Expression pattern of cellular nucleic acid-binding protein (CNBP) during embryogenesis and spermatogenesis of gibel carp

Abstract

By differential screening, we cloned the Cag-CNBP, demonstrated its predominant expression in ovary and testis, and reported its development behavior during folliculogenesis and oogenesis by immunofluorescence localization (Liu and Gui, Gene 365:181-192, 2005), but its developmental behavior during spermatogenesis and its transcript distribution during embryogenesis are not revealed. In the present study, by in situ hybridization, we analyze CagCNBP expression pattern during gibel carp embryogenesis. The CagCNBP transcripts ubiquitously distributed in all embryonic cells in early developmental stage embryos, and peak in midbrain, hindbrain and somites of gibel carp larva during organogenesis. By antibody detection, we reveal CagCNBP protein distribution change during spermatogenesis. The cell-specific distribution of CagCNBP is revealed by immunofluorescence staining, and predominant CagCNBP expression in testis somatic cells and spermatogonia is demonstrated in this paper. For the first time, the CNBP distribution during spermatogenesis in vertebrate has been revealed.

CNBP: A multifunctional nucleic acid chaperone involved in cell death and proliferation control

“…Several cell lines and adult animal tissues express CNBP mRNA at varying levels. However, the highest amounts of CNBP mRNA have been measured in testis (15) and ovary (16, 17), in which CNBP mRNA was detected in post‐vitellogenic oocytes (16, 18). These observations led to a detailed study of a role for CNBP during embryonic development.…”

“…The highest CNBP mRNA expression was observed in regions showing the highest cell proliferation rates. CNBP mRNA is expressed in the forebrain, midbrain, craniofacial structures, limb buds, and somites in the mouse embryo (3, 19) and in equivalent tissues of the chicken embryo (2), as well as at the border between midbrain and hindbrain, in prospective craniofacial structures, pectoral fins, retina, and liver of fish embryos (4, 15).…”

What's new about CNBP? Divergent functions and activities for a conserved nucleic acid binding protein

“…The significant amount of evidence suggesting that CNBP participates in transcriptional control [1,14,15,22,31,36,[38][39][40][41][42][43][44][45][46][47][48][49][50][51] reinforces the notion that CNBP is a nuclear protein. The nuclear localization was indeed detected by Western blot performed on nuclear extracts from mouse macrophages, splenocytes and lung epithelial carcinoma cells [22,36,37,45], as well as by immunolocalization in human [1,27,28,40,42,44,48] and mouse [22,45,49] cultured cells, fish gonads [19,52], and mice [38], fish [9,53], and amphibian embryonic cells [54].…”

Cellular nucleic acid-binding protein is vital to testis development and spermatogenesis in mice

“…In gibel carp testis, CNBP is expressed at high levels in spermatogonia and somatic cells, relatively low level in spermatocytes, and it is not expressed at all in sperm (Liu et al 2009b). However, the roles of CNBP during spermatogenesis remain elusive.…”