Expression Pattern and Promoter Analysis of a Eucalyptus grandis Germin-like Gene

Sassaki, Flávio Tetsuo; Bravo, Juliana Pereira; Gonzalez, E. R.; Maia, Ivan de Godoy

doi:10.1007/s11105-014-0734-0

Cited by 12 publications

(8 citation statements)

References 34 publications

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“…Conserved regulatory elements in GLP promoter sequences have been reported to be responsive to environmental stresses and growth factors [21,54]. Here, the promoter analysis was performed for GLP genes whose promoter sequences (–2.0 kb) were available in the RGAP and TAIR genome database (Table S10).…”

Section: Resultsmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Chen

et al. 2016

IJMS

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Previous studies have shown that germin-like proteins (GLPs) are present ubiquitously in rice and Arabidopsis. However, the understanding regarding their role in development and abiotic/biotic stress resistance remains limited. In the present study, we report genome-wide identification, characterisation, subcellular localization, enzyme activity, and expression analysis of the GLP gene family in rice and Arabidopsis to study their functions. In total, 43 and 32 GLPs in the rice and Arabidopsis genome were identified based on a systematic analysis, respectively. The GLP genes were clustered into six clades based on phylogenetic analysis, and many stress and developmental-related cis-elements were detected in promoters of GLP genes. In addition, subcellular location and superoxide dismutase (SOD) analysis demonstrated that the random selected OsGLP genes on chromosomes 8 and 4 of rice were expressed in the cell wall with SOD activity. Overall, our results showed that tandem duplication events, especially the clusters of tandem duplication genes on chromosome 8 in rice, play a major role in expansion of the GLP family and thus increase our understanding of the role of the GLP family in abiotic/biotic stress and development.

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Section: Resultsmentioning

confidence: 99%

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Chen

et al. 2016

IJMS

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“…The assay was conducted according to the modified method of Sassaki et al (2014). Transgenic plants were grown on MS media at controlled condition for 30 days.…”

Section: Circadian Rhythm (Dark/light Assay)mentioning

confidence: 99%

“…Histochemically, its induction pattern is comparable to OsRGLP2, GERB and GERF promoters (Federico et al 2006;Mahmood et al 2013). Leaf vein, midribs and stomata guard cells were most active showing its role in resource allocation and gaseous exchange during wounding (Sassaki et al 2014;Yang et al 2013). In root, xylem and phloem were most active, which beside its role in transportation also act as a strategic checkpoint for resistance against pests and pathogens (Turgeon and Wolf 2009).…”

Section: Wound Inducibilitymentioning

confidence: 99%

Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum

et al. 2019

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Germin (GER) and germin-like protein (GLP) genes play a very important role against various stresses. Promoter analysis provide significant insight into gene's function and regulation. Presently, upstream region (1228 bp) of the OsRGLP1 gene was functionally characterized via heterologous expression. It was fused with the Glucuronidase (GUS) reporter gene and the expression cassette was used to transform Nicotiana tabacum using Agrobacterium-mediated transformation. Transgenic plants were examined via quantitative real-time PCR (qPCR) to analyze its role in wounding, salinity, drought, abscisic acid (ABA) and circadian rhythm. OsRGLP1 was highly induced by ABA and drought by showing 28-and 25-fold changes in GUS mRNA level respectively as compared to wounding (fourfold change) and salinity (threefold change). However, no activity was observed in circadian rhythm. Histochemically, strong GUS activity was observed in leaf veins, midrib, epidermal hair, stomata guard cells, stem cortex, root hairs, xylem and phloem and at cellular level in cell wall, cytoplasm and its periphery. OsRGLP1 promoter can be used to develop agronomically important transgenic plants in future food program. KeywordsRice germin-like proteins • OsRGLP1 • Germin • Germin-like protein • Promoter • GUS • Stress * Muhammad Ilyas

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“…Similarly, using information about putative TFBSs, the role of the 276bp promoter region in tissue-specific expression and development was predicted and verified for HvGERB and HvGERF gene promoters [14]. Likewise, the roles of ZmGLP1 and EgGLP promoters in the control of circadian rhythm-oscillated pattern [15,16], PcGer1 in various hormonal stresses [17], TaGLP3 in powdery mildew [18] and HvGer4c and AtGLP13 in pathogenicity [19,20] were first predicted by TFBSs analysis using various bioinformatics tools and subsequently verified. Similarly, due to the presence of seed-specific TFBSs, the BnGLP gene promoter was used to direct and enhance the accumulation of omega-3 long chain molecules by achieving seed-specific expression in transgenic Arabidopsis [21].…”

Section: Introductionmentioning

confidence: 99%

In silico analysis of transcription factor binding sites in promoters of germin-like protein genes in rice

Ilyas

Naqvi

Mahmood

2016

Arch biol sci (Beogr)

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Germins (GERs) and germin-like proteins (GLPs) play important roles in responses to various stresses; however, their function is still not fully understood. Significant insight into their function can be obtained by analyzing their promoters. In the present study, the 5' upstream promoters (1000 bp) of 43 Asian rice (Oryza sativa var. Japonica) GLP genes were retrieved from the Plant Ensemble, based on the Rice Annotation Project database (RAP-DB). Phylogenetic analysis via MEGA6 showed a narrow genetic background (0.2%) with a Tajima neutrality value (π) of 0.69. Overall, 4234 transcription factor (TF) binding sites (TFBSs) were found on chromosomes 1, 2, 3, 4, 5, 8, 9, 11 and 12 via "MatInspector" from 90 different TF families using a total of 444. Common TFs and DiAlign analyses showed that Arabidopsis homeobox protein (AHBP), MYB-like proteins (MYBL) and vertebrate TATA-box-binding protein (VTBP) were the most abundant, common and evolutionarily conserved elements in the upstream region from 0 to -800. Finding their mutual interaction via Farmworker analysis uncovered three new cis-regulatory modules (VTBP_VTBP, MYBS_MYBS, and AHBP_VTBP), which appear to be decisive for OsGLPs regulation. In silico functional analysis via ModelInspector revealed 77 cis-regulatory modules, each comprised of two elements, among which DOFF_OPAQ_03 and GTBX_MYCL_01 were the most frequent and mostly found on chromosome 8 and 12, indicating that the combinatorial interaction of these elements has a fundamental role in various biological processes. The study revealed the importance of these elements in regulating the expression of OsGLPs that will help in predicting the role of these genes in various stresses and can have application in biotechnology.

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Expression Pattern and Promoter Analysis of a Eucalyptus grandis Germin-like Gene

Cited by 12 publications

References 34 publications

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Genome-Wide Characterization and Expression Analysis of the Germin-Like Protein Family in Rice and Arabidopsis

Functional characterization of the rice root Germin-like protein gene-1 (OsRGLP1) promoter in Nicotiana tabacum

In silico analysis of transcription factor binding sites in promoters of germin-like protein genes in rice

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