activation of macrophage-like cells failed to enhance the production of RANK but led to a dose- and time-dependent increase of TNF-a mRNA and secreted protein levels of TNF-a, IL-1ß and IL-6. Application of CGRP time-dependently suppressed TNF-a mRNA expression induced by low-particle concentrations and LPS, while both particle- and LPS-induced secretion of TNF-a was inhibited. A pronounced inhibitory effect of CGRP on LPS-induced cytokine production at 24 h of incubation was also observed with IL-1ß and IL-6. Conclusions: CGRP shows a time-dependent inhibitory effect on the secretion of osteolysis-associated pro-inflammatory cytokines, indicating an indirect anti-resorptive influence of the neuropeptide on both aseptic prosthesis loosening and bacterially induced bone resorption which might enhance the life time of total joint replacements.

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Section: Discussionmentioning

confidence: 99%

Calcitonin Gene-Related Peptide Modulates the Production of Pro-Inflammatory Cytokines Associated with Periprosthetic Osteolysis by THP-1 Macrophage-Like Cells

Jablonski¹,

Kauther

Bachmann

et al. 2014

Neuroimmunomodulation

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“…It has been suggested that mast cells may be involved in the bone resorption observed in CSOM. 138,139 Bone resorption is the process by which osteoclasts break down bone and release minerals, resulting in a transfer of calcium from bone fluid to the blood. It is an important aspect of CSOM contributing to many complications of this disease such as ossicular erosion.…”

Section: Mast Cellsmentioning

confidence: 99%

Role of innate immunity in the pathogenesis of otitis media

Mittal

Kodiyan

Gerring

et al. 2014

International Journal of Infectious Diseases

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Summary Otitis media (OM) is a public health problem in both developed and developing countries. It is the leading cause of hearing loss and represents a significant healthcare burden. In some cases, acute OM progresses to chronic suppurative OM (CSOM), characterized by effusion and discharge, despite antimicrobial therapy. The emergence of antibiotic resistance and potential ototoxicity of antibiotics has created an urgent need to design non-conventional therapeutic strategies against OM based on modern insights into its pathophysiology. In this article, we review the role of innate immunity as it pertains to OM and discuss recent advances in understanding the role of innate immune cells in protecting the middle ear. We also discuss the mechanisms utilized by pathogens to subvert innate immunity and thereby overcome defensive responses. A better knowledge about bacterial virulence and host resistance promises to reveal novel targets to design effective treatment strategies against OM. The identification and characterization of small natural compounds that can boost innate immunity may provide new avenues for the treatment of OM. There is also a need to design novel methods for targeted delivery of these compounds into the middle ear, allowing higher therapeutic doses and minimizing systemic side effects.

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“…Second, local immunity in middle ear may act in a different manner contrary to systemic immunity affected by obesity. Third, methodologically, transcription level used in this study does not always correlated with protein level and cytokine expression is regulated both at the transcriptional level and translational mechanisms [20]. However this assumption needs further verification.…”

Section: Discussionmentioning

confidence: 98%

Decreased Pattern-Recognition Receptor-Mediated Cytokine mRNA Expression in Obese Children With Otitis Media With Effusion

Kim

Lee

et al. 2014

Clin Exp Otorhinolaryngol

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ObjectivesTo assess innate and humoral immune responses in middle ear effusion of obese pediatric patients with otitis media with effusion (OME).MethodsWe evaluated 219 children with OME, of whom 21 were obese and 198 were non-obese. We compared the expression in middle ear effusion of mRNAs encoding toll-like receptors (TLR) 2, 4, 5, and 9; nucleotide-binding oligomerization domains (NOD) 1 and 2; retinoic acid-inducible gene (RIG)-I; interleukins (IL)-6, -10, and -12; interferon (IFN)-γ; and tumor necrosis factor (TNF)-α mRNAs. We also compared the expression of immunoglobulins IgG, IgA, and IgM and the bacterial detection rate in the two groups.ResultsTLR2-mediated expression of IL-6 mRNA, TLR4-mediated expression of IL-6 and IL-10 mRNA, TLR5-mediated expression of IL-6, IL-10, and TNF-α mRNA, TLR9-mediated expression of IL-6 mRNA, and NOD2-mediated expression of IL-6, IL-12, and TNF-α mRNA were significantly lower in obese than in non-obese children (P<0.05). However, concentrations of IgG, IgA, and IgM in middle ear effusion were lower in obese than in non-obese children, but none of these differences was significant (P>0.05).ConclusionMean body mass index was higher and pattern-recognition receptor-mediated cytokine mRNA expression was lower in obese than in non-obese children with OME.

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Expression of Tumor Necrosis Factor-α, Interleukin-1α, Interleukin-6 and Interleukin-10 in Chronic Otitis Media with Bone Osteolysis

Cited by 43 publications

References 35 publications

Calcitonin Gene-Related Peptide Modulates the Production of Pro-Inflammatory Cytokines Associated with Periprosthetic Osteolysis by THP-1 Macrophage-Like Cells

Calcitonin Gene-Related Peptide Modulates the Production of Pro-Inflammatory Cytokines Associated with Periprosthetic Osteolysis by THP-1 Macrophage-Like Cells

Role of innate immunity in the pathogenesis of otitis media

Decreased Pattern-Recognition Receptor-Mediated Cytokine mRNA Expression in Obese Children With Otitis Media With Effusion

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