Expression, Purification, and Characterization of the Human Receptor Activator of NF-κB Ligand (RANKL) Extracellular Domain

Willard, Derril H.; Chen, Wenji; Barrett, George B.; Blackburn, Kevin; Bynum, Jane; Consler, Thomas G.; Hoffman, Christine R.; Horne, Earnest; Iannone, Marie A.; Kadwell, Sue H.; Parham, Janet H.; Ellis, Byron

doi:10.1006/prep.2000.1278

Cited by 20 publications

(18 citation statements)

References 34 publications

(27 reference statements)

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“…An early study suggested that another TNF family cytokine, TRAIL, may also interact with OPG (37), although the physiological relevance of this observation is still unclear. RANKL binds to OPG with an affinity of 6.7 nM as measured by surface plasmon resonance (38). In contrast, the affinity of TRAIL for OPG is reported to be only K D = 400 nM (39), even though TRAIL binds to its membrane receptor DR5 with a K D = 2 nM under similar conditions.…”

Section: Discussionmentioning

confidence: 99%

Crystal structure of the TRANCE/RANKL cytokine reveals determinants of receptor-ligand specificity

Lam

Nelson²,

Ross³

et al. 2001

J. Clin. Invest.

169

117

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Section: Discussionmentioning

confidence: 99%

Crystal structure of the TRANCE/RANKL cytokine reveals determinants of receptor-ligand specificity

Lam

Nelson²,

Ross³

et al. 2001

J. Clin. Invest.

169

117

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“…The pFB-RANK-L truncate was a generous gift from GlaxoSmithKline, and its preparation and characterization were published previously (18). The wild type pFB-OPG-H (His 6 ) was a generous gift from GlaxoSmithKline, and its preparation was similar to that published for the pFB-OPG-Fc (18). The pFB-C ϩ RANK-L construct was engineered with an N-terminal cysteine for Biacore surface coupling using PCR to insert the additional codon.…”

Section: Production Of Recombinant Viruses and Proteinmentioning

confidence: 99%

“…Elution occurred at 200 -300 mM NaCl. T. ni-derived human OPG-IgG fusion (OPG-Fc), RANK-L, and OPG-H pure proteins were purified as described (18) and were provided as a generous gift from D. Willard, GlaxoSmithKline, Research Triangle Park, NC.…”

Section: Production Of Recombinant Viruses and Proteinmentioning

confidence: 99%

“…Osteoclastogenesis as measured in such a way was inhibited in a competitive manner by OPG (5,23). Direct binding of RANK-L to a osteoprotegerin-IgG Fc domain fusion (OPG-Fc) has also been measured and reported to exhibit a K D of 6.7 nM, using surface plasmon resonance (18).…”

mentioning

confidence: 99%

“…All of our studies used the globular ectodomain of RANK-L, a trimer at concentrations above 40 pM (18). This form has been shown to contain the receptor-binding module and to fold independently of the remainder of the protein (7,9).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Functional Dissection of Osteoprotegerin and Its Interaction with Receptor Activator of NF-κB Ligand

Schneeweis

Willard

Milla

2005

Journal of Biological Chemistry

Self Cite

109

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RANK-Fc: A therapeutic antagonist for RANK-L in myeloma

Sordillo

Pearse

2003

Cancer

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Steady flows of gas and particles in unbounded‐fluidized beds have been shown to lose stability to localized voidage disturbances. An insight is provided into the linear stability of bounded gas‐particle flows using a kinetic theory approach. The stability analysis is carried out for two illustrative sets corresponding to fluidized systems having walls acting as sinks and sources of fluctuation energy. Instabilities are characterized by computing leading eigenvalue profiles and dominant eigenfunction contour maps. Results show two types of dominant instability patterns (symmetric and antisymmetric) for flow in a vertical duct, both of which propagate through the bed in the form of traveling waves at speeds comparable to that of the solid phase. Moreover, model predictions show that increasing solids fraction and decreasing particle inelasticity will suppress the wavy disturbance. The physical mechanism of instability formation is further investigated using a term‐by‐term method of analysis. Results show that instability modes can be suppressed if solid phase inertia or inelastic particle collisions are eliminated from the momentum and pseudo‐thermal energy balance equations. Finally, the symmetric instabilities were shown to develop into bubble‐like structures while the antisymmetric instabilities develop into streamer‐like structures. © 2007 American Institute of Chemical Engineers AIChE J, 2007

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Expression, Purification, and Characterization of the Human Receptor Activator of NF-κB Ligand (RANKL) Extracellular Domain

Cited by 20 publications

References 34 publications

Crystal structure of the TRANCE/RANKL cytokine reveals determinants of receptor-ligand specificity

Crystal structure of the TRANCE/RANKL cytokine reveals determinants of receptor-ligand specificity

Functional Dissection of Osteoprotegerin and Its Interaction with Receptor Activator of NF-κB Ligand

RANK-Fc: A therapeutic antagonist for RANK-L in myeloma

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