Expression pattern of tumour necrosis factor receptors in subcutaneous and omental human adipose tissue: role of obesity and non‐insulin‐dependent diabetes mellitus

Hube, F; Birgel, M; Lee, Y M; Hauner, Hans

doi:10.1046/j.1365-2362.1999.00520.x

Cited by 106 publications

(84 citation statements)

References 24 publications

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“…versus s.c. adipose tissue. [34][35][36] In most cases, however, this difference seems to be attributable to increased cytokine or chemokine expression in the stromal-vascular fraction, so that our data are in line with other studies investigating isolated adipocytes and correcting for macrophage gene expression. 37,38 We also studied the effect of donor BMI on IP-10 release of mature adipocytes, but found only a nonsignificant trend towards increased IP-10 secretion in obese donors, whereas a statistically significant positive association between donor BMI and release of macrophage migration inhibitory factor has recently been demonstrated by us using the same methodology.…”

Section: Discussionsupporting

confidence: 90%

Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

et al. 2006

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Section: Discussionsupporting

confidence: 90%

Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

et al. 2006

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“…The adipose tissue expression of TNF-a mRNA has already been described in rodent models of obesity and also in humans (2,13). Subcutaneous fat depots exhibited 1.67-fold higher TNF-a mRNA expression Figure 4 Correlation between visceral adipocyte cell volume and (a) serum TNF-a, (b) serum sTNFR-2 and (c) fasting C-peptide concentrations in the subjects studied.…”

Section: Discussionmentioning

confidence: 77%

“…-have also been observed (5). Conflicting results concerning the differences in subcutaneous and visceral expression of TNF-a have been published (2,6,7).…”

Section: Introductionmentioning

confidence: 99%

Expression of tumor necrosis factor (TNF)-alpha protein in the subcutaneous and visceral adipose tissue in correlation with adipocyte cell volume, serum TNF-alpha, soluble serum TNF-receptor-2 concentrations and C-peptide level

Winkler

Kiss

Keszthelyi

et al. 2003

European Journal of Endocrinology

194

117

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Objective: The aim of the study was to evaluate the expression of tumor necrosis factor (TNF)-a protein in the subcutaneous and visceral adipose tissue in correlation with adipocyte cell volume, serum TNFa, soluble TNF-receptor-2 (sTNFR-2) and indirect parameters of insulin resistance in overweight/obese and lean healthy persons. Design: A cross-sectional case-control study was used. Patients: Twenty-eight overweight/obese probands with normal glucose tolerance (BMI . 27 kg/m 2 ) and 15 lean people (BMI , 25 kg/m 2 ), all of them undergoing planned surgical operation, participated in the study. Methods: Two to four grams of subcutaneous and visceral adipose tissue were removed and studied using semi-quantitative immunohistochemical staining of the TNF-a protein. Serum TNF-a, sTNFR-2 (ELISA) and fasting C-peptide (RIA) were measured. Results: TNF-a protein was expressed in adipocytes of both depots. The expression was evaluated visually and found to be greater in the obese patients. Significantly higher serum TNF-a (5.58^0.87 pg/ml vs 4.21^0.55, mean^S.D., P , 0.01, Mann -Whitney) and sTNFR-2 levels (7.84^3.56 ng/ml vs 4.59^1.35, P ¼ 0.005) were found in the obese subgroup in correlation with the fasting C-peptide level (r ¼ 0.49, P ¼ 0.003; and r ¼ 0.74, P ¼ 0.001) and the C-peptide/ blood glucose ratio (r ¼ 0.47, Spearman, P ¼ 0.005; and r ¼ 0.70, P ¼ 0.001). The cell volume of both adipocyte depots was found to have a significant positive correlation with serum TNF-a and sTNFR-2 levels in the total group of patients (subcutaneous: r ¼ 0.52, P ¼ 0.0003; r ¼ 0.69, P , 0.0001; visceral: r ¼ 0.65, P , 0.0001; r ¼ 0.63, P , 0.0001) and in both subgroups. Conclusions: Adipocyte cell volume of both the subcutaneous and visceral fat depots may be determinants of TNF-a, sTNFR-2 production and obesity-linked insulin resistance.

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“…Like leptin, TNF-a is known to be overexpressed in human obesity (28)(29)(30). Previous short-term in vivo studies showed a stimulatory effect of TNF-a on leptin gene expression and serum leptin concentrations in rodents after administration of TNF-a (14,15).…”

Section: Discussionmentioning

confidence: 99%

Depot-specific release of leptin from subcutaneous and omental adipocytes in suspension culture: effect of tumor necrosis factor-alpha and transforming growth factor-beta1

Gottschling-Zeller

Birgel

Scriba

et al. 1999

European Journal of Endocrinology

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Objective: Leptin, the product of the ob gene, is overexpressed in human obesity and increased serum leptin levels are closely correlated with adipose tissue mass, but the regulation of leptin production is not completely understood. The aim of this study was to characterize the role of tumor necrosis factor (TNF)-a and transforming growth factor (TGF)-b1 in depot-specific secretion of leptin from cultured human adipocytes. Design and methods: We measured the leptin concentrations in the culture medium of omental and subcutaneous abdominal adipocytes taken from severely obese individuals and kept in suspension culture, and studied the effect of TNF-a and TGF-b1 on leptin release. Leptin protein was measured by radioimmunoassay, leptin mRNA was assessed by reverse transcriptase (RT)-PCR relative to a housekeeping gene. Results and conclusion: Leptin secretion from subcutaneous fat cells was 2-to 3-fold higher than that from omental fat cells after incubation for 2 and 24 h respectively. A 2-h exposure of adipocytes to 1 nmol/l TNF-a and 400 pmol/l TGF-b1 respectively did not significantly affect leptin secretion. Whereas a 24-h incubation with 1 nmol/l TNF-a also did not influence leptin secretion from fat cells from both depots, exposure of omental fat cells to 400 pmol/l TGF-b1 for 24 h resulted in a significant inhibitory effect (by 33%) on leptin secretion (P<0.05). A 24-and 48-h exposure of in vitro differentiated human adipocytes to TNF-a led to a significant decrease in leptin mRNA levels to 70 Ϯ 8% and 49 Ϯ 13% of controls respectively. Similarly, TGF-b1 decreased leptin mRNA expression in newly differentiated human adipocytes to 77 Ϯ 12% after 24 h and to 54 Ϯ 8% after 48 h compared with control cultures. These data provide evidence that long-term exposure of human fat cells to TNF-a or TGF-b1 may suppress leptin expression in human adipose tissue. The inhibitory effect of TGF-b1 appears to be more pronounced in omental as compared with subcutaneous adipocytes.

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Expression pattern of tumour necrosis factor receptors in subcutaneous and omental human adipose tissue: role of obesity and non‐insulin‐dependent diabetes mellitus

Abstract: These results indicate that severe obesity in women is characterized by increased amounts of the two TNF receptor mRNAs. The role of this up-regulation for the development of obesity-associated insulin resistance remains to be elucidated.

Cited by 106 publications

References 24 publications

Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

Constitutive and regulated expression and secretion of interferon-γ-inducible protein 10 (IP-10/CXCL10) in human adipocytes

Expression of tumor necrosis factor (TNF)-alpha protein in the subcutaneous and visceral adipose tissue in correlation with adipocyte cell volume, serum TNF-alpha, soluble serum TNF-receptor-2 concentrations and C-peptide level

Depot-specific release of leptin from subcutaneous and omental adipocytes in suspension culture: effect of tumor necrosis factor-alpha and transforming growth factor-beta1

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