Expression Profile of Laccase Gene Family in White-Rot Basidiomycete Lentinula edodes under Different Environmental Stresses

Abstract

Laccases belong to ligninolytic enzymes and play important roles in various biological processes of filamentous fungi, including fruiting-body formation and lignin degradation. The process of fruiting-body development in Lentinula edodes is complex and is greatly affected by environmental conditions. In this paper, 14 multicopper oxidase-encoding (laccase) genes were analyzed in the draft genome sequence of L. edodes strain W1-26, followed by a search of multiple stress-related Cis-elements in the promoter region of these laccase genes, and then a transcription profile analysis of 14 laccase genes (Lelcc) under the conditions of different carbon sources, temperatures, and photoperiods. All laccase genes were significantly regulated by varying carbon source materials. The expression of only two laccase genes (Lelcc5 and Lelcc6) was induced by sodium-lignosulphonate and the expression of most laccase genes was specifically upregulated in glucose medium. Under different temperature conditions, the expression levels of most laccase genes decreased at 39 °C and transcription was significantly increased for Lelcc1, Lelcc4, Lelcc5, Lelcc9, Lelcc12, Lelcc13, and Lelcc14 after induction for 24 h at 10 °C, indicating their involvement in primordium differentiation. Tyrosinase, which is involved in melanin synthesis, was clustered with the same group as Lelcc4 and Lelcc7 in all the different photoperiod treatments. Meanwhile, five laccase genes (Lelcc8, Lelcc9, Lelcc12, Lelcc13, and Lelcc14) showed similar expression profiles to that of two blue light receptor genes (LephrA and LephrB) in the 12 h light/12 h dark treatment, suggesting the involvement of laccase genes in the adaptation process of L. edodes to the changing environment and fruiting-body formation. This study contributes to our understanding of the function of the different Lelcc genes and facilitates the screening of key genes from the laccase gene family for further functional research.

Lacase de Agaricus subrufescens cultivado em meio com melaço de cana-de-açúcar promove a descoloração de corantes sintéticos

“…que não fora expressa na ausência Research, Society andDevelopment, v. 9, n. 12, e12391210942, 2020 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v9i12.10942 deste indutor. Em fungos, a produção de isoenzimas é comum e genes de lacase se organizam em famílias com vários genes (Yan et al, 2019). O corante triarilmetano VM foi descolorido mais rapidamente por A. subrufescens U7-1 do que por A. subrufescens .…”

Genome-wide characterization of laccase gene family in Schizophyllum commune 20R-7-F01, isolated from deep sediment 2 km below the seafloor

“…However, some fungal strains have no signal peptide sequence in the laccase gene. For instance, LeLAC3 of Lentinula edodes contained a signal peptide sequence in strain D703PP-9, but was absent in strain W1-26 ( Sakamoto et al, 2015 ; Yan et al, 2019 ). The deficiency of signal peptide sequence was also reported in Flammulina velutipes and Setosphaeria turcica ( Wang et al, 2015 ; Liu et al, 2019 ), as well as in plants ( Xu et al, 2019 ).…”

Gllac7 Is Induced by Agricultural and Forestry Residues and Exhibits Allelic Expression Bias in Ganoderma lucidum

“…Considering the vital function of laccase in lignin degradation, we conducted a comprehensive analysis toward laccase gene family of G. lucidum . Macro-fungi species usually contain more than 10 laccase genes, e.g., 14 in Lentinula edodes ( Yan et al, 2019 ), 12 in Pleurotus ostreatus ( Jiao et al, 2018 ), and 11 in Flammulina velutipes ( Wang et al, 2015b ). And it is assumed that the presence of large gene families of laccases in fungal genomes could be explained by broad spectrum of enzyme’s physiological functions required during the life cycle of a fungus ( Kües and Rühl, 2011 ).…”