Expression profile of arylphorin gene during diapause and cold acclimation in the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae)

Sonoda, Shoji; Fukumoto, Katsune; Izumi, Yohei; Ashfaq, Muhammad; Yoshida, Hideya; Tsumuki, Hisaaki

doi:10.1303/aez.2007.35

Cited by 7 publications

(5 citation statements)

References 18 publications

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“…The main functions of this protein are oxygen transport and nutrient reservoir activity. It might have an important role as a reservoir or cellular defence mechanism during diapause, as has been observed in other Lepidoptera [35]. …”

Section: Discussionmentioning

confidence: 97%

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

2012

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BackgroundSesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared under short day conditions enter diapause and undergo up to 12 molts before dying or pupating. To better understand the mechanism of larval development and diapause, we identified proteins with different expressions in the sixth instar of diapausing and non-diapausing larvae.ResultsA total of 52 differentially regulated proteins were detected in the hemolymph of the diapausing or non-diapausing larvae at the beginning or end of the sixth instar. From these proteins, 11 were identified by mass spectrometry (MALDI-TOF MS or MALDI-TOF/TOF MS/MS): 5 were upregulated in the hemolymph of non-diapausing larvae and 6 in the hemolymph of the diapausing larvae. Interestingly, some proteins were expressed only in non-diapausing larvae but none was expressed only in the hemolymph of diapausing larvae. The possible functions of some of these proteins related to diapause maintenance or to larval-pupal metamorphosis are discussed.ConclusionsThe 2-DE proteomic map of S. nonagrioides hemolymph shows differential protein expression in diapausing and non-diapausing larvae. Some proteins that showed higher expression in the diapausing larvae at the end of the sixth instar could be involved in JH level maintenance thus in the diapause status maintenance. On the contrary, other proteins that showed the highest expression or that were expressed only in the non-diapausing larvae could be involved in larval-pupal metamorphosis.

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Section: Discussionmentioning

confidence: 97%

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

2012

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“…Storage proteins are typically accumulated prior to diapause, and during diapause their constituent amino acids may be used for both anabolic activities in maintaining turnover of active protein pools, such as molecular chaperones, and catabolic respiratory metabolism, as well as supporting anabolic and catabolic roles in postdiapause functions such as resumption of development (36). Several studies indicate that storage proteins may continue to be synthesized at low levels during diapause (42,100). Whether storage proteins produced during diapause are playing roles in amino acid storage and intermediary metabolism or some other function, such as humoral defense or cold tolerance, is not known, but their rapid disappearance following diapause termination suggests a role in postdiapause tissue remodeling.…”

Section: Nutrient Storage In Preparation For Diapausementioning

confidence: 99%

Energetics of Insect Diapause

Hahn

Denlinger

2011

Annu. Rev. Entomol.

650

632

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Managing metabolic resources is critical for insects during diapause when food sources are limited or unavailable. Insects accumulate reserves prior to diapause, and metabolic depression during diapause promotes reserve conservation. Sufficient reserves must be sequestered to both survive the diapause period and enable postdiapause development that may involve metabolically expensive functions such as metamorphosis or long-distance flight. Nutrient utilization during diapause is a dynamic process, and insects appear capable of sensing their energy reserves and using this information to regulate whether to enter diapause and how long to remain in diapause. Overwintering insects on a tight energy budget are likely to be especially vulnerable to increased temperatures associated with climate change. Molecular mechanisms involved in diapause nutrient regulation remain poorly known, but insulin signaling is likely a major player. We also discuss other possible candidates for diapause-associated nutrient regulation including adipokinetic hormone, neuropeptide F, the cGMP-kinase For, and AMPK.

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“…Field-collected larvae were exposed to long photoperiods and high temperatures in the laboratory to terminate diapause, which contrasts with previous studies involving extreme endocrine or pharmacological manipulation. Previous studies mainly focused on comparisons of non-diapausing and diapausing states [ 20 , 24 , 25 , 26 ], or clarifying the threshold of environmental stimuli to induce or terminate diapause [ 16 , 19 , 27 ]. The results of our study provide a roadmap to understand the physiological and molecular mechanisms associated with insect diapause termination, especially in Lepidoptera.…”

Section: Discussionmentioning

confidence: 99%

Time-Series Transcriptomic Analysis Reveals the Molecular Profiles of Diapause Termination Induced by Long Photoperiods and High Temperature in Chilo suppressalis

Bao

Zhu

et al. 2022

IJMS

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Survival and adaptation to seasonal changes are challenging for insects. Many temperate insects such as the rice stem borer (Chilo suppressalis) overcome the adverse situation by entering diapause, wherein development changes dynamically occur and metabolic activity is suppressed. The photoperiod and temperature act as major environmental stimuli of diapause. However, the physiological and molecular mechanisms that interpret the ecologically relevant environmental cues in ontogenetic development during diapause termination are poorly understood. Here, we used genome-wide high-throughput RNA-sequencing to examine the patterns of gene expression during diapause termination in C. suppressalis. Major shifts in biological processes and pathways including metabolism, environmental information transmission, and endocrine signalling were observed across diapause termination based on over-representation analysis, short time-series expression miner, and gene set enrichment analysis. Many new pathways were identified in diapause termination including circadian rhythm, MAPK signalling, Wnt signalling, and Ras signalling, together with previously reported pathways including ecdysteroid, juvenile hormone, and insulin/insulin-like signalling. Our results show that convergent biological processes and molecular pathways of diapause termination were shared across different insect species and provided a comprehensive roadmap to better understand diapause termination in C. suppressalis.

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Expression profile of arylphorin gene during diapause and cold acclimation in the rice stem borer, Chilo suppressalis Walker (Lepidoptera: Crambidae)

Cited by 7 publications

References 18 publications

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides

Energetics of Insect Diapause

Time-Series Transcriptomic Analysis Reveals the Molecular Profiles of Diapause Termination Induced by Long Photoperiods and High Temperature in Chilo suppressalis

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