Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L.): Implications for the phytic acid biosynthetic pathway

Suzuki, Makoto; Tanaka, K.; Kuwano, Mio; Yoshida, Kaoru

doi:10.1016/j.gene.2007.09.006

Cited by 108 publications

(112 citation statements)

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“…2 and Table II). An earlier study suggested that the expression levels of the MIPS gene might be correlated with the accumulation of D-pinitol which is a cyclic sugar alcohol involved in the tolerance of environmental stress (Abreu and Aragão, 2007;Suzuki et al, 2007). Thus, our results indicated that the defensive system of plants regulates the expression levels and activity of rcMIPS in R. communis plants in order to enhance the defensive function against drought stress.…”

Section: Resultsmentioning

confidence: 50%

Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Wei

Dai²,

Wang³

et al. 2010

Zeitschrift Für Naturforschung C

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A novel 1 L-myo-inositol-1-phosphate synthase (MIPS, EC 5.5.1.4) gene, designate rcMIPS, was cloned from Ricinus communis. It contained an open reading frame (ORF) of 1669 bp coding for a peptide of 510 amino acids with a molecular mass of 56 kDa. Sequence anaylsis showed high homology compared to other plant MIPS genes, because it contained typical domains owned by MIPS enzymes. The transcript levels of the rcMIPS gene in leaves, stems, and roots were examined after drought stress for 24, 48, and 72 h. The transcript levels in the leaves, stems, and roots increased signifi cantly compared to the control. Results of the enzyme assay showed a signifi cant correlation between the changes of enzyme activity and the transcript levels of the rcMIPS gene in different organs. Decreased relative water contents (RWC) and increased malondialdehyde (MDA) contents in the leaves represented a stress response against drought stress. Our fi ndings suggest that MIPS plays an important role in the defensive mechanisms of R. communis against drought stress.

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Section: Resultsmentioning

confidence: 50%

Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Wei

Dai²,

Wang³

et al. 2010

Zeitschrift Für Naturforschung C

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“…A.MIPS(myo-inositol 3-phosphate synthase) B. IMP(inositol monophosphatase) C. IPK2(inositol1,4,5-tris phosphate kinase) D. IPK1(inositol 1,3,4,5,6-pentakis phosphate 2-kinase) E. ITP5/ 6 K(inositol 1,3,4-trisphophate 5/6-kinase). G6P:glucose 6-phosphate, Ins:myo-inositol, PtdIns:phophatidyl inositol (Suzuki et al 2007) The TILLING population was developed by Random mutations using Ethyl methanesulfonate (EMS) chemical mutagen agents for generation of low phytic acid content as well as high endogenous phytase activity showing mutants in Pusa Basmati rice (Shukla and Singh 2012). RNAi technology has been used to reduce maize phytic acid by silencing MRP4 ATP-binding cassette (ABC) transporter (Shi et al 2007;Gupta et al 2011).…”

Section: Screening Of Micronutrient Dense Germplasmmentioning

confidence: 99%

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

2013

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More than half of the world populations are affected by micronutrient malnutrition and one third of world's population suffers from anemia and zinc deficiency, particularly in developing countries. Iron and zinc deficiencies are the major health problems worldwide. Phytic acid is the major storage form of phosphorous in cereals, legumes, oil seeds and nuts. Phytic acid is known as a food inhibitor which chelates micronutrient and prevents it to be bioavailabe for monogastric animals, including humans, because they lack enzyme phytase in their digestive tract. Several methods have been developed to reduce the phytic acid content in food and improve the nutritional value of cereal which becomes poor due to such antinutrient. These include genetic improvement as well as several pre-treatment methods such as fermentation, soaking, germination and enzymatic treatment of grains with phytase enzyme. Biofortification of staple crops using modern biotechnological techniques can potentially help in alleviating malnutrition in developing countries.

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“…A complete elimination of the myo-inositol pathway through MIPS gene mutation stops the formation of phytic acid, but it also disrupts the many other biochemical pathways needed for germination. Indeed, several mutant MIPS lines of maize (Raboy and Cook, 2000), barley (Larson et al, 1998;Ockenden et al, 2004), rice (Larson et al, 2000;Suzuki et al, 2007) and soybean (Wilcox et al, 2000;Hitz et al, 2002) show a reduction of phytic acid levels.…”

Section: Myo-inositol Phosphate Synthase (Mips)mentioning

confidence: 99%

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

Abid

Silué

Muhovski

et al. 2009

Gene

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Received by A.J. van WijnenKeywords: myo-inositol phosphate synthase (MIPS) Sucrose synthase (Sus) Seed developmentThe aim of this review is to highlight the role of myo-inositol phosphate synthase (MIPS), which catalyses the first step in inositol biosynthesis and of sucrose synthase (Sus), an enzyme involved in UDP-glucose formation, the principal nucleoside diphosphate in the sucrose cleavage reaction and in trehalose biosynthesis. These two enzymes are involved in various physiological processes including seed growth and resistance to biotic and abiotic stresses. The study of mutated MIPS and Sus genes in some crops, such as soybean and cotton, has shown that these two proteins are directly involved in embryogenesis. They exhibit several isoforms that are essential for normal seed development. The possible role of both genes in seed development is discussed in this review.

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Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L.): Implications for the phytic acid biosynthetic pathway

Cited by 108 publications

References 50 publications

Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Cloning and Expression Analysis of 1 L-myo-Inositol-1-phosphate Synthase Gene from Ricinus communis L.

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

Role of myo-inositol phosphate synthase and sucrose synthase genes in plant seed development

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