Expression of vascular endothelial growth factor (VEGF) transcript and protein in the testis of several vertebrates, including endangered species

Reddy, Niranjan; Kasukurthi, Kiran Babu; Mahla, Ranjeet Singh; Pawar, Rahul Mohanchandra; Goel, Sandeep

doi:10.1016/j.theriogenology.2011.08.037

Cited by 15 publications

(18 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, similar to results obtained for the testes of the roe-deer (19), in the present study, a positive immunoreaction for VEGF was also observed in the cytoplasm of the spermatogonia. On the contrary to the case in mice (12) and similar to the case in humans (7), rats (1,23), roe-deer (19) and some wild animals (17), in the rabbit testes, a strong immunopositive reaction was detected for VEGF in the Leydig cells. Furthermore, in agreement with previous reports for rats (1,23) and mice (12), the present study demonstrated VEGF immunoreactivity in the acrosome of the spermatids at stages V, VI, VII, and VIII of spermatogenic development in the rabbit testes.…”

Section: Discussionmentioning

confidence: 80%

“…Schön et al (19) determined that, in roe-deer testes, while positive VEGF immunoreactivity was present in the spermatogonia, spermatocytes and Leydig cells, immunostainings for flt-1 were observed in spermatids and blood vessels, and immunostainings for flk-1 were observed in only the Sertoli cells. In their research on the testes of various wild animals, Reddy et al (17) detected that, VEGF was generally expressed in the Sertoli, Leydig and germ cells. In agreement with findings reported for humans (7), rats (1,23), mice (12) and several wild animals (17), in the present study, a positive immunoreactivity was observed for the VEGF protein in the cytoplasm of the Sertoli cells in the rabbit testicular tissue.…”

Section: Discussionmentioning

confidence: 99%

“…In their research on the testes of various wild animals, Reddy et al (17) detected that, VEGF was generally expressed in the Sertoli, Leydig and germ cells. In agreement with findings reported for humans (7), rats (1,23), mice (12) and several wild animals (17), in the present study, a positive immunoreactivity was observed for the VEGF protein in the cytoplasm of the Sertoli cells in the rabbit testicular tissue. Furthermore, similar to results obtained for the testes of the roe-deer (19), in the present study, a positive immunoreaction for VEGF was also observed in the cytoplasm of the spermatogonia.…”

Section: Discussionmentioning

confidence: 99%

“…Nonetheless, to date, the expression and localization of VEGF and its receptors in the testes and epididymis have been demonstrated in humans (7,8), rats (1,23), mice (12), roe-deer (19) and some wild animals (17) using various methods. In view of the results of these studies, it is considered that VEGF could have an important role in spermatogenesis, spermiogenesis, and steroidogenesis in the testicular tissue (1,7,17,19). On the other hand, to the authors' knowledge, there is no previous study on the presence or localization of the VEGF protein and the flt-1 and flk-1 receptors this protein binds to in the testes and epididymis of the rabbit.…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, to the authors' knowledge, there is no previous study on the presence or localization of the VEGF protein and the flt-1 and flk-1 receptors this protein binds to in the testes and epididymis of the rabbit. This study was based on the hypothesis that, similar to the case in humans (7,8) and some other animal species (1,12,17,19), VEGF and its receptors flt-1 and flk-1 may have an important role in spermatogenesis, spermiogenesis, steroidogenesis and sperm maturation in the testes and epididymis of the rabbit. In this context, this study was aimed at demonstrating the presence and immunohistochemical expression of the VEGF protein and its receptors, flt-1 and flk-1, in the rabbit testes and epididymis by means of the streptavidin-biotin complex (ABC) immunohistochemical technique, and investigating the potential role of these molecules in the physiology of the testes and epididymis.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The immunohistochemical localization of the vascular endothelial growth factor and its receptors in the testes and epididymis of the rabbit

BEYAZ¹

2017

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Summary:This study was aimed at demonstrating the immunohistochemical expression of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis of the rabbit and at investigating the potential role of these molecules in testicular and epididymal physiology. The testes and epididymis of 15 male adult New Zealand rabbits constituted the material of the study. The streptavidinbiotin complex (ABC) immunohistochemical technique was used to determine the localization of VEGF and its receptors flt-1 and flk-1 in the testes and epididymis. In the testes, immunoreactivity for VEGF and its receptors flt-1 and flk-1 was observed in the Sertoli cells and spermatogonia in the seminiferous tubules throughout all of the spermatogenic stages from I to VIII and in the Leydig cells of the interstitium. Furthermore, immunoreactivity was also observed in the acrosome of the spermatids in seminiferous tubules for VEGF at stages V, VI, VII and VIII and in the acrosome of the spermatids at stages VII and VIII and the blood vessels of the interstitium for flk-1. Positive immunoreactivity, ranging from strong to weak, was detected for VEGF, flt-1 and flk-1 in the cytoplasm of the principal, apical, basal and clear cells of the epithelium lining the epididymal initial segment and the caput, corpus and cauda epididymidis. The specific localization of the VEGF protein and its receptors flt-1 and flk-1 in the testes of the rabbit suggests that these molecules could have a functional role in several processes, including spermatogonial differentiation, spermiogenesis, acrosome development in spermatids, steroidogenesis in Leydig cells, epididymal sperm maturation, and the maintenance of the bloodepididymis barrier.Keywords: Epididymis, rabbit, testis, VEGF, VEGF receptors. Tavşan testis ve epididimislerinde vasküler endoteliyal büyüme faktörü ve reseptörlerinin immunohistokimyasal lokalizasyonuÖzet: Bu çalışmanın amacı, VEGF ve reseptörleri flt-1 ile flk-1'in tavşan testis ve epididimisindeki immunohistokimyasal ekspresyonlarını belirlemek ve bu moleküllerin testis ve epididimis fizyolojisindeki potansiyel rollerini araştırmaktır. Çalışmada materyal olarak, 15 adet sağlıklı, erkek Yeni Zellanda tavşanından elde edilen testis ve epididimisler kullanıldı. VEGF ve reseptörleri flt-1 ile flk-1'in testis ve epididimisdeki lokalizasyonlarının belirlenmesi amacıyla streptavidin biotin kompleks (ABC) immunohistokimyasal yöntemi kullanıldı. Testiste, I'den VIII'e kadarki tüm spermatojenik evrelerde seminifer tubüllerdeki Sertoli hücreleri ve spermatogonialarda ve intersitisyumda Leydig hücrelerinde VEGF ve reseptörleri flt-1 ile flk-1 için immunreaktivite gözlendi. Buna ilaveten, seminifer tubüllerde V., VI., VII. ve VIII. evrelerde VEGF için, VII. ve VIII. spermatojenik evrelerde ise flk-1 için spermatidlerin akrozomlarında ve intersitisyumda kan damarlarında immunreaktivite belirlendi. Epididimiste, başlangıç segmenti, kaput, korpus ve kauda epididimidis epitelindeki prensipal, apikal ve bazal hücreleri ile clear hücrelerinin sitoplazmaların...

show abstract