Expression Profiles of Somatotropic Axis Genes in Lines of Chickens Divergently Selected for 56-Day Body Weight

Wu, Guiqin; Siegel, P. B.; Gilbert, Elizabeth R.; Yang, Ning; Wong, Eric A.

doi:10.1080/10495398.2011.566450

Cited by 15 publications

(6 citation statements)

References 42 publications

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“…With advance of molecular genetics, it becomes possible to significantly improve fish production through detection of polymorphisms in genes affecting and regulating growth such as, growth hormone gene (GH), insulin like growth factors (IGF-I, IGF-II) genes, myostatin (MSTN-1), prolactin (PRL), and somatolactin (SL). This called genetic selection which is an effective tool for molecular marker assisted selection (MAS) (Lei et al 2007;Wu et al 2011). The MAS increases the selection precision and reduce the number of generations required to achieve a desired improvement.…”

Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Khatab¹,

Hemeda²,

El‐Nahas³

et al. 2014

AJVS

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Insulin-like growth factor II (IGF-II) is a crucial factor in regulating cell proliferation, growth, migration, differentiation and survival. The objective of this study was to investigate the association of polymorphism in intron 3 and exon 3 of insulin like growth factor II (IGF-II) gene and body weight trait in tilapia nilotica fish using restriction fragment length polymorphism (RFLP) and single nucleotide polymorphism (SNP) techniques. Thirty tilapia nilotica fish were precisely selected according to their body weight and ordered from the largest to the smallest body size. DNA was extracted from fish blood samples to amplify 397-bp of IGF-II gene. Amplified IGF-II gene was sequenced only in the seven large and five small size fish. Results revealed that restriction analysis (RFLP) with MSP1 of the (IGF-II) gene (397-bp) do not produce restriction fragments. While, DNA sequencing showed fourteen single nucleotide polymorphisms (SNP's) at different positions in large size fish as they were detected in more than one fish at nucleotides number 318 (C→T), 319 (A→T), 320 (G→A in three fish), 322 (C→A and C→G), 323 (G→C and G→A), 326 (G→A in three fish), 332 (A→T in three fish), 333 (C→A and C→T), 334 (A→C in two fish), 337 (C→T in two fish) and 340 (C→T). Hence, our results reveal the possibility to use these findings as marker for selection of high body weight trait in tilapia nilotica fish.

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Section: Introductionmentioning

confidence: 99%

Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Khatab¹,

Hemeda²,

El‐Nahas³

et al. 2014

AJVS

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“…The insulin-like growth factor (IGF) system, consisting of 2 growth factors (IGF-I and IGF-II), 2 IGF receptors [IGF-IR and IGF-IIR/cation-independent mannose 6-phosphate receptor (M6PR)], and 7 IGF-binding proteins (IGFBP1-7), has been characterized as an important regulatory system for controlling tissue growth and development in vertebrates (Richards et al, 2005;Castigliego et al, 2010;Wu et al, 2011). IGF can regulate cellular proliferation and differentiation through the endocrine and auto/paracrine systems (Jones and Clemmons, 1995;Simmen et al, 1998;Liu and LeRoith, 1999;Xu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks

Song

Liu²,

Kou³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (post-hatching weeks 1-8) in ducks. The results showed a significant age-related change in mRNA in these muscle tissues. In breast muscle, the developmental expression of IGF-I and IGF-II was highest during week 1 but decreased quickly and maintained a relatively lower level. Leg muscle had the highest mRNA expression of IGF-I and IGF-II genes at week 3. In myocardial tissues, the expression level of IGF-IR and IGF-IIR genes exhibited a "rise-decline" developmental trend. The expression patterns of IGF-I/ IGF-IR and IGF-II/IGF-IIR were different between weeks 4 and 6. The same expression pattern was observed for IGF-I and IGF-IR; however, it was different from that observed for IGF-II and IGF-IIR. Our results showed a negative correlation between IGF-II mRNA expression and leg muscle weight at week 4 (P < 0.05). A negative correlation was also found between IGF-II mRNA expression and breast muscle weight (P < 0.01), and a positive correlation was found between IGF-IR expression and breast muscle weight. At week 6, a positive correlation was found between IGF-IR expression and breast muscle weight. However, at week 8, a negative correlation was found between IGF-IR expression and breast muscle weight. The results showed that the expression of IGF mRNA in duck tissues exhibits a specific developmental trend and an age-related pattern, suggesting that the regulation mechanism of these 4 genes in proliferation and differentiation of muscle tissues differed.

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“…Despite an apparent increase in the number of pituitary somatotrophs in fast-growing lines of birds during late embryonic development [29], it is well established that circulating GH is elevated in slower-growing lines of chickens after hatch [30, 31], and differences observed in pituitary GH mRNA levels in the current study were consistent with this. Pituitary GH mRNA was also shown to be higher in lighter birds 4 weeks after hatch using a similar genetic model system in which HWS birds weigh approximately 10-fold more than LWS birds at 8 weeks of age [8]. It has been demonstrated in different birds from these same lines that circulating GH levels were 2.5-fold higher in LG chickens than HG chickens (unpublished observation by LAC, TEP, JS, and MJD).…”

Section: Discussionmentioning

confidence: 99%

“…These findings are similar to those reported earlier in sex-link dwarf ( dw/dw ) chickens, which have elevated plasma GH, no detectable hepatic GH-binding activity, and maintain two-thirds of the plasma IGF-1 levels despite lacking a functional GHR gene [32–35]. In contrast, hepatic GHR mRNA expression was observed to be higher early post-hatch in LWS chickens and only modestly reduced at 4 weeks of age when compared to HWS chickens, and it was reported that GHR mRNA levels in breast muscle are higher in LWS birds during both embryogenesis and post-hatch [8]. Others have found that breast muscle cells from LG birds exhibit reduced sensitivity to IGF1 stimulation [18], and it has been suggested that their visceral fat may have a reduction of IGF1 signaling when compared to HG birds [15].…”

Section: Discussionmentioning

confidence: 99%

“…They have been widely used to investigate growth, metabolism, and feed intake in broiler chickens. Alterations in expression of components of the somatotropic axis, including minor differences in pituitary GH expression [8], and muscle regulatory genes [9] have been observed between HWS and LWS birds. Metabolic differences between these genetic groups are highlighted by alterations in pancreatic function, glucose homeostasis, and metabolic flux in skeletal muscle and adipose tissue [10–12].…”

Section: Introductionmentioning

confidence: 99%

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Transcriptional profiling and pathway analysis reveal differences in pituitary gland function, morphology, and vascularization in chickens genetically selected for high or low body weight

et al. 2019

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Background Though intensive genetic selection has led to extraordinary advances in growth rate and feed efficiency in production of meat-type chickens, endocrine processes controlling these traits are still poorly understood. The anterior pituitary gland is a central component of the neuroendocrine system and plays a key role in regulating important physiological processes that directly impact broiler production efficiency, though how differences in pituitary gland function contribute to various growth and body composition phenotypes is not fully understood. Results Global anterior pituitary gene expression was evaluated on post-hatch weeks 1, 3, 5, and 7 in male broiler chickens selected for high (HG) or low (LG) growth. Differentially expressed genes (DEGs) were analyzed with gene ontology categorization, self-organizing maps, gene interaction network determination, and upstream regulator identification to uncover novel pituitary genes and pathways contributing to differences in growth and body composition. A total of 263 genes were differentially expressed between HG and LG anterior pituitary glands ( P ≤ 0.05 for genetic line-by-age interaction or main effect of line; ≥1.6-fold difference between lines), including genes encoding four anterior pituitary hormones. Genes involved in signal transduction, transcriptional regulation, and vesicle-mediated transport were differentially expressed and are predicted to influence expression and secretion of pituitary hormones. DEGs involved in immune regulation provide evidence that inflammation and response to cellular stressors may compromise pituitary function in LG birds, affecting their ability to adequately produce pituitary hormones. Many DEGs were also predicted to function in processes that regulate organ morphology and angiogenesis, suggesting pituitary gland structure differs between the divergently selected lines. Conclusions The large number of DEGs within the anterior pituitary gland of birds selected for high or low body weight highlights the importance of this gland in regulating economically important traits such as growth and body composition in broiler chickens. Intracellular signaling, transcriptional regulation, and membrane trafficking are important cellular processes contributing to proper hormone production and secretion. The data also suggest that pituitary function is intimately tied to structure, and organization of the gland could influence hypothalamic and systemic metabolic inputs and delivery of hormones regulating growth and metabolism into peripheral circulation. Electronic supplementary material The online version of this article (10.1186/s12864-019-5670-9) contains supplementary material, which is available to authorized users.

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Expression Profiles of Somatotropic Axis Genes in Lines of Chickens Divergently Selected for 56-Day Body Weight

Cited by 15 publications

References 42 publications

Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Genetic Polymorphism in IGF-II Gene and Its Relationship with Growth Rate in Tilapia Nilotica

Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks

Transcriptional profiling and pathway analysis reveal differences in pituitary gland function, morphology, and vascularization in chickens genetically selected for high or low body weight

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