Expression profile of caseins, estrogen and prolactin receptors in mammary glands of dairy ewes

Colitti, Monica; Pulina, Giuseppe

doi:10.4081/ijas.2010.e55

Cited by 12 publications

(17 citation statements)

References 37 publications

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“…In contrast to our findings in lactating cows, the relative expression level of LALBA in ewes reached the highest value only at the end of lactation. Furthermore, Colitti and Pulina (2010) analyzed transcripts of the four caseins CSN1S1, CSN1S2, CSN2, and CSN3 in mammary tissue after slaughter in dairy ewes. Respective to the study of Colitti and Pulina (2010), gene expression of the four caseins was up-regulated during peak, mid-, and late lactation but down-regulated during pregnancy and involution.…”

Section: Milk Protein Gene Expressionmentioning

confidence: 99%

“…Furthermore, Colitti and Pulina (2010) analyzed transcripts of the four caseins CSN1S1, CSN1S2, CSN2, and CSN3 in mammary tissue after slaughter in dairy ewes. Respective to the study of Colitti and Pulina (2010), gene expression of the four caseins was up-regulated during peak, mid-, and late lactation but down-regulated during pregnancy and involution. Those findings correspond to ovine milk protein composition during lactation.…”

Section: Milk Protein Gene Expressionmentioning

confidence: 99%

“…In numerous previous studies on mRNA expression, mammary gland tissue was obtained at one or at few time points via intricate biopsies (Farr et al, 1996;Finucane et al, 2008) or once after slaughtering of precious experimental animals (Capuco et al, 2001;Colitti and Pulina, 2010). In those samples, mRNA was extracted directly from all cells or after a preceding step of pBMEC cultivation (Talhouk et al, 1990;Griesbeck-Zilch et al, 2008).…”

Section: Cell Isolationmentioning

confidence: 99%

“…They found the highest expression levels for both on day 60 postpartum (pp) Loor, 2007, 2011, respectively). In lactating dairy ewe mammary gland expression levels of the caseins (CSN1S1, CSN1S2, CSN2, CSN3) did not change, but during involution and late pregnancy the expression levels lowered (Colitti and Pulina, 2010). Bevilacqua et al (2006) investigated the expression levels and the translation efficiency of the four caseins in goat, ewe, and cow.…”

mentioning

confidence: 99%

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Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

Sigl¹,

Meyer²,

Wiedemann³

2012

CZECH J ANIM SCI

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ABSTRACT:The objective of the present study was to refine a previously developed method to isolate primary bovine mammary epithelial cells (pBMEC) from fresh milk. Using this method, it was tested whether the number of pBMEC and the relation of recovered pBMEC to total somatic cell count vary within the individual lactation stages. Furthermore, the expression levels of the milk protein genes during the first twenty weeks of lactation were determined by quantitative PCR method. A total number of 152 morning milk samples were obtained from twenty-four Holstein-Friesian cows during the first 20 weeks of lactation (day 8, 15, 26, 43, 57, 113, and 141 postpartum). Numbers of extracted pBMEC were consistent at all time-points (1.1 ± 0.06 to 1.4 ± 0.03 ×10 3 /ml) and an average value of RNA integrity number (RIN) was 6.3 ± 0.3. Percentage of pBMEC in relation to total milk cells (2.0 ± 0.2 to 6.7 ± 1.0%) correlated with milk yield. Expression patterns of the casein genes alpha (α) S1 , (α) S2 , beta (β), and kappa (κ) (CSN1S1, CSN1S2, CSN2, CSN3, respectively) and the whey protein genes α-lactalbumin (LALBA) and progestagen-associated endometrial protein (PAEP; known as β-lactoglobulin) were shown to be comparable, i.e. transcripts of all six milk protein genes were found to peak during the first two weeks of lactation and to decline continuously towards mid lactation. However, mRNA levels were different among genes with CSN3 showing the highest and LALBA the lowest abundance. We hypothesized that milk protein gene expression has a pivotal effect on milk protein composition with no influence on milk protein concentration. This paper is the first to describe milk protein gene expression during lactation in pBMEC collected in milk. Future studies will be needed to understand molecular mechanisms in pBMEC including regulation of expression and translation throughout lactation.

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Section: Milk Protein Gene Expressionmentioning

confidence: 99%

Section: Milk Protein Gene Expressionmentioning

confidence: 99%

Section: Cell Isolationmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

Sigl¹,

Meyer²,

Wiedemann³

2012

CZECH J ANIM SCI

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“…Furthermore, Bionaz and Loor () found that the CSN3 gene expression pattern was more or less similar to the lactation curve in the MT of cows. On the other hand, Colitti and Pulina () observed no differences on the expression of caseins' genes in dairy ewes among 30, 60 and 150 DIM, when took MT samples from different animals throughout the lactation period. Additionally, Colitti and Farinacci (), by taking MT from different ewes too, found that the expression of LALBA gene reached the highest value at the end of lactation (150 DIM).…”

Section: Discussionmentioning

confidence: 96%

The effect of long‐term under‐ and overfeeding on the expression of six major milk proteins' genes in the mammary tissue of goats

Tsiplakou

Flemetakis

Kouri

et al. 2015

Animal Physiology Nutrition

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Milk protein synthesis in the mammary gland involves expression of six major milk proteins' genes whose nutritional regulation remains poorly defined. In this study, the effect of long-term under- and overfeeding on the expression of as1-casein: CSN1S1, as2-casein: CSN1S2, β-casein: CSN2, κ-casein: CSN3, α-lactalbumin: LALBA and β-lactoglobulin: BLG gene in goat mammary tissue (MT) was examined. Twenty-four lactating dairy goat, at 90-98 days in milk, were divided into three homogenous subgroups and fed the same ration, for 60 days, in quantities which met 70% (underfeeding), 100% (control) and 130% (overfeeding) of their energy and crude protein requirements. The results showed a significant decrease in mRNA of CSN1S2, CSN2, CSN3 and LALBA genes in the MT of underfed goats compared with the overfed and on the CSN1S1 and BLG gene expressions in the MT of underfed goats compared with the respective control and overfed. CSN2 was the most abundant transcript in goat MT relative to the other milk proteins' genes. Significantly positive correlations were observed between the mRNA levels of caseins' and BLG genes with the milk yield. Moreover, a significant correlation was found between the mRNA levels of CSN1S2 with the milk protein, lactose content and lactose yield and also between the LALBA gene expression with the lactose content and lactose yield respectively. In conclusion, the feeding level and consequently the nutrients availability affected the milk lactose content, protein and lactose yield as well as the milk volume by altering the CSN1S1, CSN1S2, CSN2, CSN3, LALBA and BLG gene expression involved in their metabolic pathways.

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Immunolocalization of estrogen and progesterone receptors in ewe mammary glands

Colitti

Parillo

2013

Microscopy Res & Technique

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Immunopresence of estrogen receptor a (ERa), b (ERb) and progesterone receptor (PR) were examined in the ewe mammary gland from prepubertal stage to involution. Immunolocalization of ERa revealed a strong positive staining in nuclei of cells composing terminal ductular units (TDUs) in prepubertal ewes. A mild immunoreactivity was identified in early lactating gland. During late lactation immunoreactive product to ERa was observed in the cytoplasm of glandular epithelial cells in all alveoli. In mammary glands at involution ERa positivity was clearly nuclear, with weak to moderate cytoplasmic staining. Cytoplasmic strong immunostaining for ERb was detected in cells of TDUs, whereas some stromal cells exhibited nuclear staining. A nuclear ERb immunostaining was observed at early lactation, instead during late lactation, the positivity for ERb showed only a moderate cytoplasmic distribution. At involution, ERb positivity was very moderate and detected just in the cytoplasm of shrunken alveoli. Scattered nuclear staining of PR was observed just in mammary glands at early lactation. These results showed that in the mammary glands of sheep both estrogen receptor isoforms were displayed during lactation cycle and that PR appeared just at early lactation, reflecting their regulatory role in alveolar cells. Microsc.

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Expression profile of caseins, estrogen and prolactin receptors in mammary glands of dairy ewes

Cited by 12 publications

References 37 publications

Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

Gene expression of six major milk proteins in primary bovine mammary epithelial cells isolated from milk during the first twenty weeks of lactation

The effect of long‐term under‐ and overfeeding on the expression of six major milk proteins' genes in the mammary tissue of goats

Immunolocalization of estrogen and progesterone receptors in ewe mammary glands

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