Expression of two soybean resistance gene candidates shows divergence of paralogous single-copy genes

Hayes, A. J.; Yue, Yang; Maroof, M. A. Saghai

doi:10.1007/s001220051545

Cited by 17 publications

(13 citation statements)

References 23 publications

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“…Several homozygous strated that soybean carries numerous duplicate loci on homeologous chromosomes, lending credence to its recombinants developed in this study carry 3gG2 along with other subclass G genes derived from the susceptible purported ancient tetraploid origin (Shoemaker et al 1996). For example, the soybean resistance gene candisystem, we observe that new arrangements of gene family members interact in unique ways to condition novel date, L20a, which also resides on MLG F near Rsv1, has a paralog on the soybean MLG E (Hayes et al 2000).…”

Section: Disease Screening Of Homozygous Recombinantsmentioning

confidence: 99%

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Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

et al. 2004

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The soybean Rsv1 gene for resistance to soybean mosaic virus (SMV; Potyvirus) has previously been described as a single-locus multi-allelic gene mapping to molecular linkage group (MLG) F. Various Rsv1 alleles condition different responses to the seven (G1-G7) described strains of SMV, including extreme resistance, localized and systemic necrosis, and mosaic symptoms. We describe the cloning of a cluster of NBS-LRR resistance gene candidates from MLG F of the virus-resistant soybean line PI96983 and demonstrate that multiple genes within this cluster interact to condition unique responses to SMV strains. In addition to cloning 3gG2, a strong candidate for the major Rsv1 resistance gene from PI96983, we describe various unique resistant and necrotic reactions coincident with the presence or absence of other members of this gene cluster. Responses of recombinant lines from a high-resolution mapping population of PI96983 (resistant) ϫ Lee 68 (susceptible) demonstrate that more than one gene in this region of the PI96983 chromosome conditions resistance and/or necrosis to SMV. In addition, the soybean cultivars Marshall and Ogden, which carry other previously described Rsv1 alleles, are shown to possess the 3gG2 gene in a NBS-LRR gene cluster background distinct from PI96983. These observations suggest that two or more related non-TIR-NBS-LRR gene products are likely involved in the allelic response of several Rsv1-containing lines to SMV.

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Section: Disease Screening Of Homozygous Recombinantsmentioning

confidence: 99%

“…Southern blotting was (Rsv1) ϫ Lee 68 (rsv1) was used for mapping and identificaperformed as described in Hayes et al (2000). tion of recombinant lines.…”

mentioning

confidence: 99%

Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

et al. 2004

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“…We also compared soybean cv Williams 82 with a second accession of soybean, PI96983, which was selected because it differs from Williams 82 functionally at several disease resistance loci mapped to the Rpg1 region (Ashfield et al, 1998;Hayes et al, 2000;Jeong et al, 2001;Gore et al, 2002). A study of singlenucleotide polymorphisms in soybean, however, has shown that domesticated soybean has undergone a significant genetic bottleneck, with total variation at the single-nucleotide polymorphism level being much lower than that observed in maize (Hyten et al, 2006).…”

Section: Differences Between Soybean Accessions Pi96983 and Williams mentioning

confidence: 99%

Differential Accumulation of Retroelements and Diversification of NB-LRR Disease Resistance Genes in Duplicated Regions following Polyploidy in the Ancestor of Soybean

et al. 2008

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The genomes of most, if not all, flowering plants have undergone whole genome duplication events during their evolution. The impact of such polyploidy events is poorly understood, as is the fate of most duplicated genes. We sequenced an approximately 1 million-bp region in soybean (Glycine max) centered on the Rpg1-b disease resistance gene and compared this region with a region duplicated 10 to 14 million years ago. These two regions were also compared with homologous regions in several related legume species (a second soybean genotype, Glycine tomentella, Phaseolus vulgaris, and Medicago truncatula), which enabled us to determine how each of the duplicated regions (homoeologues) in soybean has changed following polyploidy. The biggest change was in retroelement content, with homoeologue 2 having expanded to 3-fold the size of homoeologue 1. Despite this accumulation of retroelements, over 77% of the duplicated low-copy genes have been retained in the same order and appear to be functional. This finding contrasts with recent analyses of the maize (Zea mays) genome, in which only about one-third of duplicated genes appear to have been retained over a similar time period. Fluorescent in situ hybridization revealed that the homoeologue 2 region is located very near a centromere. Thus, pericentromeric localization, per se, does not result in a high rate of gene inactivation, despite greatly accelerated retrotransposon accumulation. In contrast to low-copy genes, nucleotide-binding-leucine-rich repeat disease resistance gene clusters have undergone dramatic species/homoeologuespecific duplications and losses, with some evidence for partitioning of subfamilies between homoeologues.The comparative approach to studying genes and genomes is a powerful method for addressing both

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“…This homology is supported by Shoemaker et al (1996) who reported that an extensive region of homology on soybean chromosomes 13 and 15 exist. Hayes et al (2000) also reported that two NBS-LRR classes of disease-resistance genes identified by the same probe were mapped on soybean chromosome 13 and 15 and these genes have strong homology. These results suggest that the minor QTL for peanut root-knot nematode resistance on chromosome 15 could be a homolog of the major QTL on chromosome 13.…”

Section: Discussionmentioning

confidence: 90%

Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538

et al. 2010

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Expression of two soybean resistance gene candidates shows divergence of paralogous single-copy genes

Cited by 17 publications

References 23 publications

Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Recombination Within a Nucleotide-Binding-Site/Leucine-Rich-Repeat Gene Cluster Produces New Variants Conditioning Resistance to Soybean Mosaic Virus in Soybeans

Differential Accumulation of Retroelements and Diversification of NB-LRR Disease Resistance Genes in Duplicated Regions following Polyploidy in the Ancestor of Soybean

Fine mapping of the soybean aphid-resistance gene Rag2 in soybean PI 200538

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