1983
DOI: 10.1104/pp.73.4.915
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Expression of Zein in Long Term Endosperm Cultures of Maize

Abstract: Continuous cultures, established 10 days after pollination from endosperms of inbred A636 Zea mays (L.) were extracted 21 months later with aqueous ethanol. The solubilized proteins were analyzed by polyacrylamide-sodium dodecyl sulfate gel electrophoresis. Two protein bands co-migrated with zein, the major storage protein of maize. Immunoblotting of the gel followed by incubation of the immobilized proteins with anti-zein IgG provided evidence that the polypeptides were in fact zein. Electron microscopic stud… Show more

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Cited by 27 publications
(17 citation statements)
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“…The lack of susceptibility of the sucrose analog 1 '-deoxy-l '-fluorosucrose (FS') to invertase hydrolysis (12) was exploited to distinguish between uptake of intact sucrose and that of hexoses liberated by cell wall invertase. (2,27) in that the tissue contained starch and zein (data not shown). Cultures increased from 3 to 16 g fresh weight during the culture period.…”
mentioning
confidence: 84%
See 1 more Smart Citation
“…The lack of susceptibility of the sucrose analog 1 '-deoxy-l '-fluorosucrose (FS') to invertase hydrolysis (12) was exploited to distinguish between uptake of intact sucrose and that of hexoses liberated by cell wall invertase. (2,27) in that the tissue contained starch and zein (data not shown). Cultures increased from 3 to 16 g fresh weight during the culture period.…”
mentioning
confidence: 84%
“…The differentiation of the suspension culture cells into storage cells corresponding to the intact endosperm is evidenced by their ability to synthesize and accumulate starch (2), zein (27), and anthocyanins (19). There are also ultrastructural similarities between the cultures and the intact parent tissue (8).…”
mentioning
confidence: 99%
“…Since the first establishment of maize endosperm in vitro cultures, studies of starch, storage protein, and anthocyanin biosynthesis have been reported (Chu and Shannon, 1975;Shimamoto et al, 1983;Racchi and Manzocchi, 1988). However, these studies have provided limited insight into mechanisms in endosperm development.…”
Section: Discussionmentioning
confidence: 99%
“…Although procedures have varied over time, usually, 10 d after pollination (DAP), isolated endosperm has been grown on medium with 3% Suc. Whereas these cultures retained some characteristics of in planta endosperm, including some degree of starch, storage protein, and anthocyanin accumulation (Chu and Shannon, 1975;Shimamoto et al, 1983;Racchi and Manzocchi, 1988), their lack of histological organization has limited their value for developmental biology studies (Felker, 1987).…”
mentioning
confidence: 99%
“…Although several approaches for culturing maize (Zea mays) endosperm in vitro have been reported in the past years (Shimamoto et al, 1983), only recently a novel method developed by Odd-Arne Olsen and colleagues (Gruis et al, 2006) has proven to be successful in retaining endosperm tissue and cell type identity in in vitro conditions. Cultures derived from transgenic maize lines in which endosperm cell types are identified by the activity of specific promoters have shown that aleurone and starchy endosperm cell identity continues to be established in vitro (Gruis et al, 2006).…”
mentioning
confidence: 99%