Expression profile analysis of 9 heat shock protein genes throughout the life cycle and under abiotic stress in rice

Abstract

Plant heat shock proteins (Hsps) facilitate protein folding or assembly under diverse developmental and adverse environmental conditions. Nine OsHsps were identified in our previous study from a proteomic analysis of rice cv. IRAT 109 leaf samples at the seedling stage under drought stress. To obtain additional information on the 9 OsHsp genes, this study focused on an expression profile analysis at different development stages throughout the life cycle of rice, and under different abiotic stresses and phytohormone treatments. The 9 genes exhibited distinctive expression patterns in different organs or development stages. Five of the genes (OsHsp72. 90, OsHsp72.57, OsHsp71.18, OsHsp24.15 and OsHsp18.03) showed high expression in the endosperm, indicating that OsHsp genes may play important roles in rice seed development. All 9 OsHsps were up-regulated under heat, polyethylene glycol, and abscisic acid treatment, whereas salt stress caused up-regulation of 6 genes (OsHsp93. 04, OsHsp71.10, OsHsp71.18, OsHsp72.57, OsHsp24.15 and OsHsp18.03) and cold stress resulted in down-regulation of OsHsp93.04 and OsHsp72.57. These diverse expression profiles imply potential functional diversity of the Hsp gene family in rice.heat shock protein, drought, heat, real-time PCR, rice

Molecular cloning and differential expression of sHSP gene family members from the resurrection plant Boea hygrometrica in response to abiotic stresses

“…It has been reported that endogenous ABA regulated HSP70 synthesis in the leaves and roots of maize seedlings exposed to drought, heat, and combined drought and heat stress, which implied that ABA may improve plant tolerance to stress by increasing HSP70 expression (Heikkila et al 1984;Cho et al 2006;Hu et al 2009;Ye et al 2012). Recently, ABA-inducible sHSP genes were also identified in rice and Capsella bursa-pastoris.…”

Overexpression of GmHsp90s, a Heat Shock Protein 90 (Hsp90) Gene Family Cloning from Soybean, Decrease Damage of Abiotic Stresses in Arabidopsis thaliana

“…In plants Hsp90 have been identified from Pharbitis nil [10], Brassica napus [11], [12], Secale cereale [13], Zea mays [14], Hordeum vulgare [15], Arabidopsis thaliana [16], [17], [18], Oryza sativa [19], [20], [21], Glycine max [22] and Vitis vinifera [23], and most are strongly induced by various abiotic stresses.…”

Expression of Target Gene Hsp70 and Membrane Stability Determine Heat Tolerance in Chili Pepper

“…HSP70 generally functions as a molecular chaperone; i.e., preventing the accumulation of and assisting in the re-folding of proteins under the conditions of stress. The role of HSP70 in relation to heat tolerance has been reported in several crop plants such as soybean [Glycine max (Ortiz and Cardemil, 2001)], wheat [Triticum aestivum (Duan et al, 2011)], tomato [Solanum lycopersicum (Fender and O'Connell, 1989)], and creeping bentgrass [Agrostis palustris (Ye et al, 2012)]. HSP70 also plays a regulatory role in the stress-associated expression of other genes (Lee and Sch€ offl, 1996).…”