Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Zhang, Jian; Gao, Fang; Zhi, Huiying; Luo, Aiping; Ding, Fang; Wu, Min; Liu, Zhihua

doi:10.3748/wjg.v10.i8.1088

Cited by 8 publications

(6 citation statements)

References 28 publications

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“…Differential gene expression in development and in cancer transformation was compared by several research groups. [11][12][13]15 Very fruitful conclusions about correlations between genes differentially expressed during mouse fetal lung development and those differentially expressed in human lung cancer were obtained using an approach linking human cancerassociated genes with genes involved in rodent or other animal lung development. [11][12][13] A similar comparison of gene expression patterns was performed for human esophageal cancer and mouse embryonic esophagus.…”

Section: Introductionmentioning

confidence: 99%

“…[11][12][13] A similar comparison of gene expression patterns was performed for human esophageal cancer and mouse embryonic esophagus. 15 Despite obvious usefulness, comparisons of genes differentially expressed in human tumorigenesis and in the development of other mammalians have some serious limitations. Leaving aside technical problems, for example, that not all human genes have their orthologs on murine arrays, 11 there are two principal shortages: different timing of gene expression during fetal development in short-(such as rat and mouse) and long-gestation animals (such as humans or sheep), 13,16 and fundamental differences in tumorigenesis in mice and humans.…”

Section: Introductionmentioning

confidence: 99%

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Identification of some human genes oppositely regulated during esophageal squamous cell carcinoma formation and human embryonic esophagus development

Zinovyeva

Monastyrskaya

Kopantzev

et al. 2010

Diseases of the Esophagus

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Here we directly compared gene expression profiles in human esophageal squamous cell carcinomas and in human fetal esophagus development. We used the suppression subtractive hybridization technique to subtract cDNAs prepared from tumor and normal human esophageal samples. cDNA sequencing and reverse transcription polymerase chain reaction (RT-PCR) analysis of RNAs from human tumor and the normal esophagus revealed 10 differentially transcribed genes: CSTA, CRNN, CEACAM1, MAL, EMP1, ECRG2, and SPRR downregulated, and PLAUR, SFRP4, and secreted protein that is acidic and rich in cysteine upregulated in tumor tissue as compared with surrounding normal tissue. In turn, genes up- and downregulated in tumor tissue were down- and upregulated, respectively, during development from the fetal to adult esophagus. Thus, we demonstrated that, as reported for other tumors, gene transcriptional activation and/or suppression events in esophageal tumor progression were opposite to those observed during development from the fetal to adult esophagus. This tumor 'embryonization' supports the idea that stem or progenitor cells are implicated in esophageal cancer emergence.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Identification of some human genes oppositely regulated during esophageal squamous cell carcinoma formation and human embryonic esophagus development

Zinovyeva

Monastyrskaya

Kopantzev

et al. 2010

Diseases of the Esophagus

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“…Thus, overexpression of this protein was tumor specific and may be used for differential diagnosis from chronic pancreatitis. S100A8 is a differentiation-related gene that plays an important role in early embryonic development (35). Overexpression of S100A8 has been reported in various types of human cancers (36 -39).…”

Section: Proteomic Analysis Of Human Pancreatic Tissuesmentioning

confidence: 99%

Protein Expression Profiles in Pancreatic Adenocarcinoma Compared with Normal Pancreatic Tissue and Tissue Affected by Pancreatitis as Detected by Two-Dimensional Gel Electrophoresis and Mass Spectrometry

et al. 2004

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Pancreatic cancer is a rapidly fatal disease, and there is an urgent need for early detection markers and novel therapeutic targets. The current study has used a proteomic approach of two-dimensional (2D) gel electrophoresis and mass spectrometry (MS) to identify differentially expressed proteins in six cases of pancreatic adenocarcinoma, two normal adjacent tissues, seven cases of pancreatitis, and six normal pancreatic tissues. Protein extracts of individual sample and pooled samples of each type of tissues were separated on 2D gels using two different pH ranges. Differentially expressed protein spots were in-gel digested and identified by MS. Forty proteins were identified, of which five [i.e., ␣-amylase; copper zinc superoxide dismutase; protein disulfide isomerase, pancreatic; tropomyosin 2 (TM2); and galectin-1] had been associated previously with pancreatic disease in gene expression studies. The identified proteins include antioxidant enzymes, chaperones and/or chaperone-like proteins, calcium-binding proteins, proteases, signal transduction proteins, and extracellular matrix proteins. Among these proteins, annexin A4, cyclophilin A, cathepsin D, galectin-1, 14 -3-3, ␣-enolase, peroxiredoxin I, TM2, and S100A8 were specifically overexpressed in tumors compared with normal and pancreatitis tissues. Differential expression of some of the identified proteins was further confirmed by Western blot analyses and/or immunohistochemical analysis. These results show the value of a proteomic approach in identifying potential markers for early diagnosis and therapeutic manipulation. The newly identified proteins in pancreatic tumors may eventually serve as diagnostic markers or therapeutic targets.

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“…2003). Research into the molecular changes involved in development is important not only for understanding of embryogenesis, but also for understanding and managing disease (Zhang et al. 2004).…”

Section: Discussionmentioning

confidence: 99%

“…Cancer cells resemble embryonic cells morphologically and share some characteristics such as rapid proliferation, reduced differentiation and increased motility. The same intracellular signals that control proliferation and differentiation in development can control tumour development (Zhang et al. 2004), which makes these genes ideal for contributing towards understanding normal early embryo development.…”

Section: Discussionmentioning

confidence: 99%

Transcript Profiles of Some Developmentally Important Genes Detected in Bovine Oocytes and In Vitro‐produced Blastocysts Using RNA Amplification and cDNA Microarrays

Mamo

Sargent

Affara

et al. 2006

Reprod Domestic Animals

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To study the mRNA transcript profiles of some potential candidate developmental genes during bovine oocyte and blastocyst stages, RNA amplification procedures, cDNA microarray of 82 target genes spotted onto glass slide and real-time polymerase chain reaction (PCR) were used. Messenger RNAs were isolated from in vitro-produced bovine matured oocytes and blastocysts. Using equal amounts of input mRNAs but different cycles of amplifications, cDNAs were produced and served as template for RNA amplification by the in vitro transcriptions. After amplification, the RNA yields transcribed from cDNAs of different cycles were evaluated both by hybridization on the cDNA microarrays and by using real-time PCR techniques. The analyses indicated best results from lower amplification cycle templates with consistent signals at hybridization. Generally, the RNA yield was directly proportional to the amplification cycle but inversely related with signal consistency at repeated hybridizations. Using the protocols established, equal amounts of amplified RNA from matured oocytes and blastocysts were hybridized to the array. Analyses of replicated hybridizations indicated that 35 transcripts were differentially expressed. Most of these were not described in previous bovine embryo studies. Independent analyses of 23 transcripts with real-time PCR and unamplified RNA confirmed the results of 22 genes. Moreover, the functional analyses showed various roles related to development. Hence, it is possible to conclude that the genes identified here are potential candidates for characterizing developmental competence, and that the methods established can be used for large-scale gene expression analysis with more comprehensive arrays.

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Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Abstract: AIM:To investigate the expression patterns of esophageal squamous cell cancer deregulated genes in mid to late stages of C57BL/6J mouse embryogenesis, and the correlation between these genes in embryonic development and tumorigenesis of esophageal squamous cell cancer.

Cited by 8 publications

References 28 publications

Identification of some human genes oppositely regulated during esophageal squamous cell carcinoma formation and human embryonic esophagus development

Identification of some human genes oppositely regulated during esophageal squamous cell carcinoma formation and human embryonic esophagus development

Protein Expression Profiles in Pancreatic Adenocarcinoma Compared with Normal Pancreatic Tissue and Tissue Affected by Pancreatitis as Detected by Two-Dimensional Gel Electrophoresis and Mass Spectrometry

Transcript Profiles of Some Developmentally Important Genes Detected in Bovine Oocytes and In Vitro‐produced Blastocysts Using RNA Amplification and cDNA Microarrays

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