Expression profiles of Pr5<scp>CS</scp>1 and Pr5<scp>CS</scp>2 genes and proline accumulation under salinity stress in perennial ryegrass (Lolium perenne L.)

Li, Huiying; Guo, Huijuan; Zhang, Xunzhong; Fu, Jinmin

doi:10.1111/pbr.12140

Cited by 9 publications

(4 citation statements)

References 41 publications

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“…Previous studies have also demonstrated the correlation between the induction of P5CS gene and the accumulation of proline in sorghum under abiotic stresses and MeJA treatment (Su et al 2011). For ryegrass, Li et al (2014) found a high cause-effect relationship between the increase in transcripts of both isoforms of P5CS and the proline content, which was upregulated after stress. In rice plants, Hur et al (2004) found that the expression of gene P5CS1 was constant, while P5CS2 was induced by mannitol and NaCl.…”

Section: Discussionmentioning

confidence: 82%

Correlation of proline content and gene expression involved in the metabolism of this amino acid under abiotic stress

et al. 2016

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This study evaluated the metabolism of proline in rice based on the expression pattern of genes involved in biosynthesis and catabolism of amino acid and determined the correlation with the proline content produced under stress. In stage V4, genotypes with a differentiated response to salinity and low-temperature stresses were exposed separately to two test conditions: 150 mM NaCl and 13°C. Proline increased in both genotypes and stresses. The expression of P5CS1 was increased in both genotypes and conditions, while P5CS2 was responsive in the sensitive genotype under salinity. High correlations between proline content/P5CS1, proline content/P5CS2, P5CS1/P5CR, and P5CS2/P5CR were observed for the tolerant genotype under salt stress. The correlation between proline content/PDH was 0.42 and-0.13 (tolerant genotype) and-0.54 and 0.15 (sensitive genotype) under salinity and cold, respectively. P5CDH was positively correlated with proline content under salinity for both genotypes. Under salt stress, there was a positive correlation between proline content/OAT in the tolerant genotype. It was concluded that the increase in proline content is greater for the tolerant genotype. Under salt stress, the levels of transcript genes are more correlated with the proline content in the tolerant genotype, while at 13°C, the correlation of the sensitive genotype was higher.

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Section: Discussionmentioning

confidence: 82%

Correlation of proline content and gene expression involved in the metabolism of this amino acid under abiotic stress

et al. 2016

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“…Expression analysis revealed that in perennial ryegrass, the upregulation of P5CS genes can be induced by salinity stress and may be associated with salt-stress tolerance (Li et al, 2014). In this work, the relative expression of P5CS in perennial ryegrass was the highest in the salt treatment alone, which was downregulated when treated with WTH and JLH to different extents in the presence of salt stress.…”

Section: Discussionmentioning

confidence: 48%

Humic acids enhance salt stress tolerance associated with pyrroline 5-carboxylate synthetase gene expression and hormonal alteration in perennial ryegrass (Lolium perenne L.)

Meng,

Yan,

Zhang

et al. 2023

Front. Plant Sci.

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Humic acid (HA) has been used as an important component in biostimulant formulations to enhance plant tolerance to salt stress, but the mechanisms underlying are not fully understood. This study was to investigate the physiological and molecular mechanisms of HA’s impact on salt stress tolerance in perennial ryegrass (Lolium perenne L.). The two types of HA were extracted from weathered coal samples collected from Wutai County (WTH) and Jingle County (JLH) of Shanxi Province, China. The grass seedlings subjected to salt stress (250 mM NaCl) were treated with HA solutions containing 0.01% WTH (W/V) or 0.05% JLH (W/V), respectively. The HA treatments improved leaf photosynthetic rate (Pn), transpiration rate (Tr), and stomatal conductance (Gs) and reduced leaf oxidative injury (lower malondialdehyde content) and Pro and intercellular CO2 concentrations in salt-stressed perennial ryegrass. The HA treatments also reversed the decline in antioxidative enzymes ascorbate peroxidase (APX), catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) activity and improved growth and anti-senescence hormones indole-3-acetic acid (IAA) and brassinosteroid (BR). The HA treatments reduced the relative expression of P5CS and its downstream products proline (Pro) and the stress defense hormones abscisic acid (ABA), salicylic acid (SA), jasmonic acid (JA), and polyamines (PA). The results of this study indicate that the application of HAs may improve salt stress tolerance by regulating P5CS gene expression related to osmotic adjustment and increasing the activity of antioxidant enzymes and anti-senescence hormones in perennial ryegrass.

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“…Furthermore, Li et al isolated two salt-induced P5CS genes ( P5CS1 and P5CS2 ) from perennial ryegrass by using rapid-amplification of cDNA ends PCR (RACE-PCR). The accumulation of proline, which is significantly induced after salt treatment, is in line with the expression levels of those two P5CS genes under high salinity treatment [ 20 ], suggesting that both P5CS genes may be involved in the salt stress tolerance of perennial ryegrass.…”

Section: Salt Stressmentioning

confidence: 89%

“…These abiotic stresses are sometimes seasonal, but increasingly unpredictable due to climate change [ 19 ]. Therefore, abiotic stresses such as drought, high salinity, and extreme temperatures are major restrictive factors in perennial ryegrass growth and management [ 12 , 17 , 20 ]. Breeding stress-resistance cultivars is considered as an important measure to mitigate the effects of abiotic stress on perennial ryegrass [ 17 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms

Miao

Zhang

Bai

et al. 2022

Life

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Perennial ryegrass (Lolium perenne L.) is an important turfgrass and gramineous forage widely grown in temperate regions around the world. However, its perennial nature leads to the inevitable exposure of perennial ryegrass to various environmental stresses on a seasonal basis and from year to year. Like other plants, perennial ryegrass has evolved sophisticated mechanisms to make appropriate adjustments in growth and development in order to adapt to the stress environment at both the physiological and molecular levels. A thorough understanding of the mechanisms of perennial ryegrass response to abiotic stresses is crucial for obtaining superior stress-tolerant varieties through molecular breeding. Over the past decades, studies of perennial ryegrass at the molecular and genetic levels have revealed a lot of useful information to understand the mechanisms of perennial ryegrass adaptation to an adverse environment. Unfortunately, molecular mechanisms by which perennial ryegrass adapts to abiotic stresses have not been reviewed thus far. In this review, we summarize the recent works on the genetic and molecular mechanisms of perennial ryegrass response to the major abiotic stresses (i.e., drought, salinity, and extreme temperatures) and discuss new directions for future studies. Such knowledge will provide valuable information for molecular breeding in perennial ryegrass to improve stress resistance and promote the sustainability of agriculture and the environment.

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Expression profiles of Pr5CS1 and Pr5CS2 genes and proline accumulation under salinity stress in perennial ryegrass (Lolium perenne L.)

Cited by 9 publications

References 41 publications

Correlation of proline content and gene expression involved in the metabolism of this amino acid under abiotic stress

Correlation of proline content and gene expression involved in the metabolism of this amino acid under abiotic stress

Humic acids enhance salt stress tolerance associated with pyrroline 5-carboxylate synthetase gene expression and hormonal alteration in perennial ryegrass (Lolium perenne L.)

Insights into the Response of Perennial Ryegrass to Abiotic Stress: Underlying Survival Strategies and Adaptation Mechanisms

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