Expression profile of several genes in human myometrium and uterine leiomyoma

Roth, Ted M.; Klett, Christoph; Cowan, Bryan D.

doi:10.1016/j.fertnstert.2006.10.001

Cited by 14 publications

(10 citation statements)

References 30 publications

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“…Of relevance to this investigation, it is interesting to note that genes associated with steroid hormone regulation, the TGFB pathway and genes involved in the RA pathway make up a significant portion of genes that are differentially expressed in fibroid tissue compared with myometrium (refer to Table 1 for a brief summary; Sadan et al 1987, Dou et al 1996, Tsibris et al 2002, Skubitz & Skubitz 2003, Weston et al 2003, Shozu et al 2004, Arslan et al 2005, Leppert et al 2006, Zaitseva et al 2006, Roth et al 2007, Litovkin et al 2008, Lee et al 2010, Moore et al 2010, Davis et al 2013, Yin et al 2013.…”

Section: Introductionmentioning

confidence: 99%

Common fibroid-associated genes are differentially expressed in phenotypically dissimilar cell populations isolated from within human fibroids and myometrium

Holdsworth-Carson¹,

Zaitseva²,

Girling³

et al. 2014

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Uterine fibroids are a prevalent gynaecological condition in reproductive-aged women and are the commonest reason for hysterectomy. The cellular composition of clonal fibroids are heterogeneous, with phenotypically dissimilar cells that include smooth muscle cells (SMC), vascular SMC (VSMC) and fibroblasts. The aim of our study was to investigate genes that are commonly differentially expressed between fibroid and myometrial whole tissues in phenotypically different sub-populations of cells isolated from fibroid and myometrium. Genes to be investigated by fluorescence-activated cell sorting, quantitative real-time PCR and immunocytochemistry include transforming growth factor b (TGFB) and retinoic acid (RA) signalling families and steroid hormone receptors. We hypothesised that each cell population isolated from fibroid and myometrium would differ in the expression of fibroid-associated genes. We demonstrated that phenotypically different cellular constituents of uterine fibroids differentially express cellular RA-binding protein 2 (CRABP2), progesterone receptor B (PRB) and TGFB receptor 2 mRNA in fibroid-derived cells of VSMC and SMC phenotype. CRABP2 mRNA was also differentially expressed in fibroblasts and VSMC sub-populations from within clonal fibroid tumours. We conclude that differential regulation of RA, TGFB and PR pathway transcription occurs in fibroid-associated SMC and -fibroblasts and that investigation of paracrine interactions between different cell types within the fibroid microenvironment provides an important new paradigm for understanding the pathophysiology of this common disease.

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Section: Introductionmentioning

confidence: 99%

Common fibroid-associated genes are differentially expressed in phenotypically dissimilar cell populations isolated from within human fibroids and myometrium

Holdsworth-Carson¹,

Zaitseva²,

Girling³

et al. 2014

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“…In recent years, a variety of technologies, including differential hybridization, differential display polymerase chain reaction (DD-PCR) (4), representational difference analysis (5), suppression subtractive hybridization (SSH) (6), and gene microarray (7)(8)(9)(10), have been used to identify the genes selectively altered in uterine leiomyoma. All of these methods are used to analyze the uterine leiomyoma-relative genes at mRNA expression levels.…”

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confidence: 99%

Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics

Zhu

Dong

et al. 2008

Fertility and Sterility

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“…What distinguishes the leiomyomata from its surrounding myometrium is the excessive and disorganized ECM that surrounds the cells. The excessive ECM secreted by the leiomyoma cells in vivo is supported by the altered expression of the genes specific to these ECM proteins, as compared with the myometrium (1,(22)(23)(24)(25)(26). We have demonstrated that the immortalized myometrium and leiomyoma cells, when grown in 3D collagen-1 culture, retain the in vivo characteristics of the tissues they are derived from as well as the characteristic spindle shape of the SMC.…”

Section: Discussionmentioning

confidence: 84%

Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium

Malik

Catherino

2012

Fertility and Sterility

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Expression profile of several genes in human myometrium and uterine leiomyoma

Cited by 14 publications

References 30 publications

Common fibroid-associated genes are differentially expressed in phenotypically dissimilar cell populations isolated from within human fibroids and myometrium

Common fibroid-associated genes are differentially expressed in phenotypically dissimilar cell populations isolated from within human fibroids and myometrium

Reduced expression of 14-3-3 gamma in uterine leiomyoma as identified by proteomics

Development and validation of a three-dimensional in vitro model for uterine leiomyoma and patient-matched myometrium

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