Expression pattern of AtABCC13/MRP11 reveals developmental, hormonal, and nutritional regulations

Guizani, Taissir El; Blanc, Nathalie; Triki, Saïda; St-Pierre, Benoit; Ducos, Eric

doi:10.1007/s10535-013-0387-0

Cited by 11 publications

(10 citation statements)

References 48 publications

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“…Earlier a PDR1-like gene was shown to be repressed by ABA treatment (Zhang et al, 2013 ) likewise in our case TaABCC6 and TaABCC13 were also repressed (Figure 5 ). Most of the wheat ABCC genes were highly induced by GA 3 , which is supported by previous expression pattern observed for AtABCC13 / ABCC11 for this hormone (Guizani et al, 2014 ). The differential expression response of wheat ABCC genes for hormone treatment will help in assimilating clues regarding their possible roles in integrated pathways that are involved in multiple abiotic stresses.…”

Section: Discussionsupporting

confidence: 85%

Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat

et al. 2015

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The ABCC multidrug resistance associated proteins (ABCC-MRP), a subclass of ABC transporters are involved in multiple physiological processes that include cellular homeostasis, metal detoxification, and transport of glutathione-conjugates. Although they are well-studied in humans, yeast, and Arabidopsis, limited efforts have been made to address their possible role in crop like wheat. In the present work, 18 wheat ABCC-MRP proteins were identified that showed the uniform distribution with sub-families from rice and Arabidopsis. Organ-specific quantitative expression analysis of wheat ABCC genes indicated significantly higher accumulation in roots (TaABCC2, TaABCC3, and TaABCC11 and TaABCC12), stem (TaABCC1), leaves (TaABCC16 and TaABCC17), flag leaf (TaABCC14 and TaABCC15), and seeds (TaABCC6, TaABCC8, TaABCC12, TaABCC13, and TaABCC17) implicating their role in the respective tissues. Differential transcript expression patterns were observed for TaABCC genes during grain maturation speculating their role during seed development. Hormone treatment experiments indicated that some of the ABCC genes could be transcriptionally regulated during seed development. In the presence of Cd or hydrogen peroxide, distinct molecular expression of wheat ABCC genes was observed in the wheat seedlings, suggesting their possible role during heavy metal generated oxidative stress. Functional characterization of the wheat transporter, TaABCC13 a homolog of maize LPA1 confirms its role in glutathione-mediated detoxification pathway and is able to utilize adenine biosynthetic intermediates as a substrate. This is the first comprehensive inventory of wheat ABCC-MRP gene subfamily.

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Section: Discussionsupporting

confidence: 85%

Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat

et al. 2015

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“…Furthermore, TaABCC3, a prime candidate for plant genetic engineering to enhance tolerance, was induced substantially by GA3 [66]. Similar results were also observed in other studies (AtABCC13/ABCC11) [69]. ABCC genes play a key role in integrated pathways under multiple abiotic stresses, and the effects of some other signaling molecules (i.e.…”

Section: Secondary Metabolites To Chelate and Sequestrate Cadmiumsupporting

confidence: 87%

Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue

Zhu

et al. 2020

BMC Genomics

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Background: It has been reported that nitric oxide (NO) could ameliorate cadmium (Cd) toxicity in tall fescue; however, the underlying mechanisms of NO mediated Cd detoxification are largely unknown. In this study, we investigated the possible molecular mechanisms of Cd detoxification process by comparative transcriptomic and metabolomic approaches. Results: The application of Sodium nitroprusside (SNP) as NO donor decreased the Cd content of tall fescue by 11% under Cd stress (T1 treatment), but the Cd content was increased by 24% when treated with Carboxy-PTIO (c-PTIO) together with Nitro-L-arginine methyl ester (L-NAME) (T2 treatment). RNA-seq analysis revealed that 904 (414 up-and 490 down-regulated) and 118 (74 up-and 44 down-regulated) DEGs were identified in the T1 vs Cd (only Cd treatment) and T2 vs Cd comparisons, respectively. Moreover, metabolite profile analysis showed that 99 (65 upand 34-down-regulated) and 131 (45 up-and 86 down-regulated) metabolites were altered in the T1 vs Cd and T2 vs Cd comparisons, respectively. The integrated analyses of transcriptomic and metabolic data showed that 81 DEGs and 15 differentially expressed metabolites were involved in 20 NO-induced pathways. The dominant pathways were antioxidant activities such as glutathione metabolism, arginine and proline metabolism, secondary metabolites such as flavone and flavonol biosynthesis and phenylpropanoid biosynthesis, ABC transporters, and nitrogen metabolism. Conclusions: In general, the results revealed that there are three major mechanisms involved in NO-mediated Cd detoxification in tall fescue, including (a) antioxidant capacity enhancement; (b) accumulation of secondary metabolites related to cadmium chelation and sequestration; and (c) regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the NO-mediated cadmium stress response.

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“…Furthermore, TaABCC3, a prime candidate for plant genetic engineering to enhance tolerance, was induced substantially by GA3 [73]. Similar results were also observed in other studies (AtABCC13/ABCC11) [76]. ABCC genes play a key role in integrated pathways under multiple abiotic stresses, and the effects of some other signaling molecules (i.e.…”

Section: Secondary Metabolites To Chelate and Sequestrate Cadmiumsupporting

confidence: 87%

Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue

Zhu

et al. 2020

Preprint

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Abstract Background: It has been reported that nitric oxide (NO) could ameliorate cadmium (Cd) toxicity in tall fescue; however, the underlying mechanisms of NO mediated Cd detoxification are largely unknown. In this study, we investigated the possible molecular mechanisms of Cd detoxification process by comparative transcriptomic and metabolomic approaches. Results: The application of Sodium nitroprusside (SNP) as NO donor decreased the Cd content of tall fescue by 11% under Cd stress (T1 treatment), but the Cd content was increased by 24% when treated with Carboxy-PTIO (c-PTIO) together with Nitro-L-arginine methyl ester (L-NAME) (T2 treatment). RNA-seq analysis revealed that 904 (414 up- and 490 down-regulated) and 118 (74 up- and 44 down-regulated) DEGs were identified in the T1 vs Cd (only Cd treatment) and T2 vs Cd comparisons, respectively. Moreover, metabolite profile analysis showed that 99 (65 up- and 34-down- regulated) and 131 (45 up- and 86 down-regulated) metabolites were altered in the T1 vs Cd and T2 vs Cd comparisons, respectively. The integrated analyses of transcriptomic and metabolic data showed that 81 DEGs and 15 differentially expressed metabolites were involved in 20 NO-induced pathways. The dominant pathways were antioxidant activities such as glutathione metabolism, arginine and proline metabolism, secondary metabolites such as flavone and flavonol biosynthesis and phenylpropanoid biosynthesis, ABC transporters, and nitrogen metabolism.Conclusions: In general, the results revealed that there are three major mechanisms involved in NO-mediated Cd detoxification in tall fescue, including (a) antioxidant capacity enhancement; (b) accumulation of secondary metabolites related to cadmium chelation and sequestration; and (c) regulation of cadmium ion transportation, such as ABC transporter activation. In conclusion, this study provides new insights into the NO-mediated cadmium stress response.

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Expression pattern of AtABCC13/MRP11 reveals developmental, hormonal, and nutritional regulations

Cited by 11 publications

References 48 publications

Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat

Genome-wide identification and expression characterization of ABCC-MRP transporters in hexaploid wheat

Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue

Comparative transcriptome combined with metabolome analyses revealed key factors involved in nitric oxide (NO)-regulated cadmium stress adaptation in tall fescue

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