Expression patterns of PRDM10 during mouse embryonic development

Park, Jin‐Ah; Kim, Keun Cheol

doi:10.5483/bmbrep.2010.43.1.029

Cited by 25 publications

(17 citation statements)

References 19 publications

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“…The majority of PRDM proteins, including PRDM10, share a common structure including an N‐terminal PR domain, with potential methyltransferase activity, and multiple C2H2‐type zinc‐finger domains involved in sequence‐specific DNA‐binding. Based on a few animal models it may be involved in the embryonic development of both mesenchymal tissues and the central nervous system , and IHC studies have revealed widespread nuclear and cytoplasmic reactivity, with particularly strong positivity in renal tubules, the placenta, and gastrointestinal organs (https://www.proteinatlas.org/ENSG00000170325-PRDM10/).…”

Section: Discussionmentioning

confidence: 99%

Undifferentiated pleomorphic sarcomas with PRDM10 fusions have a distinct gene expression profile

Hofvander

Puls

Pillay

et al. 2019

The Journal of Pathology

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Undifferentiated pleomorphic sarcoma (UPS) is a highly aggressive soft tissue tumor. A subset of UPS is characterized by a CITED2-PRDM10 or a MED12-PRDM10 gene fusion. Preliminary data suggest that these so-called PRDM10-rearranged tumors (PRT) are clinically more indolent than classical high-grade UPS, and hence important to recognize. Here, we assessed the spectrum of accompanying mutations and the gene expression profile in PRT using genomic arrays and sequencing of the genome (WGS) and transcriptome (RNA-seq). The fusion protein's function was further investigated by conditional expression of the CITED2-PRDM10 fusion in a fibroblast cell line, followed by RNA-seq and an assay for transposase-accessible chromatin (ATAC-seq). The CADM3 gene was found to be differentially up-regulated in PRT and cell lines and was also evaluated for expression at the protein level using immunohistochemistry (IHC). The genomic analyses identified few and nonrecurrent mutations in addition to the structural variants giving rise to the gene fusions, strongly indicating that the PRDM10-fusions represent the critical driver mutations. RNA-seq of tumors showed a distinct gene expression profile, separating PRT from high-grade UPS and other soft tissue tumors. CADM3 was among the genes that was consistently and highly expressed in both PRT and fibroblasts expressing CITED2-PRDM10, suggesting that it is a direct target of the PRDM10 transcription factor. This conclusion is in line with sequencing data from ATAC-seq, showing enrichment of PRDM10 binding sites, suggesting that the amino-terminal fusion partner contributes by making the DNA more accessible to PRDM10 binding.

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Section: Discussionmentioning

confidence: 99%

Undifferentiated pleomorphic sarcomas with PRDM10 fusions have a distinct gene expression profile

Hofvander

Puls

Pillay

et al. 2019

The Journal of Pathology

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“…The gene encoding the transcription factor PRDM10 stands out for carrying HHMCs, being found in selective sweep regions and the second-most interacting protein within the HHMC dataset. Although little is known about PRDM10, it may be related to dendrite growth (Siegel et al 2002) and neural crest related changes that contributed to the formation of our distinct modern face (Park and Kim 2010). Other craniofacial morphology-related genes, such as DCHS2 (Adhikari et al (Rak 1986).…”

Section: The Craniofacial Phenotypementioning

confidence: 99%

A catalog of single nucleotide changes distinguishing modern humans from archaic hominins

Kuhlwilm

Boeckx

2018

Preprint

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10Throughout the past decade, studying ancient genomes provided unique insights into human 11 prehistory, and differences between modern humans and other branches like Neanderthals can 12 enrich our understanding of the molecular basis of the human condition. Modern human variation 13 and the interactions between different hominin lineages are now well studied, making it reasonable 14 to explore changes that are observed at high frequency in present-day humans, but do not reach 15 fixation. Here, we put forward interpretation of putative single nucleotide changes in recent modern 16 human history, focusing on 571 genes with non-synonymous changes at high frequency. We 17 suggest that molecular mechanisms in cell division and networks affecting cellular features of 18 neurons were prominently modified by these changes. Complex phenotypes in brain growth 19 trajectory and cognitive traits are likely influenced by these networks and other changes presented 20here. We propose that at least some of these changes contributed to uniquely human traits.

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“…It is a transcriptional regulator that has been implied in transduction of nerve growth factor signals via the p75 neurotrophin receptor and in cell growth arrest15, 17 and is expressed in a variety of tissues including the central and peripheral nervous systems 16, 18. Interestingly, we noticed that the PR domain in PRDM4 is preceded by a short motif that is also present in several other PR proteins including human PRDM6 (PRISM),13, 19 PRDM7,20 PRDM9 (meisetz),4, 21–23 PRDM10 (tristanin),24, 25 PRDM11, and PRDM1526 [Fig. 1(A,B)].…”

Section: Introductionmentioning

confidence: 95%