Expression of type I collagen in the capsule of a contracture knee in a rat model

Hagiwara, Yoshihiro; Chimoto, E.; Andõ, Akira; Sasano, Yasuyuki; Hatori, Kouki; Itoi, Eiji

doi:10.3109/2000-1967-208

Cited by 12 publications

(18 citation statements)

References 26 publications

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“…Others reported neither collagen type I nor type III of the periarticular connective tissue changed in rat immobilized knee joints induced by spinal cord injury using immunohistochemistry21 or Western blotting 20. In our previous study, collagen type I mRNA expression was decreased only after 3 days of immobilization by in situ hybridization 6. Expression patterns of collagen types I and III in the contracture capsule is remained controversial.…”

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confidence: 76%

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Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Hagiwara

Andõ

Onoda

et al. 2009

Journal Orthopaedic Research

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Our objective was to determine the changes in expression of collagen types I and III in the capsule of a rat knee contracture model. The unilateral knee joints of adult male rats were rigidly immobilized at 150 degrees of flexion using a rigid plastic plate and screws for 3 days, 1, 2, 4, 8, and 16 weeks (immobilized group). Sham-operated animals had holes drilled in the femur and tibia with screws inserted without a plate (control group). The expression patterns of collagen types I and III in the anterior and posterior capsule were evaluated by in situ hybridization (ISH), quantitative real-time polymerase chain reaction (qPCR), immunohistochemistry (IHC), and Western blotting (WB). Expressions of collagen types I and III were decreased after immobilization compared to the control group by ISH and qPCR. The expression was not changed after immobilization compared to the control group by IHC and WB. The expression of mRNA and protein levels of collagen types I and III were not increased after immobilization, which indicated that accumulation of the two types of collagen was not the etiology of joint contracture. Another process, such as capsule and synovial adhesions, may be one possible cause of joint contracture.

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confidence: 76%

“…Once joint contracture is established, it is extremely difficult to regain a full range of motion with vigorous and extensive rehabilitation, or even with surgical treatment 5. As short a period of immobilization as possible is the only way to avoid joint contracture 6…”

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confidence: 99%

Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Hagiwara

Andõ

Onoda

et al. 2009

Journal Orthopaedic Research

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“…The protocol used in the present study has been reported previously (Sasano et al 2001;Hagiwara et al 2007) and is briefly described as follows: (i) the deparaffinized sections were immersed in 0.2 N HCl for 20 minutes and, after washing in PBS, incubated in proteinase K (20 μ g/ml) in PBS for 30 minutes at 37°C; (ii) after washing, the sections were dipped in 100% ethanol and dried in air; (iii) the sections were incubated with the antisense or sense control probes (400-1000 ng/ml) in a hybridization mixture for 16 hours at 45°C…”

Section: In Situ Hybridizationmentioning

confidence: 99%

Increased Expression of Metalloproteinase-8 and -13 on Articular Cartilage in a Rat Immobilized Knee Model

Andõ

Hagiwara

Tsuchiya

et al. 2009

Tohoku J. Exp. Med.

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“…Type I collagen composes approximately 85% of the collagen content of the joint capsule, and provides mechanical strength, contributes to tissue elasticity, and is produced in sites undergoing fibrosis. 90 In another study, the collagen of control and immobilized It is a constituent of normal synovial tissue, and is also present in inflamed and proliferative rheumatoid synovium.…”

Section: Effects Of Medications On Immobilized Cartilagementioning

confidence: 96%

Responses of Musculoskeletal Tissues to Disuse and Remobilization

Millis¹

2014

Canine Rehabilitation and Physical Therapy

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P atients afflicted with neurologic, orthopedic, metabolic, and other diseases frequently have reduced weight bearing and use of limbs. In addition, some conditions may be treated with splints, casts, or other coaptation devices, resulting in decreased limb use. The tissues most affected by disuse and immobilization are cartilage, joint capsule, muscle, ligament, tendon, and bone. Knowledge of how these tissues respond to disuse and immobilization, the types of deleterious tissue changes, and the time frame over which these changes occur, are important in understanding the need for physical rehabilitation. For example, stress deprivation of joints results in proliferation of connective tissue within the joint space, adhesions between synovial folds, adherence of connective tissue to cartilage surfaces, atrophy of cartilage, reduced proteoglycan (PG) content, ulceration at points of cartilage to cartilage contact, disorganization of cellular and fibrillar ligament alignment, reduced collagen mass, increased ligament compliance, weakened ligament insertion sites, reduced load-to-failure and energy-absorbing capacity of the bone-ligament-bone complex, and osteoporosis of the involved extremity. 1 Rehabilitation of patients with acute or chronic neurologic or orthopedic conditions involves the application of controlled challenges to tissues to improve strength, condition, and function. It is important to understand how to safely remobilize tissues after injury and a period of immobilization. Rehabilitation must sufficiently challenge tissues to enhance and positively influence their recovery and healing. However, if tissues are overchallenged, they may be damaged, ultimately delaying recovery or cause additional injury. There must be a balance between the simultaneous demands for protection against undue stress to facilitate healing, and the need for stress to attenuate atrophy of musculoskeletal tissues. 2 A review of these concepts forms the basis for a rational approach to developing rehabilitation programs. CartilageDisorders of articular cartilage and joints are some of the most common and debilitating diseases encountered in veterinary practice. Understanding the normal structure and function of articular cartilage is a prerequisite to understanding the pathologic processes. The mechanical properties of articular cartilage arise from the complex structure and interactions of its biochemical constituents. The viscoelastic properties of cartilage, primarily resulting from fluid flow through the solid matrix, explain deformational responses observed during loading. For example, cartilage that is loaded rapidly is stiffer than the same cartilage that is loaded slowly. Degenerative processes include the breakdown of normal constituents of cartilage, especially the collagen network and PGs, which affects the mechanics of fluid flow through cartilage. Factors contributing to such breakdown include direct trauma, obesity, immobilization, and excessive repetitive loading of the cartilage. Training activities, without tr...

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Expression of type I collagen in the capsule of a contracture knee in a rat model

Cited by 12 publications

References 26 publications

Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Expression patterns of collagen types I and III in the capsule of a rat knee contracture model

Increased Expression of Metalloproteinase-8 and -13 on Articular Cartilage in a Rat Immobilized Knee Model

Responses of Musculoskeletal Tissues to Disuse and Remobilization

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