Expression Profiling of microRNA From Peripheral Blood of Dairy Cows in Response to Staphylococcus aureus-Infected Mastitis

Abstract

As the main pathogen causing dairy cow mastitis, Staphylococcus aureus can cause subclinical mastitis, which is difficult to be diagnosed. It seriously affects milk quality and the economic benefits of the dairy industry. Therefore, it is very necessary to find biomarkers for early diagnosis of S. aureus-infected mastitis in peripheral blood of dairy cows. In this study, S. aureus was used to infect the mammary gland tissues of dairy cows, and a mastitis model was successfully constructed. The RNAseq technology was used to determine the expression profiles of microRNA (miRNA) from peripheral blood of dairy cows infected with S. aureus at 0, 1, 3, 5, and 7 days. A total of 288 differentially expressed miRNAs (DIE-miRNAs) were found, of which 108 were known miRNAs and 180 were novel predicted miRNAs. Bioinformatics analysis results showed that the above DIE-miRNAs might be involved in 10 immune system-related signaling pathways (i.e., chemokine signaling pathway, leukocyte transendothelial migration, natural killer cell-mediated cytotoxicity, toll-like receptor signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Wnt signaling pathway, cell adhesion molecules, cytokine-cytokine receptor interaction, and ECM-receptor interaction), thus regulating the process of S. aureus mastitis. It was also found that the expression variation of up-regulated expression of miR-320a, miR-19a, and miR-19b as well as down-regulated expression of miR-143, miR-205, and miR-24 reached a significant level on the 5th and 7th day of infection, suggesting that they might play an important biological role in mastitis and provide a direction for the research and development of molecular therapy technology for mastitis. However, at different times after S. aureus infection, miR-1301 was significantly up-regulated in peripheral blood. miR-2284r was significantly down-regulated, suggesting that these two miRNAs might be the new blood biomarkers for S. aureus-infected dairy cow mastitis. The above results laid a new foundation for the research and development of molecular diagnosis and biological therapy technology for S. aureus-infected mastitis in dairy cow.

Integrated Analysis of Transcriptome mRNA and miRNA Profiles Reveals Self-Protective Mechanism of Bovine MECs Induced by LPS

“…Additionally, 29 top-expressed miRNAs account for 81, 84, 83, and 85% in the expression of total miRNAs at 0, 4, 8, and 12 h cells, respectively (Figure 4), which are similar to the previous results in bovine MAC-T cells challenged with E. coli or S. aureus (16). The expression of bta-miR-21-5p_R-1 is more than 30% in total miRNAs and is the highest one (Figure 4), which is also in line with the result in primary bMECs (15), while bta-miR-21-5p_R-1 is not the highest expressed miRNAs at peripheral blood in Chinese Holstein cows (23,24), bovine milk-derived exosomes (22), bovine milk (21), and bovine mammary glands (19). This indicated that bta-miR-21-5p_R-1 was the unique and essential miRNA to maintain the function of bMECs.…”

“…Characterization of the transcriptomic response to infection in different tissues is an effective approach to understanding the response mechanism of dairy mastitis. In the past decades, a large number of mastitis-related mRNAs and miRNAs have been identified by the Transcriptome-Seq in infected bMECs (9,(15)(16)(17), udder tissues (18)(19)(20), milk whey (21), milkderived exosomes (22), whole blood (23)(24)(25) and blood-isolated monocytes (26) in the context of S. aureus, Streptococcus uberis, Streptococcus agalactiae, and E. coli. MECs have a strong capacity to mount differing innate immune responses to gram-positive and gram-negative bacterial challenges in their own way.…”

Investigating circulating miRNA in transition dairy cows: What miRNAomics tells about metabolic adaptation

“…The Wnt pathway consists of a large family of highly conserved glycoproteins that cross-talk with MAPK in development, determining cell fate, tissue regeneration, and maintaining tissue homeostasis ( Zhang et al, 2014 ). Previous studies have shown that Wnt and MAPK pathways are essential for normal mammary gland development ( Roarty and Serra, 2007 ; Li et al, 2018 ) and are involved in immune system-related signaling pathways ( Luoreng et al, 2021 ). These studies reported candidate miRNA including bta-miR-19a, bta-miR-19b, bta-miR-21-5p, bta-miR-29c, bta-miR-143, bta-miR-145, and bta-miR-146b are associated with Wnt signals and MAPK pathway ( Li et al, 2018 ; Luoreng et al, 2021 ).…”

Differential Expression Profiles of lncRNA Following LPS-Induced Inflammation in Bovine Mammary Epithelial Cells

“…Cow mastitis is an inflammatory disease of mammary tissue. Often, it is caused by the invasion and proliferation of pathogenic microorganisms, physical damage, chemical stimulation and the cow's immune response (1,2,37). However, among these causes, E. coli is the most common culprit (38,39).…”

“…Mastitis is one of the most common diseases in dairy cows worldwide, causing damage to mammary tissue, reduces milk production, decreases the quality of dairy products, and increases mortality and culling rates (1,2). Although mastitis is a common disease, it is both expensive and difficult to treat, resulting in huge losses in the dairy industry (3)(4)(5).…”